Inverted microscope Axiovert 5 TL FL SCB f/ Ph1/2 DIC FL

Microscope stand Axiovert 5 TL FL SCB with integrated Smart Control Box - 6-position nosepiece B DIC M27, encoded with 10x29 mm slider mount - 6-position reflector turret, encoded for P&C modules - Carrier transmitted-light with integrated white LED 10W (average LED lifetime > 60000 hrs) and 2-position filter slider, d=32 mm with phosphorescence block filter - Reflected light illumination with interface for Colibri / HBO / HXP / X-Cite Xylis® / HAL 100, with slider mounts for luminous-field diaphragm, aperture stop and filter slider - manual coarse/fine focus drive - 13 mm focus lift with adjustable focus stop - TL/FL switching - snap button for ZEISS Axiocam - ECO mode - Light manager for TL LED and Colibri 3 - CAN port for Colibri 3 - Smart Control Box - for stand-alone operation (without PC) of microscope stand functions with ZEISS Axiocam (105 R2, 202, 208, 305, 705) via On Screen Display and for usage with Labscope (Windows and iOS) - Automatic features for camera control, Image enhancement functions and readout of encoded microscope functions - Provided interfaces: HDMI, USB 3.0 Type-C and Type-A, USB 2.0 Type- A, Ethernet, Micro-D - Wi-Fi compatibility via USB Wi-Fi adapter and router - USB 3.0 flash drive, Type-C and Type-A - USB 3.0 cable 0.5 m for Axiocam 305 - USB 2.0,Type B interface to PC - external power adaptor input: 100-240V AC, 50/60Hz output: 24V DC, 5A - country specific power cable - Dust protection set

Intermediate phototube H=50mm, left port - Camera port with interface 60N - Slider mount with 2 clickstop positions Additionally required: - Slider with mirror 100% - Slider with beam splitter 50% - Slider with beam splitter 20/80%

Slider with Beam Splitter 20/80% for Intermediate Phototube - 2 click stop positions for 100vis/20vis:80doc

Light shield to block ambient light; Axiovert for Axiovert 5/7 and Axio Vert.A1

Antiglare screen for Axio Observer/Axiovert

llumination adapter A, achromatic - for Axiovert 5/7 and Axio Vert.A1 - for achromatization of the reflected light beam path - especially recommended for fluorescence microscopy with shorter excitation wavelengths

Solid-State Light source Colibri 3, Type RGB-UV 4-channel fluorescence light source with integrated control unit in Axioscope and Axiovert 5 TL FL SCB for continuous brightness adjustment, quickly switchable and adjustable by stand or - by Stand alone operation mode via, OnScreenDisplay (OSD) for Axioscope with in Axiocam 202/208 - by Stand alone mode via OnScreenDisplay (OSD) for Axiovert 5 TL FL SCB with Axiocam 105 R2/202/208/305/705 - software Labscope or ZEN Equipped with 4 solid state LED lamps. - Red (625nm) for excitation of Cy5, Alexa 631, TOTO-3 and similar dyes - Green (565nm) for excitation of Cy3, TRITC, DsRed and similar dyes - Blue (470nm) for excitation of eGFP, Fluo4, FITC and similar dyes - UV (385nm) for excitation of DAPI, Alexa 405, Hoechst 33258

DIC slider LD PN 20x/0.40 II

DIC slider LD PN 40x/0.60 II

DIC slider LD PN 63x/0.75 II

DIC slider EC PN 10x/0.30 I, 20x/0.50 II, PA 20x/0.80 II

Analyzer module DIC ACR P&C shift free for transmitted light optimized for DIC in transmitted light, not suitable for polarization. Pol filter for imaging free from pixel shift, almost without wedge error.

Mechanical stage 130x85 R/L for Axiovert - for Axiovert 5 and Axio Vert.A1 - manual 3-plate mechanical stage - for mounting frames K 160 x 110 mm - max. 3 kg sample load

Universal mounting frame K for: - Petri dishes dia. 24...68 mm - slides (max. length 120 mm) frame size 160 x 110 mm

Binocular tube 45°/23 - upright and unreversed image

Green filter, d=32x2 mm for contrast enhancement for phase contrast and b/w image recording

Neutral-density filter 0.05 for condenser - for the use at transmitted-light brightfield with alternating Ph, PlasDIC, iHMC - to be inserted into free position of LD condensers with modulator disk

Filter Set 90 HE LED with Reflector Module FL EC P<(>&<)>C (E) Filter Set 90 HE LED shift free for use with ZEISS LED fluorescence light sources with excitation wavelengths 385, 475, 555 and 630 nm. Consisting of filters QBS 405 + 493 + 575 + 653 and QBP 425/30 + 514/30 + 592/25 + 709/100 inserted in reflector module, reflection avoided through tilted mount for emission filters.

Camera Adapter 60N-C 2/3" 0.63x

Eyepiece PL 10x/23 Br. foc.

Eyepiece eyecup

Objective EC Plan-Neofluar 10x/0.3 Ph1 M27 (FWD=5.2mm)

Objective EC Plan-Neofluar 5x/0.16 Ph1 M27 (FWD=18.5mm)

Objective LD Plan-Neofluar 63x/0.75 Corr Ph2 M27 (CG=0-1.5mm) (FWD=2.2mm at CG=0mm and FWD=1.2mm at CG=1.5mm), incl. Cover glasses, high performance, CG=0.17mm, box with 100 pc.

Objective LD Plan-Neofluar 40x/0.6 Corr Ph2 M27 (CG=0-1.5mm) (FWD=3.3mm at CG=0mm and FWD=2.5mm at CG=1.5mm)

Objective LD Plan-Neofluar 20x/0.4 Corr Ph2 M27 (CG=0-1.5mm) (FWD=8.4mm at CG=0mm and FWD=7.4mm at CG=1.5mm)

LD condenser 0.55 for H Ph PlasDIC DIC - 5-position modulator disk, freely configurable - centerable positions, position 1 with aperture stop - for objectives 5x - 100x - variable working distance from 30 to 38 mm

Phase stop Ph 2/0.55 for condenser

Phase stop Ph 1/0.55 for condenser

Condenser module DIC I/0.55 with polarizer

Condenser module DIC II/0.55 with polarizer

Microscopy Camera Axiocam 305 mono R2 (D) Microscopy camera incl. driver software 64bit, USB 3.0 PCIe x1 interface, USB 3.0 cable 3 m and BK7 protection glass (coated) Number of Pixels: 2464 (H) x 2056 (V) = 5.07 Mega pixel color Sensor type: SONY IMX264 Exmor Pregius Global Shutter CMOS Pixel size: 3.45 µm x 3.45 µm Chip size: 8.5 mm x 7.1 mm, equivalent to diagonal 11.1 mm (2/3" form factor) Spectral range: app. 350 nm to 1000 nm Full Well Capacity: Approx. 10,500 e Selectable Resolution: H x V Binning Factor 2464 x 2056 1x1 Basic Resolution 1232 x 1028 2x2 816 x 684 3x3 608 x 514 4x4 480 x 410 5x5 H xV Subsampling Factor 2464 x 2056 1x1 Basic Resolution 1232 x 1028 2x2 Live frame rates (depending on hardware and software configuration): H x V Frame Rate@1ms 2464 x 2056 36 fps 1232 x 1028 88 fps (2x2 Subsampling) Frame rates for time series recording (depending on computer hardware and software configuration) H x V (ROI) Frame Rate 2464 x 2056 36 fps 2048 x 2048 36 fps 1920 x 1080 67 fps 1024 x 1024 70 fps 512 x 512 136 fps 256 x 256 255 fps 128 x 128 456 fps Readout of Sensor Sub-Regions ("ROI"): Adjustable Digitization: 8and 12 Bit / Pixel Dynamic Range: Typical > 4800 : 1 (>73 dB) at typ. 2.2 e readout noise Dark current: < 1.0 e/p/s @ 25°C Integration Time: 0.1 ms to to 4 s Cooling: Temperaturestable @ 25°C for ambient temperatures between 18°C and 30°C Status-LED for Camera: color coded operation status, dimmable Interface (camera): USB 3.0 interface Optional operation with USB 2.0 connection at reduced frame rate Optical Interface: C-Mount Thread depth for objectives: max. 5 mm Size / Weight: approx. App. 10.8 cm x 7.8 cm x 4.3 cm (2.3"x3.2"x1.7") / 580 g Housing: blue anodized aluminum, with cooling fins on top, 1/4" photo thread for tripod mount Registration: CE Power Supply: Max. 4W, power supply provided by USB 3.0 bus for camera electronics Environmental conditions: 5° C +35° Celsius, max 80% relative air humidity, not condensing, free air circulation required Supported Operating Systems: for ZEN: Windows 10 x64 Supported Application Software: ZEN 3.6 (blue edition, Hotfix 3.6.6) or higher, ZEN core 3.5 (Hotfix 3.5.4) or higher The required application software is available via the ZEISS Download Center.