Axio Imager scan Standard

Axio Imager.Z2 microscope stand with Z-drive mot. and TFT monitor - heavy duty focusing drive motorized, stepsize 10 nm, with fine drive knob and fine drive disk, flat with scale, changeable - Light Control mot. - main controller incl. USB-A, USB-C and TCP/IP interface - transmitted-light illumination mot. - external power unit 12V DC 100W, stabilized, 100V...240V AC/50...60Hz/230VA, - USB cable and country-specific line cable

Fine drive disk, flat with scala for Axio Imager 2, changeable

Camera path deflection on the left side, interface 60N slider with 2 switching positions, pos. 1 = 100% vis, pos. 2 for the optional mounting of path-deflecting mirror 100% = 100% doc or beam splitter 50% = 50% vis : 50% doc

Path deflecting mirror 100% for camera deflection, 34x46x4 mm

6-position objective nosepiece, HD DIC M27 mot.

Stage carrier, detachable with condenser carrier for Axio Imager suitable for sample heights from 0 mm up to 25 mm

6-position reflector turret mot. for P&C modules

Docking station for TFT-Display upgrading module for the TFT-Display of motorized stands Axio Imager and Axio Observer - free positioning on desktop - Control of z-drive and circular operation key unit - mechanical interface to attach Electronic Coaxial Drive - TFT-Display 30°-45° tiltable

Illuminator VIS-LED for mounting on the transmitted-light, reflected-light illuminator, included LED lamp module for VIS-LED - color temperature 5600 K - average lifetime > 10,000 hours - control signals: Trigger-input (BNC) for controlling, max. trigger frequency ca. 100 Hz - usable for objectives from 2.5x - incl. neutral-density filter 0.12 D=25x2 for use with digital microscopy cameras

Reflected-light illuminator for FL and HD for Axio Imager with shutter and with openings for manual stop sliders and filter wheels. Transmission range 340-1100 nm. With 340 nm and 1100 nm ~40%.

Shutter for transmitted-light illumination switching time < 200 ms switching frequency max. 2 Hz

Stop slider man. with centerable luminous-field diaphragm slider stop position can be set

Stop slider with centerable aperture stop

Reflector module FL EC P&C Reflection avoided through tilted mount for emission filters, max. filter thickness: 5 mm

Solid-State Light Source Colibri 7, Type R[G/Y]B-UV 5-channel fluorescence light source with integrated control unit for continuous brightness adjustment, quickly switchable and stable on the long term. With integrated monitor diode for repetitious brightness adjustment. Equipped with 5 excitation lines. Red (630nm) for excitation of Cy5, Alexa 631, TOTO-3 and similar dyes Yellow (590nm) for excitation of mCherry, Alexa 568, mPlum and similar dyes Green (555nm) for excitation of Cy3, TRITC, DsRed and similar dyes Blue (475nm) for excitation of eGFP, Fluo4, FITC and similar dyes UV (385nm) for excitation of DAPI, Alexa 405, Hoechst 33258 and similar dyes A country-specific power cable and a desktop power supply are included in delivery.

Scanning Stage 225x85; PIEZO (D) for Axio Imager and SteREO - with linear encoders and piezo drive - travel range 225 mm x 85 mm - max speed 100 mm/s - resolution: 0.1 µm - reproducibility: +/- 0.6 µm - absolute accuracy: +/- 3 µm - for mounting frames 313 x 148

Mounting frame for 8 slides 76x26 mm (D) - frame size 313x148

Binocular phototube 15°/25 (100:0/0:100), upright image adjustable stop, camera port with interface 60N

Filter Set 96 HE BFP shift free (E) EX BP 390/40, BS FT 420, EM BP 450/40

Filter set 90 HE LED (E) for use with the illumination system Colibri 5/7. Suitable for fluorescent dyes like DAPI, FITC, TRITC and Cy5 with excitation wavelengths 385, 475, 555 and 630 nm. Contains beam splitter QBS 405 + 493 + 575 + 653 and emission filter QBP 425/30 + 514/30 + 592/25 + 709/100.

Filter set 38 HE eGFP shift free (E) EX BP 470/40, BS FT 495, EM BP 525/50

Filter set 43 HE Cy 3 shift free (E) EX BP 550/25, BS FT 570, EM BP 605/70

Filter set 64 HE mPlum shift free (E) EX BP 587/25, BS FT 605, EM BP 647/70

Camera Adapter 60N-C 1" 1.0x

Eyepiece PL 10x/23 Br. foc.

Eyepiece eyecup

Objective EC Plan-Neofluar 5x/0.16 M27 (FWD=18.5mm)

Objective Plan-Apochromat 40x/0.95 Corr M27 (FWD=0.25mm), incl. Cover glasses, high performance, CG=0.17mm, box with 100 pc.

Objective Plan-Apochromat 20x/0.8 M27 (FWD=0.55mm), incl. Cover glasses, high performance, CG=0.17mm, box with 100 pc Lead-free

Universal condenser achromatic-aplanatic 0.9 H D Ph DIC, mot. with motorized front lens, aperture diaphragm and turret disk, with centerable Ph1, Ph2, Ph3 and D stops, already integrated, 3 mounts for DIC condenser modules, 1 separate position for bright field. For objective magnifications 1.0x-100x, FWD=1.2mm

Stage controller XY PIEZO WSB CAN (D) for scanning stages with PIEZO drive Interfaces: CAN, USB, RS232, Trigger IN, Trigger OUT incl. USB cable, CAN cable and stage cable 90 - 240 V / 40 - 60 Hz; 250 VA, country specific power cord

Joystick XY; CAN (D) for mechanical stages mot; CAN, scanning stages; CAN and stage controller XY STEP SMC 2009 - 2 speed ranges selectable - direct cable connection to CAN stage or to stage controller XY STEP SMC 2009

Extension cable for stage control 1.6 m (D) for usage with joystick, trackball or coaxial drive. Recommended for adaptation of cable length when a docking station is used.

System Configuration 2 for HP Z8 G5 (O) Installation of Windows 10 IoT Enterprise LTSC 2021 x64 incl. Windows 10 License - with ZEN software installation and system integration of respective system - for Celldiscoverer 7, LSM 900, LSM 980, Elyra 7, Lattice SIM, Axioscan 7, Lattice Lightsheet - only for 410203-9914-000 Microscopy Workstation Premium HP Z8 Fury G5

Monitor TFT 27" HP Z27q G3 (68 cm) (O) Visible diagonal: 68 cm, 16:9 Maximum resolution: 2560 x 1440 pixel @ 60 Hz Brightness: 350 nits Contrast: 1000:1 Viewing angle: horizontal 178 degree, vertical 178 degree Video input: DisplayPort 1.4, HDMI 2.0 Data connectors: 1 x USB Type B (upstream), 4 x SuperSpeed USB Type-A 5Gbps Power consumption: Maximum 69 W, Typical 35 W, Standby 0.5 W Dimensions incl. stand (H x W x D): 53.0 cm x 61.2 cm x 20.5 cm Weight incl. stand: 5.8 kg Ergonomic features: Tilt: -5°to +20°, Swivel: -45° to +45°, Height adjustable 15 cm, Pivot rotation: -90° to +90° Certification and compliance: CE, CB, GS, TÜV, FCC, TCO Operating voltage: 100-240 V, country-specific power cable

Language Pack English US (O) - Keyboard with English (US) layout

Network Adapter 2 x 10 GbE RJ45 (HP Z8 G5) (O) - Additional network adapter 2 x 10 GbE RJ45 (HP Z8 G5) e.g. for connection of storage systems - only for 410203-9914-000 Microscopy Workstation Premium HP Z8 Fury G5

Graphics Card NVIDIA RTX A4000 16 GB DP (O) Professional graphics card for Microscopy Workstation - 16 GB GDDR6 memory - PCI Express 4.0 Interface x16 - Hardware acceleration of DirectX 12.07, OpenGL 4.68 and CUDA - Support for up to four displays - Display outputs: 4 x DisplayPort 1.4a (DP) - DisplayPort support for 4 x 32" Display (3840 x 2160 Pixel)

Memory 32 GB (1x32) DDR5 4800 MHz ECC reg RAM (HP Z8 G5) (O) consisting of 1 x 32 GB module - DDR5 4800 MHz ECC registered DIMM - can be extended to 64 GB (2 x 410303-3206-000), 128 GB (4 x 410303- 3206-000) or 256 GB (8 x 410303-3206-000) - only for 410203-9914-000 Microscopy Workstation Premium HP Z8 Fury G5

Processor Intel Xeon W5-3425 (HP Z8 G5) (O) Default processor configuration - Intel Xeon W5-3425 (3.2 GHz, 30 MB cache, 12 cores) - only for 410203-9914-000 Microscopy Workstation Premium HP Z8 Fury G5

Microscopy Workstation Premium (HP Z8 G5) (O) HP Z8 Fury G5 Workstation - Chipset: Intel W790 - Memory Expansion Slots: 16 x DDR5, RDIMM, ECC, 4800 MHz Modules - Memory: max. 1024 GB RAM - PCI Express Connectors: 2 x PCI Express Generation5 x16 1 x PCI Express Generation 5 x4 2 x PCI Express Generation 4 x16 2 x PCI Express Generation 4 x4 1 x PCI Express Generation 3 x8 - SSD: 1 x 2 TB M.2 NVMe - Hard Drives: 2 x 12 TB SATA 7200 rpm (configured as 10 TB RAID 1hard drive) - Network Controller: 2 x GbE LAN interface (RJ 45) - USB: 2 x USB 3.2 Gen2x2 type C (front), 8 x USB 3.1 Gen 1 type A (2 x front, 6 x rear) - Certifications <(>&<)> Declarations: CE, CSA, UL, CCC, KCC - Power Supply: 100 - 240 V / 50 - 60 Hz / 1450 W with country specific plug - Chassis Dimensions: (H x W x D): 445 x 216 x 555 mm - USB Mouse, Recovery kit

30m Patch Cable CAT7

Trigger Cable Set Axiocam 5xx/7xx/8xx (D) for direct shutter synchronization using trigger board with signal distribution box, Colibri or HXP 120 V

Digital Microscopy Camera Axiocam 305 color R2 (D) Microscopy camera incl. Driver software 64bit, USB 3.0 PCIe x1 interface, USB 3.0 cable 3 m and IR barrier filter Hoya C5000 (coated) Number of Pixels: 2464 (H) x 2056 (V) = 5.07 Mega pixel color Sensor type: SONY IMX264 Exmor Pregius Global Shutter CMOS Pixel size: 3.45 μm x 3.45 μm Chip size: 8.5 mm x 7.1 mm, equivalent to 2/3" (diagonal 11.1 mm) Spectral range: With IR barrier filter app. 400 nm to 720 nm Full Well Capacity: Approx. 10,500 e Selectable Resolution: H x V Binning Factor 2464 x 2056 1x1 Basic Resolution 1232 x 1028 2x2 816 x 684 3x3 608 x 514 4x4 480 x 410 5x5 H x V Subsampling Factor 2464 x 2056 1x1 Basic Resolution 1232 x 1028 2x2 Live frame rates (depending on hardware and software configuration): H x V Frame Rate@1ms 2464 x 2056 36 fps 1232 x 1028 88 fps (2x2 Subsampling) Frame rates for time series recording (depending on computer hardware and software configuration) H x V (ROI) FrameRate 2464 x 2056 36 fps 2048 x 2048 36 fps 1920 x 1080 67 fps 1024 x 1024 70 fps 512 x 512 136 fps 256 x 256 255 fps 128 x 128 456 fps Readout of Sensor Sub-Regions ("ROI"): Adjustable Digitization: 8 and 12 Bit / Pixel Dynamic Range: Typical > 4800 : 1 (>73 dB) at typ. 2.2 e readout noise Dark current: <(><<)> 1.0 e/p/s @ 25°C Integration Time: 0.1 ms to 4 s Cooling: Temperature stable @ 25°C for ambient temperatures between 18°C and 30°C Status-LED for Camera: color coded operation status, dimmable Interface (camera): USB 3.0 interface Optional operation with USB 2.0 connection at reduced frame rate Optical Interface: C-Mount Thread depth for objectives: max. 5 mm Size / Weight: approx. App. 10.8 cm x 7.8 cm x 4.3 cm (2.3"x3.2"x1.7") / 580 g Housing: blue anodized aluminum,with cooling fins on top, 1/4" photo thread for tripod mount Registration: CE Power Supply: Max. 4W, power supply provided by USB 3.0 bus for camera electronics Environmental conditions: 5° … +35° Celsius, max 80% relative air humidity, not condensing, free air circulation required Supported Operating Systems: for ZEN: Windows 10 x64 Supported Application Software: ZEN 3.6 (blue edition, Hotfix 3.6.6) or higher, ZEN core 3.5 (Hotfix 3.5.4) or higher The required application software is available via the ZEISS Download Center.

Microscopy Camera Axiocam 807 mono (D) High performance microscopy monochrome camera incl. driver software 64bit, Interface card USB 3.1 Gen2 PCIe x4, dual USB 3.0 cable 3 m Sensor Type: CMOS, monochrome, Global Shutter, Sony IMX420 Number of Pixels: 3216 (H) x 2208 (V) = 7.1 Mega Pixels Pixel Size: 4.5 µm x 4.5 µm Chip Size: 14.5 mm x 9.9 mm, equivalent to 1.1" (17,6 mm diagonal) Spectral Range: With protection glass, 350 nm to 1000 nm Peak QE: 78% @ 520 nm (typ.) Full Well Capacity: Approx. 25,000 e- at gain 1x (typ.) Adjustable Signal Amplification: 1x, 2x, 4x, 8x, 16x Selectable Resolution: H x V Binning Factor (digital) 3216 x 2208 1x1 basic pixel count 1608 x 11042x2 1072 x 991 3x3 884 x 743 4x4 643 x 594 5x5 (at 2x2 combined analog and digital binning for further increase of sensitivity) H x V Subsampling Factor (full field of view) 3216x2208 1x1 1608 x 1104 2x2 Live frame rates (depending on hardware and software configuration): Exp. Time 1ms H x V Frame Rate 3216 x 2208 >30 fps (High Res mode) 1602 x 1104>30 fps (Fast Mode, reduced CPU load) Frame rates for time series recording (1 ms exposure, further depending on sensor ROI, hardware and software configuration) H x V (ROI) Frame Rate (fps) 3216 x 2208 73 1929 x 1080 145 1608 x 1104 260 (2x2 subsampling; full field of view) 1024 x 1024 151 512 x 512 282 1920 x 256 487 Read Noise Full Well Capacity Dynamic Range (at gain) 5.7 e (1x) 25,000 e 1:4420 4.6 e (2x) 12,500 e 1:2730 3.9 e (4x) 6,250 e 1:1610 3.4 e (8x) 3,125 e 1: 930 2.9 e (16x) 1,500 e 1: 530 Low Noise Mode: 4.0 e 25,000 e 1:25,000 SoftwareSupported Camera Operation: Exposure time adjustment with continuous, automatic or manual mode, Adjustable over or under-exposure mode, Optional measurement on defined target area (spot-meter), Image sharpening, Dark current compensation, Single pixel noise correction Readout of Sensor Sub Regions ("ROI"): Adjustable Digitization: 8, 12, 14 bit / pixel (8 out of 14 bit or compression mode) Dark Current: <(><<)> 0.3 e/p/s @ 25 °C sensor temperature Cooling: Stabilized temperature to 25 °C (mounted on microscope, free air flow, ambient temp. 22 °C) Exposure Time Range: 0.1 ms to 60 s Hardware Trigger: Trigger-In for exposure time control Trigger-Out for exposure time Readout time and trigger ready status (further accessories required) Status-LED for Camera: Color coded operation status, dimmable Camera Interface: Dual USB 3.0 SuperSpeed,2 x 5 Gbit/s for data and power supply (optional single USB 3.0 at lower frame rates and without cooling) Optical Interface: C-Mount Thread Depth for Objectives: Max. 5mm Size / Weight: Approx. 10.8 cm x 7.8 cm x 6.1 cm (4.2"x 3.1" x 2.4")/ 620 g Housing: Blue anodized aluminum, with cooling fins on top 1/4" photo thread for tripod mount Registration: CE Power Supply: Provided by Dual USB 3.0, 5V, max. 7 W powerconsumption Environmental Conditions: 5 °C to 35 °C, max.80% relative air humidity, not condensing, free air circulation required Supported Operating Systems: for ZEN: Windows 10 x64 Enterprise Supported Application Software: ZEN 3.6 (blue edition) or higher, ZEN 3.5 core or higher The required application software is provided via ZEISS Microscopy Installer or ZEISS Download Center.

ZEN 3.9 Image acquisition and processing under Windows 10 x64 User interface configurable, control of the ZEISS microscope systems and components, extensive acquisition and analysis, CZI image format The following functions are included: - arivis cloud advanced - Basic 3D viewing - Colocalization - Connect basic - Direct processing - Manual EDF - Measurement - Panorama - Spectral unmixing - ZEN Data Storage Client - Acquisition Base (time series and multichannel)

ZEN Toolkit Motorized Acquisition Acquire images and control motorized components in multi-dimensional experiments. This package allows to acquire tiles, multi-position and z-stack images using motorized components. These can be combined with the included autofocus and direct processing. z-stack and enhanced depth of focus functionality: - definition of the Z-stack in "Start/Stop" and "Center" mode - size of the configurable Z-stack and number of possible Z-planes only limited by the travel range of the system and the minimum increment - "Optimal" button to automatically set the correct increment to satisfy the Nyquist criterion - integrated Z-drive backlash compensation for maximum precision and reproducibility - extraction of the sharp details from individual images at various focus positions and combination into an image with high depth of field Tiles <(>&<)> Positions - automatic scanning of predefined sample areas - acquisition of images according to a position list - support of focus correction maps, stitching and shading correction - compatible with software autofocus Software Autofocus: - determination of the optimal focus position in the sample - the autofocus works with transmitted, reflected and fluorescence light - definition of an autofocus region in the camera image

ZEN Toolkit Bio Apps Easy-to-use image analysis for standard life science applications. Choose from the selection of Bio Apps one, that fits your application and simply adapt the preconfigured Bio App to your data if necessary. Use fast, classical segmentation methods or powerful AI models to detect your cells even more accurately. This toolkit is "AI-ready", meaning you can use previously trained AI models in the Bio Apps without needing another license. Simply load an AI model trained with the ZEN Toolkit AI or a DNN trained on apeer for semantic or instance segmentation in the BioApp. After analysis, the segmented images are displayed along with customized results tables and diagrams (e.g., heatmaps and scatterplots) so you can get a quick overview of your sample. At the same time, tables, charts, and images are interlinked so that you can explore the results interactively. All Bio Apps offer a heatmap view, making them particularly suitable for analyzing experiments in multi-well plates. They support both 2D endpoint and live cell assays. - Get started fast with Bio Apps tailored to specific applications - Available Bio Apps: Cell counting, confluency, gene- and protein expression and automated spot detection - Easy operation with predefined parameters - Choose between fast classical segmentation or more powerful AI-models: use AI models trained with the ZEN Toolkit AI or DNNs trained on apeer with semantic or instance* segmentation (*not Confluency) - Visualize your results with dedicated and interactive result displays (table, heatmap, timeseries, xy-scatterplot, histogram) - Export your results, plots, and processing settings - Supports 2D fixed timepoint and time-series analysis Requirements: - The use of AI semantic segmentation requires an installation of Z Zeiss Python. - The use of AI instance segmentation requires an installation of Docker Desktop For the use of AI models the recommended hardware configuration is 8GB GPU and 64GB RAM. Only CPU and Nvidia GPUs are supported.

Dust protection Set L consisting of dust cover (L670xW460xH775 mm), eyepiece cover and protection cap for luminous-field diaphragm