Objective A-Plan 10x/0.25 HMC
Objective A-Plan 10x/0.25 HMC
Item no.: 000000-1122-152(no longer available)
Objective A-Plan 10x/0.25 HMC (D=0-2) (WD=3.9mm when D=1mm)
Full DescriptionSpecial objective for Hoffman Modulation Contrast for the "Axiovert" 200. Special condenser for the objective: LD condenser 0.6 H/HMC for "Axiovert" 200 a=40mm with 4-position turret and 3 integrated slit apertures for HMC objectives A-Plan 10x, LD A-Plan 20x and 40x, polarizer and green filter, order number 000000-0430-043.
If the "Axiovert" 200 configuration does not include tubes with integrated Bertrand lens (000000-1005-828 or 000000-1005-829), the following item is required for the setting of Hoffman contrast: Auxiliary microscope d=30, order number 444830-9902-000.
The above objectives are original Zeiss objectives modified by Modulation Optics Inc., USA, in accordance with our quality guidelines. The condenser is a product from Modulation Optics Inc. which has been optimized for the "Axiovert" 200.
Hoffman Modulation Contrast is a technique for the contrasting of unlabeled specimens, developed by Dr. Hoffman, the founder of the Modulation Optics company. Its price and its contrasting quality is approximately positioned between "Varel" and DIC. Unlike DIC, however, it can also be used with Petri dishes made of plastic, since it is insensitive to birefringence. Accordingly, it is mainly used in examinations of living cells, particularly for In Vitro Fertilization (IVF).
| Magnification | 10x |
| Numerical Aperture | 0.25 |
| Free Working Distance [mm] | 3.9 at cover glass 1.0 |
| Coverglass Thickness [mm] | 0 - 2 |
| Thread Type | W0.8x1/36" |
| Immersion | Without Immersion |
| Field of View [mm] | 23 |
| Parfocal Length [mm] | 45.06 |
| Long Distance | |
| Correction Ring | |
| Iris | |
| Optical System | Infinity Color Corrected System (ICS) |
| Color Correction | achromatic |
| Biomedical Applications | |
|---|---|
| Fluorescence | 
|
| - Multichannel | |
| - Ultraviolet Transmission | |
| - Infra Red Transmission | |
| BrightField |
|
| DIC [Differential Interference Contrast] | |
| High Contrast DIC | |
| PlasDIC | |
| Phase Contrast | |
| VAREL Contrast | |
| Hoffman Modulation Contrast |
|
| Polarization Contrast | |
| Materials (Reflected Light) Applications | |
| BrightField | |
| BrightField/DarkField | |
| Reflected Light DIC | |
| High Contrast DIC | |
| DIC with circular polarized light | |
| Total Interference Contrast | |
| Polarization Contrast | |
| Options | |
| Definite Focus.2 | |
| Confocal Microscopy | |
| - Ultra Violet | |
| - VIS (visible light) | |
| - NLO-IR / 2 Photon | |
| Total Internal Reflection Fluorescence | |
| Apotome | |
| Microdissection | |
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Adapter M 27x0.75 / W 0.8 H "0"
Item no.: 000000-1095-168
Note:
All measures in [mm]
mech. Arbeitsabstand = mechanical working distance
Deckglas = cover glass
Objektebene = object plane
Objektfeld = object field
Ausleuchtung = illumination
Probenzugänglichkeit = specimen accessibility
Note:
Please note that due to production tolerances, the given values are typical only and not guaranteed.
