 
            Objective EC Plan-Neofluar 100x/1.30 Oil M27
Objective EC Plan-Neofluar 100x/1.30 Oil M27
Item no.: 420490-9900-000Objective EC Plan-Neofluar 100x/1.30 Oil M27 (FWD=0.20mm), incl. Immersol 518 F, oiler 20ml and Cover glasses, high performance, CG=0.17mm, box with 100 pc.
Full DescriptionNot recommended for operating of Apotome with UV excitation (DAPI).
| Magnification | 100x | 
| Numerical Aperture | 1.3 | 
| Free Working Distance [mm] | 0.20 | 
| Coverglass Thickness [mm] | 0.17 | 
| Thread Type | M27x0.75 | 
| Immersion | Oil | 
| Field of View [mm] | 25 | 
| Parfocal Length [mm] | 45.06 | 
| Long Distance | |
| Correction Ring | |
| Iris | |
| Optical System | Infinity Color Corrected System (ICS) | 
| Color Correction | semi-apochromatic | 
| Biomedical Applications | |
|---|---|
| Fluorescence |   | 
| - Multichannel | |
| - Ultraviolet Transmission | |
| - Infra Red Transmission | |
| BrightField |   | 
| DIC [Differential Interference Contrast] | |
| High Contrast DIC |   | 
| PlasDIC | |
| Phase Contrast | |
| VAREL Contrast | |
| Hoffman Modulation Contrast | |
| Polarization Contrast | |
| Materials (Reflected Light) Applications | |
| BrightField | |
| BrightField/DarkField | |
| Reflected Light DIC | |
| High Contrast DIC |   | 
| DIC with circular polarized light | |
| Total Interference Contrast | |
| Polarization Contrast | |
| Options | |
| Definite Focus.2 | |
| Confocal Microscopy |   | 
| - Ultra Violet | |
| - VIS (visible light) | |
| - NLO-IR / 2 Photon | |
| Total Internal Reflection Fluorescence | |
| Apotome |   | 
| Microdissection |   | 
- 
                                                          Objective heater 22.5/34 S1 (D)Item no.: 411860-9064-000Objective heater 22.5/34 S1 (D)Item no.: 411860-9064-000  Objective heater 22.5/34 S1 (D) - with oil discharge channel - clamping via front dia. 22.5 mm - max. objective dia. 34 mm - temperature range of room temperature up to +45°C 
- 
                                                          Temperable Objective Ring 22.5/32.5 mm S1 (D)Item no.: 411882-9081-000Temperable Objective Ring 22.5/32.5 mm S1 (D)Item no.: 411882-9081-000  Temperable Objective Ring 22.5/32.5 mm S1 (D) clamping via front dia. 22.5 mm, max. objective dia. 32.5 mm 
- 
                                                          Objective ring ACR for objective sleeve cylindrical longItem no.: 424511-0000-000Objective ring ACR for objective sleeve cylindrical longItem no.: 424511-0000-000  Objective ring ACR for objective sleeve cylindrical long 
- 
                                                          Adapter 30mm with optics and DIC slider mountItem no.: 424516-9041-000Adapter 30mm with optics and DIC slider mountItem no.: 424516-9041-000  Adapter 30mm with optics and DIC slider mount for adaptation of objectives M27 with parfocal length of 45mm 
- 
                                                          DIC slider EC PN 40x/0.75 II, EC PN 100x/1.30 IIIItem no.: 426945-0000-000DIC slider EC PN 40x/0.75 II, EC PN 100x/1.30 IIIItem no.: 426945-0000-000  DIC slider EC PN 40x/0.75 II, EC PN 100x/1.30 III 
- 
                                                          Immersion suction ring d=22.5 (10 pcs.)Item no.: 444822-0000-000Immersion suction ring d=22.5 (10 pcs.)Item no.: 444822-0000-000  Immersion suction ring d=22.5 (10 pcs.) 
- 
                                                          Immersion oil Immersol 518 F fluorescence free, bottle 100 mlItem no.: 444962-0000-000Immersion oil Immersol 518 F fluorescence free, bottle 100 mlItem no.: 444962-0000-000  Immersion oil Immersol 518 F fluorescence free, bottle 100 ml (ISO 8036-1/2, ne=1.518 (23°C), halogen-free) 
- 
                                                          Immersion oil Immersol 518 F fluorescence free, bottle 500 mlItem no.: 444964-0000-000Immersion oil Immersol 518 F fluorescence free, bottle 500 mlItem no.: 444964-0000-000  Immersion oil Immersol 518 F fluorescence free, bottle 500 ml (ISO 8036-1/2, ne=1.518 (23°C), halogen-free) 
- 
                                                          Box of lens cleaning paper (300 sheets)Item no.: 462975-0000-000Box of lens cleaning paper (300 sheets)Item no.: 462975-0000-000  Box of lens cleaning paper (300 sheets) 
- 
                                                          Cover glasses, high performance, D=0.17mm, box with 1000 pc.Item no.: 474030-9000-000Cover glasses, high performance, D=0.17mm, box with 1000 pc.Item no.: 474030-9000-000  Cover glasses, high performance, D=0.17mm, box with 1000 pc. Size 18x18 mm² type 1 1/2 H as per ISO 8255-1 with restricted thickness-related tolerance D=0.17 mm +/- 0.005 mm refractive index = 1.5255 +/- 0.0015, Abbe number = 56 +/-2 recommended for applications with high numerical aperture objectives 
Note:
All measures in [mm]
mech. Arbeitsabstand = mechanical working distance
Deckglas = cover glass
Objektebene = object plane
Objektfeld = object field
Ausleuchtung = illumination
Probenzugänglichkeit = specimen accessibility
 
                                            Note:
Please note that due to production tolerances, the given values are typical only and not guaranteed.
 
                                            The Enhanced Contrast Plan-Neofluar universal objectives with consequentliy minimized stray light: with a visible increase in contrast important for all contrast methods: brightfield, darkfield, phase contrast, DIC, fluorescence, Pol. They feature high resolving power and brilliant, rich-contrast, completely flat images for observation and documentation. Their high transmission of wavelengths from the near UV range (>40% starting at 340nm) and the use of glass types of low autofluorescence makes these objectives ideal for epi-fluorescence microscopy. Further special-purpose members of the family are EC Epiplan-Neofluar objectives for reflected-light work, the EC Plan-Neofluar Antiflex objective for reflection contrast, and EC Plan-Neofluar Pol objectives for the requirements of polarizing microscopy.
Objective EC Plan-Neofluar 100x/1.30 Oil M27 (FWD=0.20mm), incl. Immersol 518 F, oiler 20ml and Cover glasses, high performance, CG=0.17mm, box with 100 pc.