Axio Observer live Standard

Z-drive operation, flat; right Flat, ergonomic control knob on the right side, recommended for combinations with mechanical stage or object guides, right

Microscope stand Axio Observer 7 - motorized focus drive (min. step width 10 nm) - prepared for motorized beam path deflection - with optional support of laser based applications - TFT touchscreen - 1x tube lens to fit with optional available 1-position tube lens mount or 3-position optovar turret mot. - circular operation key unit right and left - keys for switching TL/RL illumination - light- and contrast manager - interfaces: 4x CAN, 2x USB-A, 1x USB-C and 1x TCP/IP - trigger socket (In/Out) for shutter - connecting socket for external UNIBLITZ shutter - external power supply 12V DC 100W, stabilized, 100V-240VAC / 50-60Hz / max. 190VA - USB cable and country-specific line cable

Anti vibration set Axio Observer (D) Comprising: 2 supporting plates, fitted with 2 vibration dampers (each), for the fixed mounting at the bottom of the Axio Observer stand

1-position tube lens mount, fixed

Sideport 60N L/R 100, 3 switching positions (100% vis : 0% LR / 0% vis : 100% L / 0% vis : 100% R)

Beam path switching mot between visual observation or frontport or baseport

6-position objective nosepiece, H DIC M27 mot.

Dual filter wheel mot. for beam splitting and emission; CAN with mount for 6x beam splitter and 8x emission filter for use instead of the reflector turret on Axio Observer.Z1 and Axio Observer 7. - usage connected with with CAN distributor (available separately) - incl. Power supply 100-240V / 50-60 Hz / max. 1.4 A; 24V DC / max. 2.5 A with country specific plug Beam splitter filter wheel: - mount for up to 6 beam splitters 36 x 26 mm - switching time of <(><<)> 300 ms for adjacent filters Emission filter wheel: - mount for up to 8 filters with d=25 mm - filter thickness max. 4 mm - switching time of <(><<)> 60 ms for adjacent filters

Docking station for TFT-Display upgrading module for the TFT-Display of motorized stands Axio Imager and Axio Observer - free positioning on desktop - Control of z-drive and circular operation key unit - mechanical interface to attach Electronic Coaxial Drive - TFT-Display 30°-45° tiltable

Illuminator VIS-LED for mounting on the transmitted-light, reflected-light illuminator, included LED lamp module for VIS-LED - color temperature 5600 K - average lifetime > 10,000 hours - control signals: Trigger-input (BNC) for controlling, max. trigger frequency ca. 100 Hz - usable for objectives from 2.5x - incl. neutral-density filter 0.12 D=25x2 for use with digital microscopy cameras

Carrier transmitted-light illumination with Shutter - for Axio Observer 7/Z1 - manual luminous-field diaphragm - shutter, switching time <(><<)> 80ms - condenser mount - interface for LD condenser 0.55 mot. - 3-position filter changer, d=32 mm - carrier tiltable

Reflected-light illuminator FL mot. - slider mounts for motorized and manual sliders - slider mount for 3-position filter slider, dia. 25 mm - beam path apochromatic corrected - transmission from 375 to 825 nm >80%

Solid-State Light Source Colibri 7, Type R[G/Y]B-UV 5-channel fluorescence light source with integrated control unit for continuous brightness adjustment, quickly switchable and stable on the long term. With integrated monitor diode for repetitious brightness adjustment. Equipped with 5 excitation lines. Red (630nm) for excitation of Cy5, Alexa 631, TOTO-3 and similar dyes Yellow (590nm) for excitation of mCherry, Alexa 568, mPlum and similar dyes Green (555nm) for excitation of Cy3, TRITC, DsRed and similar dyes Blue (475nm) for excitation of eGFP, Fluo4, FITC and similar dyes UV (385nm) for excitation of DAPI, Alexa 405, Hoechst 33258 and similar dyes A country-specific power cable and a desktop power supply are included in delivery.

4-position CAN hub for microscope stands with CAN interface for the connection of up to 4 components with CAN connector to a CAN interface on microscope stands Axio Observer 3 / 5 / 7 / D1 / Z1 and Axio Imager M1 / Z1, M2 / Z2 and Z2 Vario

Scanning Stage 130x100 STEP (D) for Axio Observer and Axiovert 200 - stepper motor with 2 mm spindle pitch - connection to stepper controller - travel range 130 mm x 100 mm - stage surface 325 mm x 144 mm - max speed 50 mm/s - resolution: 0.1 µm - reproducibility: +/- 1 µm - absolute accuracy: +/- 5 µm - travel range adjustable - fixed motor cable with exit on stage back side; left - for mounting frames K 160 x 110 mm and Z PIEZO - compatible with objectives autocorr

Universal mounting frame K for: - Petri dishes dia. 24...68 mm - slides (max. length 120 mm) frame size 160 x 110 mm

Binocular tube 45°/23 with manual shutter

Filter Set 90 LED SBP DAPI+GFP+DsRed+Cy5 for use with Colibri 5/7 and emission filter wheel Consisting of: BS 405 + 493 + 575 + 653 EM BP 425/30 EM BP 514/30 EM BP 592/25 EM BP 681/45

Filter set 92 HE LED (E) for use with the illumination system Colibri 7. Suitable for fluorescent dyes like DAPI, GFP and mCherry with excitation wavelengths 385, 475 and 590 nm. Contains beam splitter TBS 405 + 493 + 610 and emission filter TBP 425/30 + 524/50 + 688/145.

Camera Adapter 60N-C 1" 1.0x

Eyepiece PL 10x/23 Br. foc.

Eyepiece eyecup

Objective EC Plan-Neofluar 5x/0.16 M27 (FWD=18.5mm)

Objective Plan-Apochromat 20x/0.8 M27 (FWD=0.55mm), incl. Cover glasses, high performance, CG=0.17mm, box with 100 pc Lead-free

Objective Plan-Apochromat 40x/0.95 Corr M27 (FWD=0.25mm), incl. Cover glasses, high performance, CG=0.17mm, box with 100 pc.

CAN Distributor for Motorized Objectives and Filter Wheels for up to 3 objectives autocorr and 1-2 filter wheels

LD condenser 0.55 mot.; AI Sample Finder - with motor operated aperture diaphragm and 7 turret disc positions for H, Ph1, Ph2, Ph3, 2x DIC as well as additional position for autofocus and coherence contrast - LED array for contrast enhancement in the overview image - Camera module for overview image - Deflecting mirror for LD condenser 0.55 mot. AI Sample Finder - Calibration plate 160x110 AI Sample Finder - WD=26 mm - for objective magnifications 4,0x-100x

Stage Controller XY STEP SMC 2009 Control unit for Maerzhaeuser XY-Stages with stepper motors. - bidirectional communication to microscopes with CAN - position display on microscopes with TFT display and option for objective depended travel speed - 2 CAN interfaces - alternative connection through USB or RS232 - power supply 100...240V AC/50...60Hz - incl. USB-cable, CAN-cable and plug-in power unit with country specific power cord

Joystick XY; CAN (D) for mechanical stages mot; CAN, scanning stages; CAN and stage controller XY STEP SMC 2009 - 2 speed ranges selectable - direct cable connection to CAN stage or to stage controller XY STEP SMC 2009

Extension cable for stage control 1.6 m (D) for usage with joystick, trackball or coaxial drive. Recommended for adaptation of cable length when a docking station is used.

Heated plate K with 3 inserts, ibidi® (D) To be used with Temperature Controller, ibidi® - Suitable for mechanical and scanning stages with opening 160 x 110 mm - Including inserts for: - Slides and Chamber Slides (62...78 mm length to 26.5 mm width or 57...70 mm length to 27.5 mm width) - 35 mm Petri dishes (32 - 44 mm diameter, 9 - 14 mm height) - 60 mm Petri dishes (48 - 68 mm diameter, 9 - 14 mm height) - Temperature range: ambient to 45°C - Outer dimensions: 160 mm x 130 mm x 12 mm (W x D x H) ibidi® is a registered trademark of the ibidi GmbH

Glass Lid CO2/O2 heated, ibidi® (D) To be used with Temperature Controller, ibidi® - Suitable for ibidi® Heated Plate K with 3 inserts and ibidi® Mounting frame K for multiwell plates - Temperature range: ambient to 45°C - Compatible with DIC contrast - 4 openings for perfusion tubing (width 3 mm, height 7 mm) - Opening for humidity sensor (part of ibidi® Gas Mixer System) - Luer Lock Connector for heated gas tubing (ibidi® Gas Mixer System) - The Gasmixer System requires pressured air (1 bar) - Observation area: 142 mm x 70 mm - Outer dimensions: 160 mm x 110 mm x 19 mm (W x D x H) ibidi® is a registered trademark of the ibidi GmbH

Temperature Controller, ibidi® (D) - For temperature control of up to 4 electrically heated components: Glass Lid CO2/O2 heated, ibidi® Heated Plate K with 3 inserts, ibidi® Heating Unit XL (PeCon) Objective heater (PeCon) -includes Temperature Adjustment Set: -Type K thermocouple and 3 dishes for temperature adjustment (µ-Dish 35 and 50 mm, µ-Slide 8 well) Connections: - Input channel for thermocouple to control sample temperature - USB and CAN connection Software: - ibidi® IncuControl Resolution. 0.1°C Precision: +/- 0.5°C Temperature Control Range: Channel 1 (Heated Lid): Ambient temperature (min. 18°C) to +46°C Channel 2 (Heated Plate): Ambient temperature (min. 18°C) to +45°C - incl. power supply 100-240V / 50 - 60Hz; Output: max. 2A; 24V DC / max. 6.67A 160W - Dimension: 230 mm x 170 mm x 90 mm (W x D x H) ibidi® is a registered trademark of the ibidi GmbH

Gas Mixer System CO2, ibidi® (D) For CO2 control and humidification - Includes controller, humidifying column and humidity sensor to be mounted at Glass Lid CO2/O2 heated/unheated - Includes 2 x 5m gas tubing (PUN-H) - The Gas Mixer System requires pressured air (1 bar ) Software: - ibidi® IncuControl - CO2 regulation up to 15% - CO2: resolution 0.1%, precision +/- 0.35% at 5% CO2 - Humidity regulation: 20-99%, resolution 1%, precision +/- 5% - no manual calibration required Connections: - Input channel for humidity sensor for feedback regulation - USB- and CAN connection - External power supply 100-240V / 50-60Hz / max. 1.4 A; Output: 24V DC / max. A, 120W; with country specific plug - Dimensions controller: 170 mm x 230 mm x 90mm (W x D x H) - Dimensions humidifying column: 70 mm x 110 mm x 300 mm (W x D x H) ibidi® is a registered trademark of the ibidi GmbH

Language Pack English US (O) - Keyboard with English (US) layout

Network Adapter 2 x 10 GbE RJ45 (HP Z8 G5) (O) - Additional network adapter 2 x 10 GbE RJ45 (HP Z8 G5) e.g. for connection of storage systems - only for 410203-9914-000 Microscopy Workstation Premium HP Z8 Fury G5

Microscopy Workstation Premium (HP Z8 G5) (O) HP Z8 Fury G5 Workstation - Chipset: Intel W790 - Memory Expansion Slots: 16 x DDR5, RDIMM, ECC, 4800 MHz Modules - Memory: max. 1024 GB RAM - PCI Express Connectors: 2 x PCI Express Generation5 x16 1 x PCI Express Generation 5 x4 2 x PCI Express Generation 4 x16 2 x PCI Express Generation 4 x4 1 x PCI Express Generation 3 x8 - SSD: 1 x 2 TB M.2 NVMe - Hard Drives: 2 x 12 TB SATA 7200 rpm (configured as 10 TB RAID 1hard drive) - Network Controller: 2 x GbE LAN interface (RJ 45) - USB: 2 x USB 3.2 Gen2x2 type C (front), 8 x USB 3.1 Gen 1 type A (2 x front, 6 x rear) - Certifications <(>&<)> Declarations: CE, CSA, UL, CCC, KCC - Power Supply: 100 - 240 V / 50 - 60 Hz / 1450 W with country specific plug - Chassis Dimensions: (H x W x D): 445 x 216 x 555 mm - USB Mouse, Recovery kit

System Configuration 2 for HP Z8 G5 (O) Installation of Windows 10 IoT Enterprise LTSC 2021 x64 incl. Windows 10 License - with ZEN software installation and system integration of respective system - for Celldiscoverer 7, LSM 900, LSM 980, Elyra 7, Lattice SIM, Axioscan 7, Lattice Lightsheet - only for 410203-9914-000 Microscopy Workstation Premium HP Z8 Fury G5

Processor Intel Xeon W5-3425 (HP Z8 G5) (O) Default processor configuration - Intel Xeon W5-3425 (3.2 GHz, 30 MB cache, 12 cores) - only for 410203-9914-000 Microscopy Workstation Premium HP Z8 Fury G5

Memory 32 GB (1x32) DDR5 4800 MHz ECC reg RAM (HP Z8 G5) (O) consisting of 1 x 32 GB module - DDR5 4800 MHz ECC registered DIMM - can be extended to 64 GB (2 x 410303-3206-000), 128 GB (4 x 410303- 3206-000) or 256 GB (8 x 410303-3206-000) - only for 410203-9914-000 Microscopy Workstation Premium HP Z8 Fury G5

Graphics Card NVIDIA RTX A4000 16 GB DP (O) Professional graphics card for Microscopy Workstation - 16 GB GDDR6 memory - PCI Express 4.0 Interface x16 - Hardware acceleration of DirectX 12.07, OpenGL 4.68 and CUDA - Support for up to four displays - Display outputs: 4 x DisplayPort 1.4a (DP) - DisplayPort support for 4 x 32" Display (3840 x 2160 Pixel)

Trigger Cable Set Axiocam 5xx/7xx/8xx (D) for direct shutter synchronization using trigger board with signal distribution box, Colibri or HXP 120 V

Microscopy Camera Axiocam 807 mono (D) High performance microscopy monochrome camera incl. driver software 64bit, Interface card USB 3.1 Gen2 PCIe x4, dual USB 3.0 cable 3 m Sensor Type: CMOS, monochrome, Global Shutter, Sony IMX420 Number of Pixels: 3216 (H) x 2208 (V) = 7.1 Mega Pixels Pixel Size: 4.5 µm x 4.5 µm Chip Size: 14.5 mm x 9.9 mm, equivalent to 1.1" (17,6 mm diagonal) Spectral Range: With protection glass, 350 nm to 1000 nm Peak QE: 78% @ 520 nm (typ.) Full Well Capacity: Approx. 25,000 e- at gain 1x (typ.) Adjustable Signal Amplification: 1x, 2x, 4x, 8x, 16x Selectable Resolution: H x V Binning Factor (digital) 3216 x 2208 1x1 basic pixel count 1608 x 11042x2 1072 x 991 3x3 884 x 743 4x4 643 x 594 5x5 (at 2x2 combined analog and digital binning for further increase of sensitivity) H x V Subsampling Factor (full field of view) 3216x2208 1x1 1608 x 1104 2x2 Live frame rates (depending on hardware and software configuration): Exp. Time 1ms H x V Frame Rate 3216 x 2208 >30 fps (High Res mode) 1602 x 1104>30 fps (Fast Mode, reduced CPU load) Frame rates for time series recording (1 ms exposure, further depending on sensor ROI, hardware and software configuration) H x V (ROI) Frame Rate (fps) 3216 x 2208 73 1929 x 1080 145 1608 x 1104 260 (2x2 subsampling; full field of view) 1024 x 1024 151 512 x 512 282 1920 x 256 487 Read Noise Full Well Capacity Dynamic Range (at gain) 5.7 e (1x) 25,000 e 1:4420 4.6 e (2x) 12,500 e 1:2730 3.9 e (4x) 6,250 e 1:1610 3.4 e (8x) 3,125 e 1: 930 2.9 e (16x) 1,500 e 1: 530 Low Noise Mode: 4.0 e 25,000 e 1:25,000 SoftwareSupported Camera Operation: Exposure time adjustment with continuous, automatic or manual mode, Adjustable over or under-exposure mode, Optional measurement on defined target area (spot-meter), Image sharpening, Dark current compensation, Single pixel noise correction Readout of Sensor Sub Regions ("ROI"): Adjustable Digitization: 8, 12, 14 bit / pixel (8 out of 14 bit or compression mode) Dark Current: <(><<)> 0.3 e/p/s @ 25 °C sensor temperature Cooling: Stabilized temperature to 25 °C (mounted on microscope, free air flow, ambient temp. 22 °C) Exposure Time Range: 0.1 ms to 60 s Hardware Trigger: Trigger-In for exposure time control Trigger-Out for exposure time Readout time and trigger ready status (further accessories required) Status-LED for Camera: Color coded operation status, dimmable Camera Interface: Dual USB 3.0 SuperSpeed,2 x 5 Gbit/s for data and power supply (optional single USB 3.0 at lower frame rates and without cooling) Optical Interface: C-Mount Thread Depth for Objectives: Max. 5mm Size / Weight: Approx. 10.8 cm x 7.8 cm x 6.1 cm (4.2"x 3.1" x 2.4")/ 620 g Housing: Blue anodized aluminum, with cooling fins on top 1/4" photo thread for tripod mount Registration: CE Power Supply: Provided by Dual USB 3.0, 5V, max. 7 W powerconsumption Environmental Conditions: 5 °C to 35 °C, max.80% relative air humidity, not condensing, free air circulation required Supported Operating Systems: for ZEN: Windows 10 x64 Enterprise Supported Application Software: ZEN 3.6 (blue edition) or higher, ZEN 3.5 core or higher The required application software is provided via ZEISS Microscopy Installer or ZEISS Download Center.

ZEN 3.9 Image acquisition and processing under Windows 10 x64 User interface configurable, control of the ZEISS microscope systems and components, extensive acquisition and analysis, CZI image format The following functions are included: - arivis cloud advanced - Basic 3D viewing - Colocalization - Connect basic - Direct processing - Manual EDF - Measurement - Panorama - Spectral unmixing - ZEN Data Storage Client - Acquisition Base (time series and multichannel)

ZEN Toolkit Motorized Acquisition Acquire images and control motorized components in multi-dimensional experiments. This package allows to acquire tiles, multi-position and z-stack images using motorized components. These can be combined with the included autofocus and direct processing. z-stack and enhanced depth of focus functionality: - definition of the Z-stack in "Start/Stop" and "Center" mode - size of the configurable Z-stack and number of possible Z-planes only limited by the travel range of the system and the minimum increment - "Optimal" button to automatically set the correct increment to satisfy the Nyquist criterion - integrated Z-drive backlash compensation for maximum precision and reproducibility - extraction of the sharp details from individual images at various focus positions and combination into an image with high depth of field Tiles <(>&<)> Positions - automatic scanning of predefined sample areas - acquisition of images according to a position list - support of focus correction maps, stitching and shading correction - compatible with software autofocus Software Autofocus: - determination of the optimal focus position in the sample - the autofocus works with transmitted, reflected and fluorescence light - definition of an autofocus region in the camera image

ZEN Toolkit Bio Apps Easy-to-use image analysis for standard life science applications. Choose from the selection of Bio Apps one, that fits your application and simply adapt the preconfigured Bio App to your data if necessary. Use fast, classical segmentation methods or powerful AI models to detect your cells even more accurately. This toolkit is "AI-ready", meaning you can use previously trained AI models in the Bio Apps without needing another license. Simply load an AI model trained with the ZEN Toolkit AI or a DNN trained on apeer for semantic or instance segmentation in the BioApp. After analysis, the segmented images are displayed along with customized results tables and diagrams (e.g., heatmaps and scatterplots) so you can get a quick overview of your sample. At the same time, tables, charts, and images are interlinked so that you can explore the results interactively. All Bio Apps offer a heatmap view, making them particularly suitable for analyzing experiments in multi-well plates. They support both 2D endpoint and live cell assays. - Get started fast with Bio Apps tailored to specific applications - Available Bio Apps: Cell counting, confluency, gene- and protein expression and automated spot detection - Easy operation with predefined parameters - Choose between fast classical segmentation or more powerful AI-models: use AI models trained with the ZEN Toolkit AI or DNNs trained on apeer with semantic or instance* segmentation (*not Confluency) - Visualize your results with dedicated and interactive result displays (table, heatmap, timeseries, xy-scatterplot, histogram) - Export your results, plots, and processing settings - Supports 2D fixed timepoint and time-series analysis Requirements: - The use of AI semantic segmentation requires an installation of Z Zeiss Python. - The use of AI instance segmentation requires an installation of Docker Desktop For the use of AI models the recommended hardware configuration is 8GB GPU and 64GB RAM. Only CPU and Nvidia GPUs are supported.

Dust cover for Axio Observer / Axiovert 200/200M (L600xB420xH620 mm)