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    Objective LD A-Plan 10x/0.25 iHMC M27

    Objective LD A-Plan 10x/0.25 iHMC M27

    Item no.: 421241-9930-000
    Description

    Objective LD A-Plan 10x/0.25 iHMC M27 (FWD=8.4mm at CG=1mm polystyrene), for improved Hoffman Modula...

    Full Description

    The objective is designed for the use of polystyrene (PS) cover glasses with thickness 1.0 mm.

    Magnification 10x
    Numerical Aperture 0.25
    Free Working Distance [mm] 8.4
    Coverglass Thickness [mm] 1.0
    Thread Type M27x0.75
    Immersion Without Immersion
    Field of View [mm] 23
    Parfocal Length [mm] 45.06
    Long Distance Yes
    Correction Ring
    Iris
    Optical System Infinity-Color-Corrected System (ICS)
    Flatness
    Color Correction
    Biomedical Applications
    Fluorescence Yes
    - Multichannel
    - Ultraviolet Transmission
    - Infra Red Transmission
    BrightField Yes
    Differential Interference Contrast
    High Contrast DIC
    Polarization-Optical DIC
    Phase Contrast
    VAREL Contrast
    Hoffman Modulation Contrast Yes
    Polarization Contrast
    Materials (Reflected Light) Applications
    BrightField Yes
    BrightField/DarkField
    Reflected Light DIC
    High Contrast DIC
    DIC with circular polarized light
    Total Interference Contrast
    Polarization Contrast
    Options
    Definite Focus.2
    Confocal Microscopy
    - Ultra Violet
    - VIS (visible light)
    - NLO-IR / 2 Photon
    Total Internal Reflection Fluorescence
    ApoTome
    Microdissection
    • Objective ring ACR for objective sleeve cylindrical short
      Objective ring ACR for objective sleeve cylindrical short
      Item no.: 424508-0000-000
    Mechanical Dimensions
    Transmittance curve
    LD A-Plan

    The series with large working distance represent the entry level for inverted microscopy. They are corrected for cover glass and vessel bottom thickness up to 2 mm. This means optimal in the main applications of LD objectives, such as observation through thicker cell culture vessels etc. But also the use of thinner cover glasses for example in the range of 0.17 to 0.6 mm is easily possible with the simple use of a cover glass cap. The available contrast methods are ranging from brightfield, phase contrast to VAREL, Hoffman Contrast up to “PlasDIC”, the Zeiss exclusive polarization optical differential interference contrast, that makes applications like ICSI now possible even in plastic vessels with high resolution and brilliant contrast. Of course they are also suitable for fluorescence applications with excitation wavelengths within the visible spectrum.

    Objective LD A-Plan 10x/0.25 iHMC M27 (FWD=8.4mm at CG=1mm polystyrene), for improved Hoffman Modulation Contrast