Objective LD LCI Plan-Apochromat 40x/1.2 Imm autocorr DIC M27

Objective LD LCI Plan-Apochromat 40x/1.2 Imm autocorr DIC M27

Item no.: 420862-9970-790
Description

Objective LD LCI Plan-Apochromat 40x/1.2 Imm autocorr DIC M27 for water, silicon oil or glycerine im...

Full Description

The rating of the option Definite Focus.2 is valid for use with water immersion.

Magnification 40x
Numerical Aperture 1.2
Free Working Distance [mm] 0.41 at cover glass 0.17
Coverglass Thickness [mm] 0.15 - 0.19
Thread Type M27x0.75
Immersion Water, Silicone oil and Glycerine
Field of View [mm] 18
Parfocal Length [mm] 45.06
Long Distance Yes
Correction Ring Yes
Iris
Optical System Infinity Color Corrected System (ICS)
Flatness
Color Correction
Biomedical Applications
Fluorescence Yes
- Multichannel
- Ultraviolet Transmission
- Infra Red Transmission
BrightField Yes
Differential Interference Contrast
High Contrast DIC
Polarization-Optical DIC
Phase Contrast
VAREL Contrast
Hoffman Modulation Contrast
Polarization Contrast
Materials (Reflected Light) Applications
BrightField Yes
BrightField/DarkField
Reflected Light DIC
High Contrast DIC
DIC with circular polarized light
Total Interference Contrast
Polarization Contrast
Options
Definite Focus.2
Confocal Microscopy Yes
- Ultra Violet
- VIS (visible light)
- NLO-IR / 2 Photon
Total Internal Reflection Fluorescence
ApoTome
Microdissection
  • DIC slider LD LCI PApo 40x/1,2 III
    DIC slider LD LCI PApo 40x/1,2 III
    Item no.: 426947-9000-000
  • Cover glasses, high performance, D=0.17mm, box with 1000 pc.
    Cover glasses, high performance, D=0.17mm, box with 1000 pc.
    Item no.: 474030-9000-000
  • Installation Kit autocorr for Axio Observer
    Installation Kit autocorr for Axio Observer
    Item no.: 420852-9020-000
  • Immersion medium
    Immersion medium "Immersol" W 2010, oiler 20 ml
    Item no.: 444969-0000-000
  • Immersion medium Immersol G, oiler 20 ml
    Immersion medium Immersol G, oiler 20 ml
    Item no.: 462959-9901-000
  • Immersion medium Immersol Sil 406, Oiler 20 ml
    Immersion medium Immersol Sil 406, Oiler 20 ml
    Item no.: 444971-9000-000
  • Box of lens cleaning paper
    Box of lens cleaning paper
    Item no.: 462975-0000-000
Mechanical Dimensions
Transmittance curve
LCI Plan-Apochromat

These objectives have been specifically designed for Life Cell Imaging techniques and are designed for the temperature interval between 23° and 37°C. The 25x/0.8 can be used with the immersion mediums water, glycerine and oil. A single correction collar permits simple and convenient compensation for spherical aberration caused, for example, by different cover slip thicknesses, temperature drift or immersion media with a different refractive index, adjustable between water and glycerine. For the examination of living objects, water is particularly suitable as immersion medium. Furthermore, cleaning is extremely easy. For critical fluorescence examinations, cleaned glycerol is the ideal immersion medium, as it has very low autofluorescence. Therefore, glycerol and water should be preferred for the microscopy of living objects, since these are found in a medium with a similar refractive index. The "Refractive Index Mismatch" indicates the deviation of the actually available refractive index from the one that the objective has been calculated for. This deviation too can be compensated for by means of the correction collar. Used with inverted microscopes, multi-immersion objectives are ideal for the limited compensation of non-standard glass bottom thicknesses (D ≠ 0,17) or the popular use of water as immersion medium with a slightly lower numerical aperture than oil-immersion objectives.
Special emphasis has been placed on good accessibility of the optical axis with micromanipulators.

Objective LD LCI Plan-Apochromat 40x/1.2 Imm autocorr DIC M27 for water, silicon oil or glycerine immersion (CG=0.15-0.19mm) (FWD=0.40mm at CG=0.17mm) ACR functionality included. Incl. Immersol G, bottle 20 ml and Cover glasses, high performance, CG=0.17mm, box with 100 pc With serial number. Strehl ratio >90%.