Objective LD A-Plan 40x/0.50 HMC​
  1. Objective LD A-Plan 40x/0.50 HMC​

Objective LD A-Plan 40x/0.50 HMC​

Objective LD A-Plan 40x/0.50 HMC

Item no.: 000000-1122-156
(no longer available)
Description

Objective LD A-Plan 40x/0.50 HMC (D=0.7-1.3) (WD=2.0mm when D=1mm)

Full Description

Special objective for Hoffman Modulation Contrast for the "Axiovert" 200. Special condenser for the objective: LD condenser 0.6 H/HMC for "Axiovert" 200 a=40mm with 4-position turret and 3 integrated slit apertures for HMC objectives A-Plan 10x, LD A-Plan 20x and 40x, polarizer and green filter, order number 000000-0430-043.

Glass thicknesses of less than 0.7mm require the following additional components for objective LD "A-Plan" 40x/0.50 HMC: Coverglass cap d=0.17-0.6, order number 422902-9901-000.

If the "Axiovert" 200 configuration does not include tubes with integrated Bertrand lens (000000-1005-828 or 000000-1005-829), the following item is required for the setting of Hoffman contrast: Auxiliary microscope d=30, order number 444830-9902-000.

The above objectives are original Zeiss objectives modified by Modulation Optics Inc., USA, in accordance with our quality guidelines. The condenser is a product from Modulation Optics Inc. which has been optimized for the "Axiovert" 200.

Hoffman Modulation Contrast is a technique for the contrasting of unlabeled specimens, developed by Dr. Hoffman, the founder of the Modulation Optics company. Its price and its contrasting quality is approximately positioned between "Varel" and DIC. Unlike DIC, however, it can also be used with Petri dishes made of plastic, since it is insensitive to birefringence. Accordingly, it is mainly used in examinations of living cells, particularly for In Vitro Fertilization (IVF).

Magnification 40x
Numerical Aperture 0.5
Free Working Distance [mm] 2.0 at cover glass 1.0
Coverglass Thickness [mm] 0.7 - 1.3
Thread Type W0.8x1/36"
Immersion Without Immersion
Field of View [mm] 23
Parfocal Length [mm] 45.34
Long Distance Yes
Correction Ring
Iris
Optical System Infinity Color Corrected System (ICS)
Color Correction achromatic
Biomedical Applications
Fluorescence Yes
- Multichannel
- Ultraviolet Transmission
- Infra Red Transmission
BrightField Yes
DIC [Differential Interference Contrast]
High Contrast DIC
PlasDIC
Phase Contrast
VAREL Contrast
Hoffman Modulation Contrast Yes
Polarization Contrast
Materials (Reflected Light) Applications
BrightField
BrightField/DarkField
Reflected Light DIC
High Contrast DIC
DIC with circular polarized light
Total Interference Contrast
Polarization Contrast
Options
Definite Focus.2
Confocal Microscopy
- Ultra Violet
- VIS (visible light)
- NLO-IR / 2 Photon
Total Internal Reflection Fluorescence
Apotome
Microdissection
  • Coverglass cap for D=0.17-0.6mm
    Coverglass cap for D=0.17-0.6mm
    Item no.: 422902-9901-000
Mechanical Dimensions
Transmittance curve
LD A-Plan

The series with large working distance represent the entry level for inverted microscopy. They are corrected for cover glass and vessel bottom thickness up to 2 mm. This means optimal in the main applications of LD objectives, such as observation through thicker cell culture vessels etc. But also the use of thinner cover glasses for example in the range of 0.17 to 0.6 mm is easily possible with the simple use of a cover glass cap. The available contrast methods are ranging from brightfield, phase contrast to VAREL, Hoffman Contrast up to PlasDIC, the Zeiss exclusive polarization optical differential interference contrast, that makes applications like ICSI now possible even in plastic vessels with high resolution and brilliant contrast. Of course they are also suitable for fluorescence applications with excitation wavelengths within the visible spectrum.

Objective LD A-Plan 40x/0.50 HMC (D=0.7-1.3) (WD=2.0mm when D=1mm)