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Objective "Fluar" 5x/0.25 M27

Objective Fluar 5x/0.25 M27

Item no.: 420130-9900-000

Objective Fluar 5x/0.25 M27 (FWD=12.5mm)

Full Description
Magnification 5x
Numerical Aperture 0.25
Free Working Distance [mm] 12.5
Coverglass Thickness [mm] 0.17
Thread Type M27x0.75
Immersion Without Immersion
Field of View [mm] 23
Parfocal Length [mm] 45.06
Long Distance
Correction Ring
Optical System Infinity Color Corrected System (ICS)
Color Correction
Biomedical Applications
Fluorescence Yes
- Multichannel
- Ultraviolet Transmission
- Infra Red Transmission
BrightField Yes
DIC [Differential Interference Contrast]
High Contrast DIC
Phase Contrast
VAREL Contrast
Hoffman Modulation Contrast
Polarization Contrast
Materials (Reflected Light) Applications
Reflected Light DIC
High Contrast DIC
DIC with circular polarized light
Total Interference Contrast
Polarization Contrast
Definite Focus.2
Confocal Microscopy Yes
- Ultra Violet
- VIS (visible light)
- NLO-IR / 2 Photon
Total Internal Reflection Fluorescence
Microdissection Yes
  • Objective ring ACR for objective sleeve cylindrical short
    Objective ring ACR for objective sleeve cylindrical short
    Item no.: 424508-0000-000
  • Adapter 30mm with optics and DIC slider mount
    Adapter 30mm with optics and DIC slider mount
    Item no.: 424516-9041-000
Mechanical Dimensions
Transmittance curve

These objectives have been designed especially for qualitative and quantitative analysis of ion movements and for particularly critical fluorescence methods (e.g. chromosome studies in human and cytogenetics). These objectives are distinguished by high numerical apertures and highest transmission at wavelengths even of 340 nm. Field flattening is sufficient up to a field of 20 mm.

Objective Fluar 5x/0.25 M27 (FWD=12.5mm)