Objective Assistant

Objective AssistantList of Objectives

Objective data

Brochure: Objectives from Carl Zeiss (5 MB)
Objective Class: Fluar
Widest apertures, high transmission already from 340nm

Transmittance curve
Objective Fluar 5x/0.25
Price Price
Magnification 5x
Numerical Aperture 0,25
Free Working Distance [mm] 12,5
Coverglass Thickness [mm] 0,17
Thread Type W0,8x1/36"
Immersion Without Immersion
Field of View [mm] 23
Parfocal Length [mm] 45,06
Long Distance (LD)
Correction Ring (Corr)
Iris (Iris)
Optical System Infinity Color Corrected System (ICS)
Color Correction
Biomedical Applications
- Multichannel
- Ultraviolet Transmission
- Infra Red Transmission
BrightField (B)
Differential Interference Contrast (DIC)
High Contrast DIC (HC DIC)
PlasDIC Contrast
Phase Contrast (PH)
VAREL Contrast
Hoffman Modulation Contrast (HMC)
Polarization Contrast (POL)
Materials (Reflected Light) Applications
BrightField (B)
BrightField/DarkField (BD)
Reflected Light DIC (RL DIC)
High Contrast DIC (HC DIC)
DIC with circular polarized light (C-DIC)
Total Interference Contrast (TIC)
Polarization Contrast (POL)
Definite Focus.2
Confocal Microscopy
- Ultra Violet
- VIS (visible light)
NLO-IR / 2 Photon
Total Internal Reflection Fluorescence (TIRF)
Objective Fluar 5x/0.25

Objective Fluar 5x/0.25 (WD=12.5mm)
Mechanical Dimensions
Click to enlarge

All measures in [mm]

mech. Arbeitsabstand = mechanical working distance
Deckglas = cover glass
Objektebene = object plane
Objektfeld = object field
Ausleuchtung = illumination
Probenzugänglichkeit = specimen accessibility

Transmittance curve
Click to enlarge

Please note that due to production tolerances, the given values are typical only and not guaranteed.

The recommended adapter M27x0.75 / W0.8 H"0" does only fit with objectives, that have a [1] printed behind the order number 440125.

 Fitting Accessories for
 440125-0000-000 Objective Fluar 5x/0.25
 Other Accessories
Adapter M 27x0.75 / W 0.8 H "0", Item Number: 000000-1095-168
Adapter M 27x0.75 / W 0.8 H "0"
Item Number: 000000-1095-168
Adapter M 27x0.75 / W 0.8 H "0"


The contents of the basket can be:
+ ordered online
+ printed as a fax order
+ requested as a written quotation
+ saved as a shopping list