Objective LD LCI Plan-Apochromat 63x/1.2 Imm Corr DIC M27

Objective LD LCI Plan-Apochromat 63x/1.2 Imm Corr DIC M27

Item no.: 420882-9870-000
Description

Objective LD LCI Plan-Apochromat 63x/1.2 Imm Corr DIC M27 for immersion media with refractive index ...

Full Description

The rating of the option Definite Focus.2 is valid for use with glycerine immersion.

Magnification 63x
Numerical Aperture 1.2
Free Working Distance [mm] 0.49 at cover glass 0.17
Coverglass Thickness [mm] 0.15 - 0.19
Thread Type M27x0.75
Immersion Glycerine and Oil
Field of View [mm] 18
Parfocal Length [mm] 45.06
Long Distance Yes
Correction Ring Yes
Iris
Optical System Infinity Color Corrected System (ICS)
Flatness
Color Correction
Biomedical Applications
Fluorescence Yes
- Multichannel
- Ultraviolet Transmission
- Infra Red Transmission
BrightField Yes
Differential Interference Contrast
High Contrast DIC
Polarization-Optical DIC
Phase Contrast
VAREL Contrast
Hoffman Modulation Contrast
Polarization Contrast
Materials (Reflected Light) Applications
BrightField Yes
BrightField/DarkField
Reflected Light DIC
High Contrast DIC
DIC with circular polarized light
Total Interference Contrast
Polarization Contrast
Options
Definite Focus.2
Confocal Microscopy Yes
- Ultra Violet
- VIS (visible light)
- NLO-IR / 2 Photon
Total Internal Reflection Fluorescence
ApoTome
Microdissection
  • DIC slider LD LCI PApo 63x/1.2 III
    DIC slider LD LCI PApo 63x/1.2 III
    Item no.: 426947-9010-000
  • Cover glasses, high performance, D=0.17mm, box with 1000 pc.
    Cover glasses, high performance, D=0.17mm, box with 1000 pc.
    Item no.: 474030-9000-000
  • Objective ring ACR for objective sleeve conical long
    Objective ring ACR for objective sleeve conical long
    Item no.: 424510-0000-000
  • Immersion medium Immersol G, oiler 20 ml
    Immersion medium Immersol G, oiler 20 ml
    Item no.: 462959-9901-000
  • Immersion oil
    Immersion oil "Immersol" 518 F fluorescence free, bottle 100 ml
    Item no.: 444962-0000-000
  • Immersion oil
    Immersion oil "Immersol" 518 F fluorescence free, bottle 500 ml
    Item no.: 444964-0000-000
  • Immersion oil
    Immersion oil "Immersol" 518 F fluorescence free, oiler 20 ml
    Item no.: 444960-0000-000
  • Immersion oil Immersol 518 F for 30°C fluorescence free, oiler 20ml
    Immersion oil Immersol 518 F for 30°C fluorescence free, oiler 20ml
    Item no.: 444970-9000-000
  • Immersion oil Immersol 518 F for 37°C fluorescence free, oiler 20ml
    Immersion oil Immersol 518 F for 37°C fluorescence free, oiler 20ml
    Item no.: 444970-9010-000
  • Box of lens cleaning paper
    Box of lens cleaning paper
    Item no.: 462975-0000-000
Mechanical Dimensions
Transmittance curve
LCI Plan-Apochromat

These objectives have been specifically designed for Life Cell Imaging techniques and are designed for the temperature interval between 23° and 37°C. The 25x/0.8 can be used with the immersion mediums water, glycerine and oil. A single correction collar permits simple and convenient compensation for spherical aberration caused, for example, by different cover slip thicknesses, temperature drift or immersion media with a different refractive index, adjustable between water and glycerine. For the examination of living objects, water is particularly suitable as immersion medium. Furthermore, cleaning is extremely easy. For critical fluorescence examinations, cleaned glycerol is the ideal immersion medium, as it has very low autofluorescence. Therefore, glycerol and water should be preferred for the microscopy of living objects, since these are found in a medium with a similar refractive index. The "Refractive Index Mismatch" indicates the deviation of the actually available refractive index from the one that the objective has been calculated for. This deviation too can be compensated for by means of the correction collar. Used with inverted microscopes, multi-immersion objectives are ideal for the limited compensation of non-standard glass bottom thicknesses (D ≠ 0,17) or the popular use of water as immersion medium with a slightly lower numerical aperture than oil-immersion objectives.
Special emphasis has been placed on good accessibility of the optical axis with micromanipulators.

Objective LD LCI Plan-Apochromat 63x/1.2 Imm Corr DIC M27 for immersion media with refractive index 1.43 or higher to glycerine or oil immersion. (UV) VIS-IR (CG=0.15-0.19mm) (FWD=0.49mm at CG=0.17mm) incl. Immersol G, bottle 20 ml and Cover glasses, high performance, CG=0.17mm, box with 100 pc.