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    Objective W

    Objective W "N-Achroplan" 20x/0.5 M27

    Item no.: 420957-9900-000

    Objective W N-Achroplan 20x/0.5 M27 (FWD=2.6mm)

    Full Description

    This objective is designed for dipping into physiologic salt solution.

    Magnification 20x
    Numerical Aperture 0.5
    Free Working Distance [mm] 2.6
    Coverglass Thickness [mm] 0
    Thread Type M27x0.75
    Immersion Water
    Field of View [mm] 23
    Parfocal Length [mm] 45.00
    Long Distance
    Correction Ring
    Optical System Infinity Color Corrected System (ICS)
    Color Correction
    Biomedical Applications
    Fluorescence Yes
    - Multichannel
    - Ultraviolet Transmission
    - Infra Red Transmission
    BrightField Yes
    Differential Interference Contrast
    High Contrast DIC
    Polarization-Optical DIC
    Phase Contrast
    VAREL Contrast
    Hoffman Modulation Contrast
    Polarization Contrast
    Materials (Reflected Light) Applications
    BrightField Yes
    Reflected Light DIC
    High Contrast DIC
    DIC with circular polarized light
    Total Interference Contrast
    Polarization Contrast
    Definite Focus.2
    Confocal Microscopy Yes
    - Ultra Violet
    - VIS (visible light)
    - NLO-IR / 2 Photon
    Total Internal Reflection Fluorescence
    • DIC slider LD PN 20x/0.40 II
      DIC slider LD PN 20x/0.40 II
      Item no.: 426954-0000-000
    • Objective ring ACR for objective sleeve cylindrical short
      Objective ring ACR for objective sleeve cylindrical short
      Item no.: 424508-0000-000
    • Box of lens cleaning paper
      Box of lens cleaning paper
      Item no.: 462975-0000-000
    • Adapter 30mm with optics and DIC slider mount
      Adapter 30mm with optics and DIC slider mount
      Item no.: 424516-9041-000
    • Immersion medium
      Immersion medium "Immersol" W 2010, oiler 20 ml
      Item no.: 444969-0000-000
    Mechanical Dimensions
    Transmittance curve
    W Achroplan/W N-Achroplan

    These water immersion objectives are used especially in physiology, in conjunction with the upright microscope, for samples such as thick brain sections. On account of their thickness, such samples cannot be observed with inverted “Axiovert” microscopes and LD objectives, contrary to the examination of cell cultures in transparent vessels. The upright “Axioskop” 2 FS makes it possible to immerse the objective into a medium contained, e.g., in a Petri dish and examine the specimen from above. Focusing of the “Axioskop” 2 FS acts on the nosepiece instead of the stage (FS = Fixed Stage).These objectives are, of course, also used for transmitted-light microscopy. One example is the draining of electrical potential from cells. “Achroplan” water immersion objectives are specially adapted to requirements in electrophysiology (patch-clamp technique), in intravital microscopy, in microcirculation studies, in the examination of vital brain sections and other thick specimens. In these techniques, some fundamental conditions have to be satisfied: Superb optical performance and transmission, slender form, front part of special material and long working distance. All Water “Achroplan” objectives have fields flattened across a diameter of 20 mm. They allow all contrasting methods, including high-resolution differential interference contrast (DIC). Thanks to their high numerical apertures, they not only provide excellent images but also are ideal for fluorescence - not least because of the use of special glass types that are highly transmissive to the excitation light. Further, these objectives are suitable for the "INFRAPATCH" method at wavelengths up to 780 nm (special IR versions are available). The slender front part of the objective and the long working distance of these objectives provide sufficient space for the specimen to be well accessible to all microtools (patch-clamp electrodes, microinjection capillaries etc.). It is possible to implement big positioning angles to minimize self-capacitance in patch-clamp potential draining. Moreover, the slender front part barrel is made of special, insulating plastics of extremely low self-capacitance. The material is inert, i.e. it does not release metal ions to the surrounding medium; it is corrosion-proof, chemically resistant and of minimum thermal conductivity.

    Objective W N-Achroplan 20x/0.5 M27 (FWD=2.6mm)