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    Objective LCI

    Objective LCI "Plan-Neofluar" 25x/0.8 Imm Corr DIC M27

    Item no.: 420852-9972-000
    (no longer available)
    Description

    Objective LCI Plan-Neofluar 25x/0.8 Imm Corr DIC M27 for water, silicone oil, glycerine or oil immer...

    Full Description

    The rating of the option Definite Focus.2 is valid for use with water or glycerine immersion.

    Magnification 25x
    Numerical Aperture 0.8
    Free Working Distance [mm] 0.21 at cover glass 0.17
    Coverglass Thickness [mm] 0 - 0.17
    Thread Type M27x0.75
    Immersion Water, Silicone oil, Glycerine and Oil
    Field of View [mm] 20
    Parfocal Length [mm] 45.06
    Long Distance
    Correction Ring Yes
    Iris
    Optical System Infinity Color Corrected System (ICS)
    Flatness
    Color Correction
    Biomedical Applications
    Fluorescence Yes
    - Multichannel
    - Ultraviolet Transmission
    - Infra Red Transmission
    BrightField Yes
    Differential Interference Contrast
    High Contrast DIC
    Polarization-Optical DIC
    Phase Contrast
    VAREL Contrast
    Hoffman Modulation Contrast
    Polarization Contrast
    Materials (Reflected Light) Applications
    BrightField Yes
    BrightField/DarkField
    Reflected Light DIC
    High Contrast DIC
    DIC with circular polarized light
    Total Interference Contrast
    Polarization Contrast
    Options
    Definite Focus.2
    Confocal Microscopy Yes
    - Ultra Violet
    - VIS (visible light)
    - NLO-IR / 2 Photon
    Total Internal Reflection Fluorescence
    ApoTome Yes
    Microdissection
    • DIC slider LCI PN 25x/0.8 II
      DIC slider LCI PN 25x/0.8 II
      Item no.: 426947-0000-000
    • Adapter 30mm with optics and DIC slider mount
      Adapter 30mm with optics and DIC slider mount
      Item no.: 424516-9041-000
    • Objective heater 32/34 S1 (D)
      Objective heater 32/34 S1 (D)
      Item no.: 411860-9066-000
    • Objective ring ACR for objective sleeve cylindrical short
      Objective ring ACR for objective sleeve cylindrical short
      Item no.: 424508-0000-000
    • Transmission grid PL
      Transmission grid PL "ApoTome" for "Axioplan" 2
      Item no.: 000000-1151-003
    • Transmission grid L1
      Transmission grid L1 "ApoTome" for "Axio Imager"
      Item no.: 423664-0000-000
    • Immersion medium
      Immersion medium "Immersol" W 2010, oiler 20 ml
      Item no.: 444969-0000-000
    • Immersion medium Immersol G, oiler 20 ml
      Immersion medium Immersol G, oiler 20 ml
      Item no.: 462959-9901-000
    • Immersion medium Immersol Sil 406, Oiler 20 ml
      Immersion medium Immersol Sil 406, Oiler 20 ml
      Item no.: 444971-9000-000
    • Immersion oil
      Immersion oil "Immersol" 518 F fluorescence free, bottle 100 ml
      Item no.: 444962-0000-000
    • Immersion oil
      Immersion oil "Immersol" 518 F fluorescence free, bottle 500 ml
      Item no.: 444964-0000-000
    • Immersion oil
      Immersion oil "Immersol" 518 F fluorescence free, oiler 20 ml
      Item no.: 444960-0000-000
    • Box of lens cleaning paper
      Box of lens cleaning paper
      Item no.: 462975-0000-000
    Mechanical Dimensions
    Transmittance curve
    LCI Plan-Neofluar

    These objectives have been specifically designed for Life Cell Imaging techniques and are designed for the temperature interval between 23° and 37°C. The 25x/0.8 can be used with the immersion mediums water, glycerine and oil, while the 63x/1.3 can be used with water and glycerine. A single correction collar permits simple and convenient compensation for spherical aberration caused, for example, by different cover slip thicknesses, temperature drift or immersion media with a different refractive index, adjustable between water and glycerine. For the examination of living objects, water is particularly suitable as immersion medium. Furthermore, cleaning is extremely easy. For critical fluorescence examinations, cleaned glycerol is the ideal immersion medium, as it has very low autofluorescence. Therefore, glycerol and water should be preferred for the microscopy of living objects, since these are found in a medium with a similar refractive index. The "Refractive Index Mismatch" indicates the deviation of the actually available refractive index from the one that the objective has been calculated for. This deviation too can be compensated for by means of the correction collar. Used with inverted microscopes, multi-immersion objectives are ideal for the limited compensation of non-standard glass bottom thicknesses (D ≠ 0,17) or the popular use of water as immersion medium with a slightly lower numerical aperture than oil-immersion objectives.
    Special emphasis has been placed on good accessibility of the optical axis with micromanipulators.

    Objective LCI Plan-Neofluar 25x/0.8 Imm Corr DIC M27 for water, silicone oil, glycerine or oil immersion (CG=0-0.17mm) (FWD=0.21mm at CG=0.17mm) incl. Immersol W, bottle 20 ml