Axio Imager section Advanced

Axio Imager.Z2 microscope stand with Z-drive mot. and TFT monitor - heavy duty focusing drive motorized, stepsize 10 nm, with fine drive knob and fine drive disk, flat with scale, changeable - Light Control mot. - main controller incl. USB-A, USB-C and TCP/IP interface - transmitted-light illumination mot. - external power unit 12V DC 100W, stabilized, 100V...240V AC/50...60Hz/230VA, - USB cable and country-specific line cable

Fine drive disk, flat with scala for Axio Imager 2, changeable

Path deflecting mirror 100% for camera deflection, 34x46x4 mm

Camera path deflection on the left side, mot., interface 60N motorized slider with 2 switching positions, pos. 1 = 100% vis, pos. 2 for the optional mounting of path-deflecting mirror 100% = 100% doc or beam splitter 50% = 50% vis : 50% doc

6-position objective nosepiece, HD DIC M27 mot.

Stage carrier, detachable with condenser carrier for Axio Imager suitable for sample heights from 0 mm up to 25 mm

10-position reflector turret mot. ACR, for P&C modules with automatic filter set and contrast module recognition

Docking station for TFT-Display upgrading module for the TFT-Display of motorized stands Axio Imager and Axio Observer - free positioning on desktop - Control of z-drive and circular operation key unit - mechanical interface to attach Electronic Coaxial Drive - TFT-Display 30°-45° tiltable

Illuminator VIS-LED for mounting on the transmitted-light, reflected-light illuminator, included LED lamp module for VIS-LED - color temperature 5600 K - average lifetime > 10,000 hours - control signals: Trigger-input (BNC) for controlling, max. trigger frequency ca. 100 Hz - usable for objectives from 2.5x - incl. neutral-density filter 0.12 D=25x2 for use with digital microscopy cameras

Reflected-light illuminator for FL and HD for Axio Imager with shutter and with openings for manual stop sliders and filter wheels. Transmission range 340-1100 nm. With 340 nm and 1100 nm ~40%.

Shutter for transmitted-light illumination switching time < 200 ms switching frequency max. 2 Hz

Reflector module FL EC P&C Reflection avoided through tilted mount for emission filters, max. filter thickness: 5 mm

Solid-State Light Source Colibri 7, Type FR-R[G/Y]BV-UV 7-channel fluorescence light source with integrated control unit for continuous brightness adjustment, quickly switchable and stable on the long term. With integrated monitor diode for repetitious brightness adjustment. Equipped with 7 excitation lines. Far Red (735nm) for excitation of Cy7 and similar dyes Red (630nm) for excitation of Cy5, Alexa 631, TOTO-3 and similar dyes Yellow (590nm) for excitation of mCherry, Alexa 568, mPlum and similar dyes Green (555nm) for excitation of Cy3, TRITC, DsRed and similar dyes Blue (475nm) for excitation of eGFP, Fluo4, FITC and similar dyes Violet (430nm) for excitation of eCFP, Lucifer Yellow, Alexa 430 and similar dyes UV (385nm) for excitation of DAPI, Alexa 405, Hoechst 33258 and similar dyes A country-specific power cable and a desktop power supply are included in delivery.

Scanning Stage 130x85 STEP (D) for Axio Imager - stepper motor with 2 mm spindle pitch - connection to stepper controller - travel range 130 mm x 85 mm - max speed 50 mm/s - resolution: 0.1 µm - reproducibility: +/- 1 µm - absolute accuracy: +/- 5 µm - travel range adjustable - fixed motor cable with exit on stage back side; left - for mounting frames 160 x 116 mm and Z-PIEZO

Universal mounting frame A-K (D) - frame size 160x116mm

Binocular tube 30°/23, reversed image, Axio Imager

Filter set 70 HE Alexa 430 shift free (E) EX BP 430/60, BS FT 500, EM BP 550/100

Filter Set 115 Cy7 Consisting of: EX BP 710/87 BS 760 EM BP 814/91

Filter Set 96 HE BFP shift free (E) EX BP 390/40, BS FT 420, EM BP 450/40

Filter set 64 HE mPlum shift free (E) EX BP 587/25, BS FT 605, EM BP 647/70

Filter set 43 HE Cy 3 shift free (E) EX BP 550/25, BS FT 570, EM BP 605/70

Filter set 38 HE eGFP shift free (E) EX BP 470/40, BS FT 495, EM BP 525/50

Filter set 50 Cy 5 shift free (E) EX BP 640/30, BS FT 660, EM BP 690/50

Camera Adapter 60N-C 1" 1.0x

Eyepiece PL 10x/23 Br. foc.

Eyepiece eyecup

Objective EC Plan-Neofluar 5x/0.16 M27 (FWD=18.5mm)

Objective Plan-Apochromat 10x/0.45 M27 (FWD=2.1mm)

Objective Plan-Apochromat 20x/0.8 M27 (FWD=0.55mm), incl. Cover glasses, high performance, CG=0.17mm, box with 100 pc Lead-free

Objective Plan-Apochromat 63x/1.4 Oil DIC M27 (FWD=0.19mm), incl. Immersol 518 F, oiler 20ml and Cover glasses, high performance, CG=0.17mm, box with 100 pc.

Universal condenser achromatic-aplanatic 0.9 H D Ph DIC, mot. with motorized front lens, aperture diaphragm and turret disk, with centerable Ph1, Ph2, Ph3 and D stops, already integrated, 3 mounts for DIC condenser modules, 1 separate position for bright field. For objective magnifications 1.0x-100x, FWD=1.2mm

Stage Controller XY STEP SMC 2009 Control unit for Maerzhaeuser XY-Stages with stepper motors. - bidirectional communication to microscopes with CAN - position display on microscopes with TFT display and option for objective depended travel speed - 2 CAN interfaces - alternative connection through USB or RS232 - power supply 100...240V AC/50...60Hz - incl. USB-cable, CAN-cable and plug-in power unit with country specific power cord

Joystick XY; CAN (D) for mechanical stages mot; CAN, scanning stages; CAN and stage controller XY STEP SMC 2009 - 2 speed ranges selectable - direct cable connection to CAN stage or to stage controller XY STEP SMC 2009

Extension cable for stage control 1.6 m (D) for usage with joystick, trackball or coaxial drive. Recommended for adaptation of cable length when a docking station is used.

Slider assembly Apotome 3 for Axio Imager consisting of: slider with control electronics and transmission grids with 3 frequencies, reflector module calibration Apotome ACR P&C for LED and white light source, mirror and fluorescence test slides, cable CAN-BUS, case.

Microscopy Workstation Premium (HP Z8 G5) (O) HP Z8 Fury G5 Workstation - Chipset: Intel W790 - Memory Expansion Slots: 16 x DDR5, RDIMM, ECC, 4800 MHz Modules - Memory: max. 1024 GB RAM - PCI Express Connectors: 2 x PCI Express Generation5 x16 1 x PCI Express Generation 5 x4 2 x PCI Express Generation 4 x16 2 x PCI Express Generation 4 x4 1 x PCI Express Generation 3 x8 - SSD: 1 x 2 TB M.2 NVMe - Hard Drives: 2 x 12 TB SATA 7200 rpm (configured as 10 TB RAID 1hard drive) - Network Controller: 2 x GbE LAN interface (RJ 45) - USB: 2 x USB 3.2 Gen2x2 type C (front), 8 x USB 3.1 Gen 1 type A (2 x front, 6 x rear) - Certifications <(>&<)> Declarations: CE, CSA, UL, CCC, KCC - Power Supply: 100 - 240 V / 50 - 60 Hz / 1450 W with country specific plug - Chassis Dimensions: (H x W x D): 445 x 216 x 555 mm - USB Mouse, Recovery kit

System Configuration 2 for HP Z8 G5 (O) Installation of Windows 10 IoT Enterprise LTSC 2021 x64 incl. Windows 10 License - with ZEN software installation and system integration of respective system - for Celldiscoverer 7, LSM 900, LSM 980, Elyra 7, Lattice SIM, Axioscan 7, Lattice Lightsheet - only for 410203-9914-000 Microscopy Workstation Premium HP Z8 Fury G5

Processor Intel Xeon W7-3455 (HP Z8 G5) (O) High performance processor configuration - Intel Xeon W7-3455 (2.5 GHz, 67.5 MB cache, 24 cores) - only for 410203-9914-000 Microscopy Workstation Premium HP Z8 Fury G5

Memory 32 GB (1x32) DDR5 4800 MHz ECC reg RAM (HP Z8 G5) (O) consisting of 1 x 32 GB module - DDR5 4800 MHz ECC registered DIMM - can be extended to 64 GB (2 x 410303-3206-000), 128 GB (4 x 410303- 3206-000) or 256 GB (8 x 410303-3206-000) - only for 410203-9914-000 Microscopy Workstation Premium HP Z8 Fury G5

Graphics Card NVIDIA RTX A4000 16 GB DP (O) Professional graphics card for Microscopy Workstation - 16 GB GDDR6 memory - PCI Express 4.0 Interface x16 - Hardware acceleration of DirectX 12.07, OpenGL 4.68 and CUDA - Support for up to four displays - Display outputs: 4 x DisplayPort 1.4a (DP) - DisplayPort support for 4 x 32" Display (3840 x 2160 Pixel)

Network Adapter 2 x 10 GbE RJ45 (HP Z8 G5) (O) - Additional network adapter 2 x 10 GbE RJ45 (HP Z8 G5) e.g. for connection of storage systems - only for 410203-9914-000 Microscopy Workstation Premium HP Z8 Fury G5

Language Pack English US (O) - Keyboard with English (US) layout

Monitor TFT 27" HP Z27q G3 (68 cm) (O) Visible diagonal: 68 cm, 16:9 Maximum resolution: 2560 x 1440 pixel @ 60 Hz Brightness: 350 nits Contrast: 1000:1 Viewing angle: horizontal 178 degree, vertical 178 degree Video input: DisplayPort 1.4, HDMI 2.0 Data connectors: 1 x USB Type B (upstream), 4 x SuperSpeed USB Type-A 5Gbps Power consumption: Maximum 69 W, Typical 35 W, Standby 0.5 W Dimensions incl. stand (H x W x D): 53.0 cm x 61.2 cm x 20.5 cm Weight incl. stand: 5.8 kg Ergonomic features: Tilt: -5°to +20°, Swivel: -45° to +45°, Height adjustable 15 cm, Pivot rotation: -90° to +90° Certification and compliance: CE, CB, GS, TÜV, FCC, TCO Operating voltage: 100-240 V, country-specific power cable

30m Patch Cable CAT7

Microscopy Camera Axiocam 820 mono High performance microscopy monochrome camera incl. driver software 64bit, Interface card USB 3.1 Gen2 PCIe x4, dual USB 3.0 cable 3 m Sensor Type: SONY IMX541 CMOS, monochrome, Global Shutter "Backside Illuminated" Number of Pixels: 4512 (H) x 4512 (V) = 20.3 Mega Pixels Pixel Size: 2.74 µm x 2.74 µm Chip Size: 12.4 mm x 12.4 mm, (square field of view), equivalent to 1.1" (17,4 mm diagonal) Spectral Range: With protection glass. 350 nm to 1000 nm Peak QE: 86% @ 520 nm (typ.), "backside illuminated" design Full Well Capacity: Approx. 10,000 e at gain 1x (typ.) Adjustable Signal Amplification: 1x, 2x, 4x, 8x, 16x Selectable Resolution: H x V Binning Factor (digital) 4512 x 4512 1x1 basic pixelcount 2256 x 2256 2x2 1504 x 1504 3x3 1128 x 1128 4x4 902 x 902 5x5 (at 2x2 combined analog and digital binning for further increase of sensitivity) H x V Subsampling Factor(full field of view) 4512 x 4512 1x1 2256 x 2256 2x2 Live frame rates (depending on hardware and software configuration): Exp. Time 1ms H x V Frame Rate 4512 x 4512 >20 fps (High Res mode, high CPU load) 2256 x 2256 >30 fps (default: Fast Mode, reduced CPU load) Frame rates for time series recording (1 ms exposure, further depending on Sensor ROI, hardware and software configuration) H x V (ROI) FrameRate (fps) 4512 x 4512 28 2256 x 2256 75 (2x2 subsampling; full field of view) 2248 x 1504 82 1920 x 1080 110 1024 x 1024 115 512x x 512 207 1920 x 256 346 1920 x 128 447 Read Noise Full Well Capacity Dynamic Range (at gain) 2.3 e (1x) 10,000 e 1:4440 1.8 e (2x) 5,500 e 1:2720 1.6 e (4x) 2,500 e 1:1540 1.5 e (8x) 1,250 e 1: 850 1.3 e (16x) 625 e 1: 470 HDR Mode: 1,6 e 40,000 e 1:25,000 Software Supported Camera Operation: Exposure time adjustment with continuous, automatic or manual mode, Adjustable over or under-exposure mode, Optional measurement on defined target area (spot-meter), Image sharpening, Dark current compensation, Single pixel noise correction Readout of Sensor Sub Regions ("ROI"): Adjustable Digitization: 8, 12, 14 bit / pixel (8 out of 14 bit or compression mode) Dark Current: <(><<)> 0.1 e/p/s @ 25 °C sensor temperature Cooling: Stabilized temperature to 25 °C (mounted on microscope, free air flow, ambient temp. 22 °C) Exposure Time Range: 0.1 ms to 60 s Hardware Trigger: Trigger-In for exposure timecontrol Trigger-Out for exposure time Readout time and trigger ready status (further accessories required) Status-LED for Camera: Color coded operation status, dimmable Camera Interface: Dual USB 3.0 SuperSpeed, 2 x 5 Gbit/s for data and power supply (optional single USB 3.0 at lower framerates and without cooling) OpticalInterface: C-Mount Thread Depth for Objectives: Max. 5 mm Size / Weight: Approx. 10.8 cm x 7.8 cm x 6.1 cm (4.2" x 3.1" x 2.4") / 620 g Housing: Blue anodized aluminum, with cooling fins on top 1/4" photo thread for tripod mount Registration: CE Power Supply: Provided by Dual USB 3.0, 5V, max. 7 W power consumption Environmental Conditions: 5 °C to 35 °C, max. 80% relative air humidity, not condensing, free air circulation required Supported Operating Systems: for ZEN: Windows 10 x64 Enterprise Supported Application Software: ZEN 3.6 (blue edition) or higher, ZEN 3.5 core or higher The required application software is provided via ZEISS Microscopy Installer or ZEISS Download Center.

Trigger Cable Set Axiocam 5xx/7xx/8xx (D) for direct shutter synchronization using trigger board with signal distribution box, Colibri or HXP 120 V

ZEN 3.9 Image acquisition and processing under Windows 10 x64 User interface configurable, control of the ZEISS microscope systems and components, extensive acquisition and analysis, CZI image format The following functions are included: - arivis cloud advanced - Basic 3D viewing - Colocalization - Connect basic - Direct processing - Manual EDF - Measurement - Panorama - Spectral unmixing - ZEN Data Storage Client - Acquisition Base (time series and multichannel)

ZEN Toolkit Motorized Acquisition Acquire images and control motorized components in multi-dimensional experiments. This package allows to acquire tiles, multi-position and z-stack images using motorized components. These can be combined with the included autofocus and direct processing. z-stack and enhanced depth of focus functionality: - definition of the Z-stack in "Start/Stop" and "Center" mode - size of the configurable Z-stack and number of possible Z-planes only limited by the travel range of the system and the minimum increment - "Optimal" button to automatically set the correct increment to satisfy the Nyquist criterion - integrated Z-drive backlash compensation for maximum precision and reproducibility - extraction of the sharp details from individual images at various focus positions and combination into an image with high depth of field Tiles <(>&<)> Positions - automatic scanning of predefined sample areas - acquisition of images according to a position list - support of focus correction maps, stitching and shading correction - compatible with software autofocus Software Autofocus: - determination of the optimal focus position in the sample - the autofocus works with transmitted, reflected and fluorescence light - definition of an autofocus region in the camera image

ZEN Toolkit 2D Create flexible automatic measurement programs for the analysis of your 2D images. Set up an automatic measurement program for analyzing your data sets (e.g., time series, tile images and multiscene images) in just a few steps. A user-friendly wizard guides you through all the necessary settings, starting with the definition of the classes and the segmentation of objects, to the selection of measurement parameters. Create simple measurement programs consisting of a single class or set up powerful measurement programs with multiple classes, which you can also arrange hierarchically (objects within objects) or use the predefined zone-of-influence* measurement program if you want to detect objects in the periphery of other objects. Flexibly choose between different classical and AI-based segmentation methods for each class. You can improve your results using AI models (semantic or instance segmentation) especially for datasets which are particularly difficult to segment. This toolkit is "AI-ready", which means you can use any trained AI model in the analysis program without requiring another license. Simply load a model previously trained with the ZEN Toolkit AI in ZEN or ZEN core into the Image Analysis Wizard, or use DNNs trained on arivis Cloud. Subsequently, you can further refine the segmentation results, e.g., by using algorithms for object separation or by applying region filters, if necessary. To quantify the detected objects, a variety of measurement features are available, such as different geometrical (size, shape,...) or intensity features as well as user-defined measurement parameters*. After the analysis, the segmented images are displayed along with result tables and diagrams (e.g., scatterplot or heatmaps). Tables, diagrams, and images are linked so that you can interactively explore your results. You can also use the created image analysis programs for batch processing of multiple data sets. In addition, this toolkit also includes the Bio App Cell Counting*. (*not available in ZEN core) For preprocessing of your image data, the toolkit additionally provides a number of special image processing functions belonging to the Edges (e.g. Highpass, Sobel, Laplace,…), Arithmetic (e.g. Addition, Multiply Constant,…), Morphology (e.g. Erode, Dilate, TopHat,…), Segmentation (e.g. Canny, Marr…) and Binary (e.g. Fill Holes, And, Or,…) Operations groups. Requirements: - The use of AI semantic segmentation requires an installation of Zeiss Python - The use of AI instance segmentation requires an installation of Docker Desktop For the use of AI models the recommended hardware configuration is 8GB GPU and 64GB RAM. Only the CPU and Nvidia GPUs are supported.

Dust protection Set L consisting of dust cover (L670xW460xH775 mm), eyepiece cover and protection cap for luminous-field diaphragm

Anti-vibration plate Axio Imager and LSM 800/900 (D) can be used to set up an Axio Imager or LSM 800/900 on undamped tables.