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Objective LD A-Plan 32x/0.45 Ph1 M27

Objective LD A-Plan 32x/0.45 Ph1 M27

Item no.: 421261-9910-000
(no longer available)

Objective LD A-Plan 32x/0.45 Ph1 M27 (WD=3.1mm at D=1mm polystyrene)

Full Description

The objective is designed for the use of polystyrene (PS) cover glasses with thickness 1.0 mm.

Magnification 32x
Numerical Aperture 0.45
Free Working Distance [mm] 3.1
Coverglass Thickness [mm] 1.0
Thread Type M27x0.75
Immersion Without Immersion
Field of View [mm] 23
Parfocal Length [mm] 45.06
Long Distance Yes
Correction Ring
Optical System Infinity-Color-Corrected System (ICS)
Color Correction
Biomedical Applications
Fluorescence Yes
- Multichannel
- Ultraviolet Transmission
- Infra Red Transmission
BrightField Yes
DIC [Differential Interference Contrast]
High Contrast DIC
Polarization-Optical DIC
Phase Contrast Ph 1
VAREL Contrast
Hoffman Modulation Contrast
Polarization Contrast
Materials (Reflected Light) Applications
BrightField Yes
Reflected Light DIC
High Contrast DIC
DIC with circular polarized light
Total Interference Contrast
Polarization Contrast
Definite Focus.2
Confocal Microscopy
- Ultra Violet
- VIS (visible light)
- NLO-IR / 2 Photon
Total Internal Reflection Fluorescence
  • Coverglass cap for CG=0.17mm
    Coverglass cap for CG=0.17mm
    Item no.: 422904-9001-000
  • Objective ring ACR for objective sleeve cylindrical short
    Objective ring ACR for objective sleeve cylindrical short
    Item no.: 424508-0000-000
Mechanical Dimensions
Transmittance curve
LD A-Plan

The series with large working distance represent the entry level for inverted microscopy. They are corrected for cover glass and vessel bottom thickness up to 2 mm. This means optimal in the main applications of LD objectives, such as observation through thicker cell culture vessels etc. But also the use of thinner cover glasses for example in the range of 0.17 to 0.6 mm is easily possible with the simple use of a cover glass cap. The available contrast methods are ranging from brightfield, phase contrast to VAREL, Hoffman Contrast up to ?PlasDIC?, the Zeiss exclusive polarization optical differential interference contrast, that makes applications like ICSI now possible even in plastic vessels with high resolution and brilliant contrast. Of course they are also suitable for fluorescence applications with excitation wavelengths within the visible spectrum.

Objective LD A-Plan 32x/0.45 Ph1 M27 (WD=3.1mm at D=1mm polystyrene)