Axio Imager.A2 complete system for Correlative Microscopy

Axio Imager.A2 microscope stand - Z-drive man., with fine drive knob and fine drive disk, flat with scala, changeable - stage/condenser carrier, detachable - transmitted-light illumination man. - double filter wheel, discrete, man. with neutral-density filter set - Light Control man. with RS 232 interface - integrated 12V DC 100W power unit, stabilized, 100V...240V AC/50...60Hz/168VA - country-specific line cable and RS 232 cable

6-position objective nosepiece, HD DIC M27 cod.

6-position reflector turret man. cod. for P&C modules

Reflected-light illuminator for FL and HD for Axio Imager with shutter and with openings for manual stop sliders and filter wheels. Transmission range 340-1100 nm. With 340 nm and 1100 nm ~40%.

Antiglare screen

Stop slider man. with centerable luminous-field diaphragm slider stop position can be set

Stop slider with centerable aperture stop

Reflector module FL EC P&C Reflection avoided through tilted mount for emission filters, max. filter thickness: 5 mm

Illuminator microLED 2 for mounting on the lamp interface for transmitted-light or reflected-light illumination - colour temperature 5700 K - colour rendering index >90 - average lifetime >60000 hours - usable for objectives from 2.5x

Illumination adapter for light guide (D) Suitable for HXP 120 and X-Cite 120, coupling diameter of light guide 5 mm

Illuminator HXP 120 V (D) inclusive built in power supply, lamp module and infrared filter. Fluorescence light source with motorized brightness switch and integrated motorized shutter, that works stepper motor controlled, fast and vibration free. With CAN connection for switching the shutter and the motorized brightness switch via ZEN (blue edition) and ZEN 2 (black edition). Interfaces: - 1x CAN (CAN-HD15) - 1x Trigger in (BNC) - 1x Trigger out (BNC) - 1x EXT (BNC) for remote control unit The package includes light guide, cable BNC and cable CAN.

Shutter remote control for HXP 120 V incl. connecting cable 2 m. Usable as knuckle switch or pedal switch.

Converter CAN - USB Rev.2 (D) Interface for components with CAN to USB PC Sockets: 1x CAN, 1x USB, 24 V Included accessories: power supply 100 - 240 V with country specific plug, USB cable

DIC slider EC PN 10x/0.30 I, 20x/0.50 II, PA 20x/0.80 II

DIC slider EC PN 40x/0.75 II, EC PN 100x/1.30 III

Compensator mount 6x20

Analyzer module DIC ACR P&C shift free for transmitted light optimized for DIC in transmitted light, not suitable for polarization. Pol filter for imaging free from pixel shift, almost without wedge error.

Mechanical Stage 75x50 mot; CAN (D) for Axio Imager and SteREO - hard coating, linear encoders - stepper motor drive with integrated controller - connection via CAN bus - travel range 75 mm x 50 mm - max speed 100 mm/s - resolution: 0.1 µm - reproducibility: +/- 1 µm - absolute accuracy: +/- 5 µm - incl. CAN cable

Specimen holder CorrMic LS for cover glasses 22x22 mm (D) - dimension 76 mm x 26 mm - mounting for cover glasses 22 mm x 22 mm - 3 calibration reference points - incl. cover glasses high performance, D=0.17mm, 22 x 22 mm, box with 200 pc.

Specimen holder, special, for 2 slides 76x26 mm particularly for use with immersion objectives

Binocular Ergophototube 20°/23 (100:0/0:100), reversed image variable, continuous vertical adjustment 44 mm, camera port with interface 60N

Conversion filter 5700-3200 K, d=25 mm for adapting the LED light to the color impression of halogen light

Filter set 49 DAPI shift free (E) EX G 365, BS FT 395, EM BP 445/50

Filter set 20 Rhodamin shift free (F) EX BP 546/12, BS FT 560, EM BP 575-640

Filter set 38 Endow GFP shift free (E) EX BP 470/40, BS FT 495, EM BP 525/50

Camera Adapter T2-C 1'' 1.0x; adjustable (x,y,z and rotation)

Adapter 60N - T2 1.0x

Eyepiece PL 10x/23 Br. foc.

Eyepiece eyecup

Objective EC Plan-Neofluar 10x/0.3 M27 (FWD=5.2mm)

Objective EC Plan-Neofluar 20x/0.50 M27 (FWD=2.0mm)

Objective EC Plan-Neofluar 40x/0.75 M27 (FWD=0.71mm), incl. Cover glasses, high performance, CG=0.17mm, box with 100 pc.

Objective EC Plan-Neofluar 100x/1.30 Oil M27 (FWD=0.20mm), incl. Immersol 518 F, oiler 20ml and Cover glasses, high performance, CG=0.17mm, box with 100 pc.

Achromatic-aplanatic universal condenser 0.9 H D Ph DIC with switchable front lens, turret disk, with centerable Ph1, Ph2, Ph3 and D stops, already integrated, 3 mounts for DIC condenser modules, 1 separate position for bright field. For objective magnifications 1.0x-100x, WD=1.2mm

Condenser module DIC I/0.9 with polarizer

Condenser module DIC II/0.9 with polarizer

Condenser module DIC III/0.9 with polarizer

Joystick XY; CAN (D) for mechanical stages mot; CAN, scanning stages; CAN and stage controller XY STEP SMC 2009 - 2 speed ranges selectable - direct cable connection to CAN stage or to stage controller XY STEP SMC 2009

Microscopy Workstation Compact Core i5 multilingual (O) - HP Z240 Tower Workstation - CPU: Intel® CoreTM i5-6500 3.2 GHz 6 MB / 2133 MHz - Chipset: Intel® C236 - Memory: max. 64 GB RAM - Memory Expansion Slots: 4 x DDR3-RAM modules / 2133 MHz unbuffered, non-ECC - PCI Express Connectors: 1 x PCI Express Generation 3 x16 1 x PCI Express Generation 3 x4 (x16 mechanical) 1 x PCI Express Generation 3 x4 1 x PCI Express Generation 3 x1 1 M.2 slot (PCIe Gen3 x4) - Graphics interface onboard: Intel® HD Graphics 530 2 x Display Port 1.2 (max. 3840 x 2160 pixel), 1 x DVI-D single link (max. 1920 x 1200 pixel) - Hard Drives: 1 x 4 TB SATA 7200 rpm - Optical Storage: DVD +/- RW recorder for rewriteable media - Network Controller: Integrated 10/100/1000 LAN interface - I/O: 1 x serial RS232, 6 x USB 3.0 (2 x front, 4 x rear), 4 x USB 2.0 (2 x front, 2 x rear), PS/2 for keyboard and mouse - Certifications & Declarations: CE, CSA, UL, FCC - Power Supply: 100 - 240 V / 50 - 60 Hz / 400 W with country specific plug - Chassis Dimensions: (H x W x D): 399 x 170 x 442 mm, approx. 10 kg - Rescue kit

Language Package Windows 7 Ultimate Embedded x64 English US (O) - Windows 7 Ultimate Embedded SP1 x64 operating system - Keyboard with english (US) layout

Monitor TFT 24'' HP Z24n G2 (61 cm) (O) Visible diagonal: 60.96 cm, 16:10 Max. resolution: 1920 x 1200 pixel @ 60 Hz Brightness: 300 cd/m2 Contrast: 1000:1 Viewing angle: horizontal 178 degree, vertical 178 degree 3 x Video input: DisplayPort 1.2, DVI-D, HDMI 1.4 3 x USB 3.0, 2 x USB 3.1 Type-C Power consumption: Maximum 92 W, Typical 40 W, Power Saving 0.5 W Dimensions with stand (H x W x D): 50.3 cm x 53.2 cm x 21.4 cm Weight: 7.2 kg (incl. stand) Ergonomic features: Tilt: -5°to +22°, swivel: -45° to +45°, height adjustable 15 cm, Pivot rotation 90° Certification and compliance: CE, CSA, GS, TÜV, FCC, TCO Operating voltage: 100-240 V, country-specific power cable

Memory 16 GB (1x16) DDR4-2133 MHz non-ECC unbuffered RAM (O) consisting of 1 x 16 GB module

Microscopy Camera Axiocam 503 mono (D) High Performance microscopy camera incl. driver software 64bit, USB 3.0 PCIe x1 interface, dual USB 3.0/USB 2.0 cable 3 m and BK7 protection glass (coated) Number of Pixels: 1936 (H) x 1460 (V) = 2.8 Mega Pixels Pixel size: 4.54 µm x 4.54 µm Chip size: 8.8 mm x 6.6 mm, equivalent to 2/3'' (11 mm diagonal) Spectral range: With protection glass app. 400 nm to 1000 nm Max. Full Well Capacity: Approx. 15.000 e Selectable Resolution: H x V Binning Factor 1936 x 1460 1x1 Basic Resolution 968 x 728 2x2 640 x 484 3x3 480 x 364 4x4 384 x 292 5x5 Live frame rates (depending on hardware and software configuration): Exp. Time 10ms, color enhancement off, 39 Mhz pixel clock, Quadport Readout H x V Binning Factor Frame Rate 1936 x 1460 1 38 968 x 728 2 61 640 x 484 3 76 Frame rates for time series recording Continuous Mode with 39 Mhz pixel clock and QuadPort Readout (depending on computer hardware and software configuration) H x V Binning Factor Frame Rate 1936 x 1460 1 38 968 x 728 2 61 640 x 484 3 76 480 x 364 4 87 384 x 292 5 93 Timelapse recording with ''Burst Mode''-function Readout of Sensor Sub-Regions (''ROI''): Adjustable Digitization: 14 Bit / 39 MHz or 13 MHz pixel clock Dynamic Range: Typical >2500: 1 (>68 dB) at typ. <(><<)> 6.5 e readout noise Dark current: ca. 0.06 e/p/s Integration Time: 1 ms to 60 s Cooling: One stage Peltier cooling, regulated to 18°C sensor temperature, Additional digital dark current compensation, Eight pre-loadable sets of imaging parameters for speed optimized multi modal image acquisition, acquisition Time Stamp with image data, Status-LED for Camera: color coded operation status, dimmable, Status-LED for Trigger: color coded trigger status, dimmable, I/O Control signals: galvanic isolated I/O signals for exposure time, readout time, trigger ready, (i.e. for controlling external mechanical shutters), one trigger input for exposure control, 5V auxiliary voltage, GND Interface: USB 3.0, 240 MBytes/s, 3 m double-USB cable with USB 3.0/USB 2.0, Optional operation with USB 2.0 connection at reduced frame rate, Optional operation without cooling for use at short exposure times with reduced power consumption, Optical Interface: C-Mount, Teflon coated thread Thread depth for objectives: max. 5 mm Max. file size per image: app. 5.6 MB at 1936 x 1460 pixels at 14 bit/pixel Size / Weight: app. 10.8 cm x 7.8 cm x 4.3 cm (2.3''x3.2''x1.7'') / 500 g Housing: blue anodized aluminum, with cooling fins, 1/4'' photo thread for tripod mount Registration: CE, Power Supply: power supply provided by USB 3.0 bus for camera electronics and USB 2.0 for Peltier-cooling, Power consumption: 7W (5V, 1.4A) Environmental conditions: 5° ... +35° Celsius, max 80% relative air humidity, not condensing, free air circulation required Operating Systems: Windows 10 Enterprise embedded x64 and older Windows x64 systems

ZEN 2.6 pro Hardware License Key Image acquisition and processing under Win 7 / 10 x64. User interface configurable, control of the ZEISS microscope systems and components, extensive acquisition and analysis. CZI image format. The following modules are included: - ZEN Module Measurement - ZEN Module Multi Channel - ZEN Module Panorama - ZEN Module Manual Extended Focus - ZEN Module Connect Entry

ZEN Module Shuttle & Find Module for acquisition and correlation of light and electron microscope images - Calibration of XY-position and image acquisition with light microscope - Transfer of images with position data to SEM and XB running Shuttle & Find module under ZEN software - Marking and easy repositioning of interesting sample regions in SEM/XB - Overlay of light and electron microscopic images

Dust protection Set L consisting of dust cover (L670xW460xH775 mm), eyepiece cover and protection cap for luminous-field diaphragm