Microscope Axioscope 7 complete system

Microscope stand Axioscope 7, TL/RL MAT MOT, 6x HD DIC encoded - lower stand part with dovetail illumination interface - transmitted-light illumination with white LED 10W, optional for HAL 12V 100W - dovetail mount for attachable stage carriers - 24 mm motorized focus lift (min. step width 78 nm), adjustable focus stop - 6-position filter wheel for dia. 36 mm filters - mount for 2-position filter slider 14x40, d=36 mm - CAN port for Colibri/HXP - integrated 24V DC 90W power unit, stabilized 100...240V AC/ 50...60Hz - country-specific power cable - ECO mode and light management control button - Snap button on right side supports on ZEISS Axiocam - Motion button on left side for motorized stage movement - USB 2.0 to PC - CAN interface for motorized stage - dust cover - filter holder in TL - upper stand part with dovetail interface for reflected illumination HAL 100 /HBO /HXP/Colibri - brightfield/darkfield reflected light illuminator LED 10W white - nosepiece 6x brightfield/darkfield DIC, M27, encoded - dovetail mount for encoded changeable reflector turrets and reflector Slider - built in illumination adapter, achromatic - built in luminous-field diaphragm slider - built in aperture stop slider - mount for filter slider A 14x40 mm d=25mm - mount for polarizer slider A 6x30 mm

Stage carrier D/A; attachable and vertically adjustable for Axioscope, to accommodate screw-on stages

Reflector turret 4x encoded, changeable, for P & C modules

Reflector module bright field ACR P&C for reflected light

Reflector module polarizer ACR P&C for reflected light

Mechanical stage 80x60 mot standard; CAN (D) for Axioscope 7 -Travel range: 80mm x 60mm - Max. velocity: 25mm/s - Smallest movement: 0.5um - Repeatability : ≤ ±3um (bi-directional, measured according to ISO 230.2) - Accuracy (full range): ≤ ±10um (measured according to ISO 230.2) - Max. payload: 1Kg

Mounting frame for one holder TCA (D) The mounting frame is accessory for technical cleanliness and applicable for: - holder TCA for filter membranes with diameter 47 mm; - holder TCA for filter membranes with diameter 50 mm; - holder TCA for the test standard.

Holder TCA for filter membrane, DM 47 (D) Holder for filter membrane, diameter 47 mm. Accessory for analysis of particulate contamination on filter membranes.

Binocular phototube 20°/23 (100:0/0:100), upright image with sliding prism, camera port with interface 60N

Camera Adapter 60N-C 2/3'' 0.63x; rotating +/- 2° for fine adjustment of camera rotation. - adjustment range: +/- 2° - maximum weight load: 2 kg

Stage micrometer for reflected light 5 + 100/100y d=0mm - 5 lines with 1mm intervals in y-direction - 100 lines with 1/100mm intervals in y-direction

Eyepiece PL 10x/23 Br. foc.

Eyepiece eyecup

Objective EC Epiplan-Neofluar 2.5x/0.06 M27 (FWD=15.1mm)

Objective EC Epiplan-Neofluar 5x/0.13 BD M27 (FWD=14.5mm)

Microscopy Workstation Compact Intel Core-i3 multilingual (O) - HP Z2 G4 Workstation - CPU: Intel Core i3-8100 3.6 GHz 4 cores 4 threads - Chipset: Intel C246 - Memory: max. 64 GB RAM - Memory Expansion Slots: 4 x DDR4-RAM modules, 2666 MHz non-ECC - PCI Express Connectors: 1 x PCI Express Generation 3 x16 1 x PCI Express Generation 3 x4 2 x PCI Express Generation 3 x1 - Grafics card onboard: Intel UHD Grafik 630 2 x Display Port 1.2 (max. 3840 x 2160 Pixel) - SSD: 1 x 256 GB M.2 NVMe - Harddisks: 1 x 6 TB SATA 7200 rpm - Optical Storage: DVD +/- RW recorder for rewriteable media - Network Controller: 1 x GbE LAN interface integrated - I/O: 6 x USB 3.0 Type A (2 x front, 4 x rear), 2 x USB 2.0 (rear) - Certifications <(>&<)> Declarations: CE, CSA, UL, FCC, CCC, KCC - Power Supply: 100 - 127 V / 200 - 240 V / 50 - 60 Hz / 500 W with country specific plus - Chassis Dimensions: (H x W x D): 435 x 169 x 356 mm - USB Mouse - Rescue Kit

System configuration 3 Windows 10 LTSC 2019 (hp Z2) (O) Installation of Windows 10 Enterprise LTSC 2019 x64 Value incl. Windows 10 license Installation of ZEN software for LSM 700, LSM 800, LSM 900 and widefieldsystems Only for Microscopy Workstation Compact hp Z2 (410201-9911-000)

Graphic Card NVIDIA Quadro P400 2GB mDP (O) Professional graphic card for Workstation Premium - 2 GB GDDR5 memory - PCI Express 3.0 Interface x16 - Hardware acceleration of DirectX 12, OpenGL 4.5 and CUDA - Support for up to three displays - Display outputs: 3 x DisplayPort 1.4 (mDP) - DisplayPort support for 3 x 32'' Display (3840 x 2160 Pixel)

Memory 16 GB (1x16) DDR4-2666 MHz non-ECC unbuffered RAM (O) consisting of 1 x 16 GB modules - only for 410201-9911-000 Workstation Compact ZEISS 65BC (hp Z2)) - can be extended to 64 GB (4 x 410201-1604-000)

Language Pack English US (O) - Keyboard with English (US) layout

Monitor TFT 24'' HP Z24n G2 (61 cm) (O) Visible diagonal: 60.96 cm, 16:10 Max. resolution: 1920 x 1200 pixel @ 60 Hz Brightness: 300 cd/m2 Contrast: 1000:1 Viewing angle: horizontal 178 degree, vertical 178 degree 3 x Video input: DisplayPort 1.2, DVI-D, HDMI 1.4 3 x USB 3.0, 2 x USB 3.1 Type-C Power consumption: Maximum 92 W, Typical 40 W, Power Saving 0.5 W Dimensions with stand (H x W x D): 50.3 cm x 53.2 cm x 21.4 cm Weight: 7.2 kg (incl. stand) Ergonomic features: Tilt: -5°to +22°, swivel: -45° to +45°, height adjustable 15 cm, Pivot rotation 90° Certification and compliance: CE, CSA, GS, TÜV, FCC, TCO Operating voltage: 100-240 V, country-specific power cable

ZEN core v3.1 Image acquisition and processing for light microscopy using Windows 10 (64bit). - User interface offers stepless scaling and zooming for optimal adjustment to the screen size -Workbench concept for customized routine jobs and workflows - Full integration of the ZEISS microscopes that can be configured in MicroToolbox 2011, Axiocam cameras and additional components. Interactive and automatic control of the individual motorized microscope components Transfer of information from encoded components into the software. - Image acquisition with b/w, rgb, high-resolution and high-sensitivity cameras, b/w images with up to 16 bits, color images with up to 3 x 16 bits. - Adjustment of the display parameters without changing the pixel values - Fully automatic assignment of geometric scaling's when acquiring an image (depending on the microscope configuration) - Recording and saving of acquisition history as meta data in CZI image format - Automatic saving of acquired images in CZI or other image formats (including meta data) - Configuration options for the graphical user interface: Generation of workbenches, creation of Job templates, execution of Job templates bars and definition of properties of standard graphic elements - Save into BMP, GIF, JPG, PNG, TIFF, WDP, WMP image formats - Navigator window - Management, visualization and printing of meta data and images - µ bar scalings - Text annotation - Image processing: standard operations for image optimization (contrast, brightness, gamma, colors, smoothing, sharpening, geometric corrections...) - Input Form Designer with automated customer data import - Data zone functionality, also burned in saved images - Interface to ImageJ software - Video recorder functionality - HDR - Acquisition of High Dynamic Range Images - Online Panorama: For acquisition of tiles images on un-coded/ un-motorized stages -Panorama function for acqusition of tiles images on manual stages - ZEN Module Manual Extended Depth of Focus (EDF) capability -Best Image function for optimal exposure and lightpath settings by choosing from selection of acquired images - ZEN Module Measurement: Interactive/dimensional measurement: length, Area, contour-based measurement data (area, box, perimeter, gray values), angle, weld seams ''(A-Measure)'', Multi-Interdistance - Loops in Job Templates to enable various number of evaluations as well as performing of Batch Mode - Contains ZEN Connect basic functionality - 2D Overlay CAD (Computer Aided Design) Files with ZEN Images, provided file formats: *.dxf, *.dwg, *.igs, *. Iges, *.rle, *.stp, *.step, *.brp - F2 hot key for 2D Image Acquisition e.g. via Footswitch - Predefining and renaming of Images and documents for Freemode - Multi-Image-view - Table processing (e.g. join multiple tables, statistical values) - Electron microscopes can be used for image acquisition - Report creation and modification of Microsoft Word report templates using ZEN Word add-in - User Management for multi-user environment incl. full integration into the Windows multi-user functionality - Data Archive (Browse Results) for managing <(>&<)> maintaining documents: searching, filtering, tagging, viewing, re-opening, structuring, exporting (e.g. Excel), importing (any file format via drag n drop) -Topography viewer for 2.5D visualization of EDF Images incl. profile line measurement Contains also the functionality of the modules: - ZEN Module Panorama - ZEN Module Manual Extended Focus - ZEN Module Measurement -ZEN Module APEER (on-site) Basic * *The APEER (on-site) Basic connector enables the execution of customized DEMO solutions by integrating modules from the APEER platform

ZEN core Technical Cleanliness Analysis Package w. Axiocam 705 pol (D) Package with Axiocam 705 pol and ZEN core Technical Cleanliness Analysis, a software module for evaluation of particulate contamination on prepared specimen. The following standards for component cleanliness, oil cleanliness and cleanliness of medical products are supported: VDA 19.1, ISO 16232, ISO 4406, ISO 4407, NAS 1638, SAE AS 4059, VDI 2083 (Sheet 1). The scope of delivery includes the digital camera Axiocam 705 pol and as part of the ZEN core Technical Cleanliness Analysis software preconfigured automated workflows for technical cleanliness analysis and rating of single or multiple specimen for the user roles Supervisor and Operator. Provided job templates (I-II): (I) automated workflow for acquisition of multichannel images with different polarization directions in combination with Axiocam 705 pol mainly for the evaluation of component cleanliness and the rating of technical cleanliness of medical products. The use of latest camera sensor technology with on-chip polarization enables a workflow with one single specimen acquisition instead of double image acquisition of the complete specimen area with different contrast methods. This leads to a significant time saving. (II) automated workflow for acquisition of single channel images mainly for oil analysis. All job templates cover image acquisition, image analysis and image processing, result presentation in interactive views for fast inspection and revision of detected particles, reports with results of characteristic values and standard specific methods per tested specimen. Automated storage of generated results and inspection views in the data archive. Inspection and export of archived data. Camera specification: High Performance microscopy camera incl. driver software 64 bit USB 3.0 PCIe x1 interface, USB 3.0 cable 3 m and protection glass (wideband coated) Sensor Type: SONY IMX250 MZ Exmor CMOS, SONY Pregius Global Shutter Monochrome with 2x2 pixel specific polarization filter mask (90°, 45°, 135°, 0°) Number of Pixels: 2464 (H) x 2056 (V) = 5.07 Mega pixel Pixel Size: 3.45 µm x 3.45 µm Chip Size: 8.5 mm x 7.1 mm, equivalent to 2/3'' (diagonal 11.1 mm) Spectral Range: With protection glass approx. 350 nm to 1000 nm Full Well Capacity: Approx. 11,000 e at gain 1x (typ.) Adjustable Signal Amplification: 1x, 2x, 4x, 8x, 16x Selectable Resolution: H x V Binning Factor (1x1 to 5x5 digital) 2464 x 2056 1x1 Basic Resolution 1232 x 1028 2x2 816 x 684 3x3 608 x 514 4x4 480 x 410 5x5 (at 2x2 combined analog and digital binning for further increase of sensitivity) Effective Pixel Count per Polarization Channel: H x V Mega pixel 1232 x 1028 1.26 H x V Subsampling Factor 2464 x 2056 1x1 1232 x 1028 2x2 Live Frame Rates: (1 ms exposure, default setting) H x V Frame Rate (fps) 2464 x 2056 30 Frame Rates for Time Series Recording: (1 ms exposure, further depending on hardware and software configuration) H x V (ROI) Frame Rate (fps) 2464 x 2056 60 1920 x 1080 115 1232 x 1028 173 (2x2 subsampling, full field of view) 1024 x 1024 121 512 x 512 235 1920 x 256 436 Read Noise Full Well Capacity Dynamic Range (at gain) 2.20 e (1x) 11,000 e 1:5000 1.74 e (2x) 5,000 e 1:3100 1.48 e (4x) 2,700 e 1:1800 1.29 e (8x) 1,300 e 1:1300 1.15 e (16x) 690 e 1: 600 HDR Mode Dynamic Range 1:25,000 Software Supported Camera Operation: Exposure time adjustment with continuous, automatic or manual mode Adjustable over or under-exposure mode Optional measurement on defined target area (spot-meter) Adjustable color saturation for pseudo color representation of polarization strength Readout of Sensor Sub Regions (''ROI''): Adjustable Digitization: 8, 12, 14 bit / pixel (8 out of 14 bit or compression mode) Dark Current: <(><<)> 0.5 e/p/s @ 18 °C sensor temperature Cooling: Stabilized temperature to 18 °C (for ambient temperatures from 18 °C to 30 °C) Exposure Time Range: 0.1 ms to 60 s Hardware Trigger: Trigger-In for exposure time control Trigger-Out for exposure time Readout time and trigger ready status (further accessories required) Status-LED for Camera: Color coded operation status, dimmable Camera Interface: USB 3.0 (Optional operation with USB 2.0 at reduced frame rate) Optical Interface: C-Mount Thread Depth for Objectives: Max. 5 mm Si

ZEN Module Tiles & Positions Module for automatic scanning of predefined sample areas, plus acquisition of images according to a position list. Supports focus correction maps, stitching and shading correction. Compatible with software autofocus.

ZEN Module Autofocus Module for determination of the optimal focus position in the sample. The autofocus works with transmitted, reflected and fluorescence light. Definition of an autofocus region in the camera image.