The rating of the option Definite Focus.2 is valid for use with water or glycerine immersion.
|Free Working Distance [mm]||0.21 at cover glass 0.17|
|Coverglass Thickness [mm]||0 - 0.17|
|Immersion||Water, Silicone oil, Glycerine and Oil|
|Field of View [mm]||20|
|Parfocal Length [mm]||45.06|
|Optical System||Infinity Color Corrected System (ICS)|
|- Ultraviolet Transmission|
|- Infra Red Transmission|
|Differential Interference Contrast|
|High Contrast DIC|
|Hoffman Modulation Contrast|
|Materials (Reflected Light) Applications|
|Reflected Light DIC|
|High Contrast DIC|
|DIC with circular polarized light|
|Total Interference Contrast|
|- Ultra Violet|
|- VIS (visible light)|
|- NLO-IR / 2 Photon|
|Total Internal Reflection Fluorescence|
All measures in [mm]
mech. Arbeitsabstand = mechanical working distance
Deckglas = cover glass
Objektebene = object plane
Objektfeld = object field
Ausleuchtung = illumination
Probenzugänglichkeit = specimen accessibility
Please note that due to production tolerances, the given values are typical only and not guaranteed.
These objectives have been specifically designed for Life Cell Imaging techniques and are designed for the temperature interval between 23° and 37°C. The 25x/0.8 can be used with the immersion mediums water, glycerine and oil, while the 63x/1.3 can be used with water and glycerine. A single correction collar permits simple and convenient compensation for spherical aberration caused, for example, by different cover slip thicknesses, temperature drift or immersion media with a different refractive index, adjustable between water and glycerine. For the examination of living objects, water is particularly suitable as immersion medium. Furthermore, cleaning is extremely easy. For critical fluorescence examinations, cleaned glycerol is the ideal immersion medium, as it has very low autofluorescence. Therefore, glycerol and water should be preferred for the microscopy of living objects, since these are found in a medium with a similar refractive index. The "Refractive Index Mismatch" indicates the deviation of the actually available refractive index from the one that the objective has been calculated for. This deviation too can be compensated for by means of the correction collar. Used with inverted microscopes, multi-immersion objectives are ideal for the limited compensation of non-standard glass bottom thicknesses (D ≠ 0,17) or the popular use of water as immersion medium with a slightly lower numerical aperture than oil-immersion objectives.
Special emphasis has been placed on good accessibility of the optical axis with micromanipulators.
Objective LCI Plan-Neofluar 25x/0.8 Imm Corr DIC M27 for water, silicone oil, glycerine or oil immersion (CG=0-0.17mm) (FWD=0.21mm at CG=0.17mm) incl. Immersol W, bottle 20 ml