This objective is used as an overview objective for Histology.
It permits viewing of an object field with a diameter of up to 25 mm. This means that a standard 26x76 mm slide can be entirely examined with a single move in the x-direction. Therefore an adequate field illumination is required. The only condenser which illuminate the 25 mm diameter object field (not possible anyway without a condenser) is the achromatic aplanatic universal condenser 0.9 used in the brightfield position and with swung-out front lens.
On account of its low aperture, this transmitted-light objective can be used with cover slips of any thickness or without any cover slip at all.
|Free Working Distance [mm]||2.9|
|Coverglass Thickness [mm]||0.17|
|Field of View [mm]||25|
|Parfocal Length [mm]||45.06|
|Optical System||Infinity Color Corrected System (ICS)|
|- Ultraviolet Transmission|
|- Infra Red Transmission|
|Differential Interference Contrast|
|High Contrast DIC|
|Hoffman Modulation Contrast|
|Materials (Reflected Light) Applications|
|Reflected Light DIC|
|High Contrast DIC|
|DIC with circular polarized light|
|Total Interference Contrast|
|- Ultra Violet|
|- VIS (visible light)|
|- NLO-IR / 2 Photon|
|Total Internal Reflection Fluorescence|
All measures in [mm]
mech. Arbeitsabstand = mechanical working distance
Deckglas = cover glass
Objektebene = object plane
Objektfeld = object field
Ausleuchtung = illumination
Probenzugänglichkeit = specimen accessibility
Please note that due to production tolerances, the given values are typical only and not guaranteed.
The Enhanced Contrast Plan-Neofluar universal objectives with consequentliy minimized stray light: with a visible increase in contrast important for all contrast methods: brightfield, darkfield, phase contrast, DIC, fluorescence, Pol. They feature high resolving power and brilliant, rich-contrast, completely flat images for observation and documentation. Their high transmission of wavelengths from the near UV range (>40% starting at 340nm) and the use of glass types of low autofluorescence makes these objectives ideal for epi-fluorescence microscopy. Further special-purpose members of the family are EC Epiplan-Neofluar objectives for reflected-light work, the EC Plan-Neofluar Antiflex objective for reflection contrast, and EC Plan-Neofluar Pol objectives for the requirements of polarizing microscopy.
Objective EC Plan-Neofluar 1x/0.025 M27 (FWD=3.0mm)