Microscope Axio Imager.Z2m complete system

Axio Imager.Z2m microscope stand with Z-drive mot. and TFT monitor - heavy duty focusing drive motorized, stepsize 10 nm, with fine drive knob and fine drive disk, flat with scala, changeable - reflected-light illumination mot. with shutter and diffusion disk - Light Control mot. - Main controller incl. USB-A, USB-C and TCP/IP interface - external 12V DC 100W power unit, stabilized, 100V...240V AC/ 50...60Hz/230VA - USB cable and country-specific line cable

7-position objective nosepiece, HD M27 mot.

Transmitted-light/reflected-light stage carrier, detachable for Axio Imager suitable for sample heights from 0 mm up to 45 mm

Double filter wheel, discrete, mot., 2x4 positions each to accept neutral-density filter set and dia. 25 mm color filters. For use in transmitted-light and reflected-light equipments mot.

6-position reflector turret mot. ACR, for P&C modules with automatic filter set and contrast module recognition

Stop slider man. with centerable luminous-field diaphragm slider stop position can be set

Stop slider with centerable aperture stop

Reflector module polarizer ACR P&C for reflected light

Reflector module bright field ACR P&C for reflected light

Illuminator microLED 3 for mounting on the lamp interface for transmitted-light or reflected-light illumination - colour temperature 5700 K - colour rendering index >90

Scanning Stage 130x85 STEP (D) for Axio Imager - stepper motor with 2 mm spindle pitch - connection to stepper controller - travel range 130 mm x 85 mm - max speed 50 mm/s - resolution: 0.1 µm - reproducibility: +/- 1 µm - absolute accuracy: +/- 5 µm - travel range adjustable - fixed motor cable with exit on stage back side; left - for mounting frames 160 x 116 mm and Z-PIEZO

Holder TCA for filter membrane, DM 47 (D) Holder for filter membrane, diameter 47 mm. Accessory for analysis of particulate contamination on filter membranes.

Mounting frame for one holder TCA (D) The mounting frame is accessory for technical cleanliness and applicable for: - holder TCA for filter membranes with diameter 47 mm; - holder TCA for filter membranes with diameter 50 mm; - holder TCA for the test standard.

Binocular phototube 15°/25 (100:0/0:100), upright image adjustable stop, camera port with interface 60N

Conversion filter 5700-3200 K, d=25 mm for adapting the LED light to the color impression of halogen light

Neutral-density filter set D/A, d=25 attenuation up to 0.0028 % possible. For integration in filter wheels, discrete, man. or mot. Not usable for HBO/XBO

Camera Adapter 60N-C 2/3" 0.63x; rotating +/- 2° for fine adjustment of camera rotation. - adjustment range: +/- 2° - maximum weight load: 2 kg

Stage micrometer for reflected light 5 + 100/100y d=0mm - 5 lines with 1mm intervals in y-direction - 100 lines with 1/100mm intervals in y-direction

Eyepiece PL 10x/23 Br. foc.

Eyepiece eyecup

Objective EC Epiplan 10x/0.25 M27 (FWD=11.0mm)

Objective EC Epiplan 5x/0.13 M27 (FWD=11.8mm)

Stage Controller XY STEP SMC 2009 Control unit for Maerzhaeuser XY-Stages with stepper motors. - bidirectional communication to microscopes with CAN - position display on microscopes with TFT display and option for objective depended travel speed - 2 CAN interfaces - alternative connection through USB or RS232 - power supply 100...240V AC/50...60Hz - incl. USB-cable, CAN-cable and plug-in power unit with country specific power cord

Joystick XY; CAN (D) for mechanical stages mot; CAN, scanning stages; CAN and stage controller XY STEP SMC 2009 - 2 speed ranges selectable - direct cable connection to CAN stage or to stage controller XY STEP SMC 2009

Microscopy Workstation Premium (HP Z8 G5) (O) HP Z8 Fury G5 Workstation - Chipset: Intel W790 - Memory Expansion Slots: 16 x DDR5, RDIMM, ECC, 4800 MHz Modules - Memory: max. 1024 GB RAM - PCI Express Connectors: 2 x PCI Express Generation5 x16 1 x PCI Express Generation 5 x4 2 x PCI Express Generation 4 x16 2 x PCI Express Generation 4 x4 1 x PCI Express Generation 3 x8 - SSD: 1 x 2 TB M.2 NVMe - Hard Drives: 2 x 12 TB SATA 7200 rpm (configured as 10 TB RAID 1hard drive) - Network Controller: 2 x GbE LAN interface (RJ 45) - USB: 2 x USB 3.2 Gen2x2 type C (front), 8 x USB 3.1 Gen 1 type A (2 x front, 6 x rear) - Certifications <(>&<)> Declarations: CE, CSA, UL, CCC, KCC - Power Supply: 100 - 240 V / 50 - 60 Hz / 1450 W with country specific plug - Chassis Dimensions: (H x W x D): 445 x 216 x 555 mm - USB Mouse, Recovery kit

Processor Intel Xeon W5-3425 (HP Z8 G5) (O) Default processor configuration - Intel Xeon W5-3425 (3.2 GHz, 30 MB cache, 12 cores) - only for 410203-9914-000 Microscopy Workstation Premium HP Z8 Fury G5

Memory 32 GB (1x32) DDR5 4800 MHz ECC reg RAM (HP Z8 G5) (O) consisting of 1 x 32 GB module - DDR5 4800 MHz ECC registered DIMM - can be extended to 64 GB (2 x 410303-3206-000), 128 GB (4 x 410303- 3206-000) or 256 GB (8 x 410303-3206-000) - only for 410203-9914-000 Microscopy Workstation Premium HP Z8 Fury G5

Graphics Card NVIDIA RTX A2000 12 GB (O) Professional graphics card for Microscopy Workstation - 12 GB GDDR6 memory - PCI Express 4.0 Interface x16 - Hardware acceleration of DirectX 12.07, OpenGL 4.68 and CUDA - Support for up to four displays - Display outputs: 4 x MiniDisplayPort 1.4 (mDP) - DisplayPort support for 4 x 32" Display (3840 x 2160 Pixel)

System Configuration 2 for HP Z8 G5 (O) Installation of Windows 10 IoT Enterprise LTSC 2021 x64 incl. Windows 10 License - with ZEN software installation and system integration of respective system - for Celldiscoverer 7, LSM 900, LSM 980, Elyra 7, Lattice SIM, Axioscan 7, Lattice Lightsheet - only for 410203-9914-000 Microscopy Workstation Premium HP Z8 Fury G5

Language Pack English US (O) - Keyboard with English (US) layout

Monitor TFT 24" HP Z24n G3 (61 cm) (O) Visible diagonal: 61 cm, 16:10 Maximum resolution: 1920 x 1200 pixel @ 60 Hz Brightness: 350 nits Contrast: 1000:1 Viewing angle: horizontal 178 degree, vertical 178 degree Video input: DisplayPort 1.2, HDMI 1.4 Data connectors: 1 x USB Type B (upstream), 4 x SuperSpeed USB Type-A 5Gbps Power consumption: Maximum 63 W, Typical 28 W, Standby 0.5 W Dimensions incl. stand (H x W x D): 52.1 cm x 53.2 cm x 19.5 cm Weight incl. stand: 4.9 kg Ergonomic features:Tilt: -5°to +20°, Swivel: -45° to +45°, Height adjustable 15 cm, Pivot rotation: -90° to +90° Certification and compliance: CE, CB, GS, TÜV, FCC, TCO Operating voltage: 100-240 V, country-specific power cable

Microscopy Camera Axiocam 705 pol R2 (D) High Performance microscopy camera incl. driver software 64 bit USB 3.0 PCIe x1 interface, USB 3.0 cable 3 m and protection glass (wideband coated) Sensor Type: SONY IMX250 MZ Exmor CMOS, SONY Pregius Global Shutter Monochrome with 2x2 pixel specific polarization filter mask (90°, 45°, 135°, 0°) Number of Pixels: 2464 (H) x 2056 (V) = 5.07 Mega pixel Pixel Size: 3.45 µm x 3.45 µm Chip Size: 8.5 mm x 7.1 mm, equivalent to 2/3" (diagonal 11.1 mm) Spectral Range: With protection glass approx. 350 nm to 1000 nm Full Well Capacity: Approx. 11,000 e at gain 1x (typ.) Adjustable Signal Amplification: 1x, 2x, 4x, 8x, 16x Selectable Resolution: H x V Binning Factor (1x1) 2464 x 2056 1x1 Basic Resolution 816 x 684 3x3 480 x 410 5x5 (at 2x2 combined analog and digital binning for further increase of sensitivity) Live Frame Rates: (1 ms exposure, default setting, simplified color coding) H x V Frame Rate (fps) 2464 x 2056 25 (1ms exposure time, ROI 1920 x 1080, standard color coding) H x V Frame Rate (fps) 1920 x 1080 5,5 Read Noise Full Well Capacity Dynamic Range (at gain) 2.20 e (1x) 11,000 e 1:5000 1.74 e (2x) 5,000 e 1:3100 1.48 e (4x) 2,700 e 1:1800 1.29 e (8x) 1,300 e 1:1300 1.15 e (16x) 690 e 1: 600 Software Supported Camera Operation: Exposure time adjustment with continuous, automatic or manual mode, adjustable over or under-exposure mode, optional measurement on defined target area (spot- meter) Readout of Sensor Sub Regions ("ROI"): Adjustable Digitization: 8, 12, 14 bit / pixel (8 out of 14 bit or compression mode) Dark Current: <(><<)>0.5 e/p/s @ 18 °C sensor temperature Cooling: Stabilized temperature to 18 °C (for ambient temperatures from 18 °C to 30 °C) Exposure Time Range: 0.1ms to 60 s Hardware Trigger: Trigger-In for exposure time control, Trigger-Out for exposure time, readout time and trigger ready status (further accessories required) Status-LED for Camera: Color coded operation status, dimmable Camera Interface: USB 3.0 (Optional operation withUSB 2.0 at reduced frame rate) Optical Interface: C-Mount Thread Depth for Objectives: max. 5 mm Size / Weight: Approx. 10.8 cm x 7.8 cm x 5,1 cm (2.3"x 3.2" x 2,0") / 580 g Housing: blue anodized aluminum, with cooling fins on top, 1/4" photo thread for tripod mount Registration: CE Power Supply: Provided by USB 3.0 und USB 2.0, max. 7 W consumption Environmental Conditions: 5 °C to 35 °C, max. 80% relative air humidity, not condensing, free air circulation required Supported Operating Systems: for ZEN: Windows 10 x64 Supported Application Software: ZEN 3.6 (blue edition, Hotfix 3.6.6) or higher, ZEN core 3.5 (Hotfix 3.5.4) or higher The required application software is available via the ZEISS Download Center.

ZEN core 3.9 Software suite for microscope image acquisition, processing, analysis, and data connectivity. - Configurable graphical user interface including workbench concept for customized routine jobs and workflows - Full integration of the ZEISS microscopes (incl. Visioner 1) that can be configured in MicroToolbox, Axiocam cameras and additional components - Interactive and automatic control of the individual motorized microscope components and transfer of information from encoded components into the software - Image acquisition with b/w, rgb, high-resolution and high-sensitivity cameras - HDR, Panorama, and manual Extended Depth of Focus (EDF) acquisitions - Basic image acquisition on electron microscopes - Image processing: standard operations for image optimization (contrast, brightness, gamma, colors, smoothing, sharpening, geometric corrections...) - Interactive/dimensional measurements, including online measurements on the live image - Input Form Designer with automated customer data import - Report creation and modification of Microsoft Word report templates using ZEN Word add-in - Data Archive for managing and maintaining documents: searching, filtering, tagging, viewing, re-opening, structuring, exporting, importing (any file format via drag n drop) - User Management for multi-user environment incl. full integration into the Windows multi-user functionality - Connectivity to ZEN Data Storage image database - Contains ZEN Connect basic functionality, 2D CAD overlay, topography viewer for 2.5D visualization of EDF Images incl. profile line measurement, QUAL Data Export - arivis Cloud connector enables the execution of customized solutions by integrating modules from the arivis Cloud platform Supported operating systems: Windows 10 (64-Bit)

ZEN Module Technical Cleanliness Analysis Software module for evaluation of particulate contamination on prepared specimen. The following standards for component cleanliness, oil cleanliness, air cleanliness and cleanliness of medical products are supported: VDA 19.1, ISO 16232, ISO 4406, ISO 4407, NAS 1638, SAE AS 4059,VDA 19.2, VDI 2083 (Sheet 21). The scope of delivery includes preconfigured automated workflows for technical cleanliness analysis and rating of single or multiple specimen for the user roles Supervisor and Operator. Provided job templates (I-V): Workflow Creator Wizard for application guided selection and auto-generation of the following job templates for technical cleanliness analysis: - The workflows I - III are operable with acquired or loaded images. - The correlative workflows IV - V require image acquisition. (I) automated workflow for acquisition of multichannel images with different polarization directions in combination with Axiocam 705 pol mainly for the evaluation of component cleanliness and the rating of technical cleanliness of medical products. The use of latest camera sensor technology with on-chip polarization enables a workflow with one single specimen acquisition instead of double image acquisition of the complete specimen area with different contrast methods. This leads to a significant time saving. (II) automated workflow for acquisition of single channel images mainly for oil analysis. (III) automated workflow for component cleanliness testing with AI-based object classification. Contains trainable, pre-trained models for AI- based analysis of multichannel images with different polarization directions. Training of the delivered machine learning models requires ZEN Toolkit AI . (IV) light microscope workflow for correlative technical cleanliness analysis (component and oil cleanliness) with automated and/or manual particle selection for subsequent SEM/EDS analysis in Free Mode. Requires in addition ZEN Toolkit Connect. (V) light microscope workflow for correlative component cleanliness analysis with AI-based object classification. Contains trainable, pre- trained models for AI-based analysis of multichannel images with different polarization directions. Supports automated and/or manual particle selection for subsequent SEM/EDS analysis in Free Mode. Training of the delivered machine learning models requires ZEN Toolkit AI. Requires in addition ZEN Toolkit Connect. The job templates cover image acquisition, image analysis and image processing, result presentation in interactive views for fast inspection and revision of detected particles, reports with results of characteristic values and standard specific methods per tested specimen. Automated storage of generated results and inspection views in the data archive. Inspection and export of archived data. (VI) post analysis workflow from archived data for calculation and interpretation of results to monitor air particles using particle traps supporting VDA 19.2 The job templates cover image acquisition, image analysis and image processing, result presentation in interactive views for fast inspection and revision of detected particles, reports with results of characteristic values and standard specific methods per tested specimen. Automated storage of generated results and inspection views in the data archive. Inspection and export of archived data.

ZEN Toolkit Motorized Acquisition Acquire images and control motorized components in multi-dimensional experiments. This package allows to acquire tiles, multi-position and z-stack images using motorized components. These can be combined with the included autofocus and direct processing. z-stack and enhanced depth of focus functionality: - definition of the Z-stack in "Start/Stop" and "Center" mode - size of the configurable Z-stack and number of possible Z-planes only limited by the travel range of the system and the minimum increment - "Optimal" button to automatically set the correct increment to satisfy the Nyquist criterion - integrated Z-drive backlash compensation for maximum precision and reproducibility - extraction of the sharp details from individual images at various focus positions and combination into an image with high depth of field Tiles <(>&<)> Positions - automatic scanning of predefined sample areas - acquisition of images according to a position list - support of focus correction maps, stitching and shading correction - compatible with software autofocus Software Autofocus: - determination of the optimal focus position in the sample - the autofocus works with transmitted, reflected and fluorescence light - definition of an autofocus region in the camera image

Dust protection Set L consisting of dust cover (L670xW460xH775 mm), eyepiece cover and protection cap for luminous-field diaphragm