Axiovert 7

Fine drive knob with scale, changeable for Axio Imager 2, Axio Lab.A1 and Axiolab 5

Microscope stand Axiovert 7 TL FL with motorized z-drive - 6-position nosepiece BD DIC M27, encoded with 6x20 mm slider mount - 6-position reflector turret, encoded for P&C modules - 12x35 mm mount for analyzer slider - Reflected-light illumination with interface for Viluma 5/7 / Colibri 3 / HBO / HXP / X-Cite Xylis II®, slider mounts for luminous field diaphragm, aperture stop, polarizer and filter slider - Carrier transmitted-light with integrated white LED 10W (average LED lifetime > 60000 hrs) and 2-position filter slider, d=32 mm with phosphorescence block filter - motorized focus drive (resolution 78 nm) - 13 mm focus lift with adjustable focus stop - TL/RL switching - snap button for ZEISS Axiocam - ECO mode - Lightmanager for TL LED and Colibri 3 - CAN port for Colibri 3 - Parfocality manager - USB 2.0, Type-B interface to PC - external power adaptor input: 100-240V AC, 50/60Hz output: 24V DC, 5A - country specific power cable - Dust protection set

Slider with Mirror 100% for Intermediate Phototube - 2 clickstop positions for 100vis/100doc

Intermediate phototube H=50mm, left port - Camera port with interface 60N - Slider mount with 2 clickstop positions Additionally required: - Slider with mirror 100% - Slider with beam splitter 50% - Slider with beam splitter 20/80%

Stop slider A 14x40 mm with aperture/luminous field diaphragm - aperture diaphragm for Axio Scope - luminous field diaphragm for Axiovert 5/7 , Axio Vert.A1

llumination adapter A, achromatic - for Axiovert 5/7 and Axio Vert.A1 - for achromatization of the reflected light beam path - especially recommended for fluorescence microscopy with shorter excitation wavelengths

Light Source Viluma 5 RGB-UV 4-channel fluorescence light source with integrated control unit for continuous brightness adjustment, quickly switchable. Equipped with 4 solid state LED lamps. Red (631 nm) for excitation of Cy5, Alexa 631, TOTO-3 and similar dyes Green (555 nm) for excitation of Cy3, TRITC, DsRed and similar dyes Blue (469 nm) for excitation of eGFP, Fluo4, FITC and similar dyes UV (385 nm) for excitation of DAPI, Alexa 405, Hoechst 33258 and similar dyes A country-specific power cableand a desktop power supply are included in delivery. When used at an Axio Observer with Incubator XLmulti, the Extension tube for illuminator mount, 17 mm is required.

Control Panel for Colibri 5/7 and Viluma 5/7 with LCD to display the operating state. For LED selection and brightness setting.

Mechanical stage 130x85 R/L for Axiovert - for Axiovert 5 and Axio Vert.A1 - manual 3-plate mechanical stage - for mounting frames K 160 x 110 mm - max. 3 kg sample load

Universal mounting frame K for: - Petri dishes dia. 24...68 mm - slides (max. length 120 mm) frame size 160 x 110 mm

Universal mounting frame K-X for: - multitest dishes with footprint 124...133 x 83...88 mm - Petri dishes dia. 87...92 mm frame size 160 x 110 mm

Binocular tube 45°/23 - upright and unreversed image

Conversion filter 5700-3200 K, d=32 mm for adapting the LED light to the color impression of halogen light

Neutral-density filter 0.06, d=32x2

Filter Set 43 HE with Reflector Module FL EC P<(>&<)>C (E) Filter Set 43 HE shift free consisting of filters EX BP 550/25, BS FT 570, EM BP 605/70 inserted in reflector module, reflection avoided through tilted mount for emission filters. Recommended for white light fluorescence light sources or ZEISS LED fluorescence light sources.

Filter Set 90 HE LED with Reflector Module FL EC P<(>&<)>C (E) Filter Set 90 HE LED shift free for use with ZEISS LED fluorescence light sources with excitation wavelengths 385, 475, 555 and 630 nm. Consisting of filters QBS 405 + 493 + 575 + 653 and QBP 425/30 + 514/30 + 592/25 + 709/100 inserted in reflector module, reflection avoided through tilted mount for emission filters.

Filter Set 38 HE with Reflector Module FL EC P<(>&<)>C (E) Filter Set 38 HE shift free consisting of filters EX BP 470/40, BS FT 495, EM BP 525/50 inserted in reflector module, reflection avoided through tilted mount for emission filters. Recommended for white light fluorescence light sources or ZEISS LED fluorescence light sources.

Camera Adapter 60N-C 2/3" 0.63x

Eyepiece PL 10x/23 Br. foc. With mount for reticle diam. 26 mm.

Eyepiece eyecup

Objective LD A-Plan 5x/0.15 Ph1 M27 (FWD=11.7mm at CG=1mm polystyrene)

Objective LD A-Plan 10x/0.25 Ph1 M27 (FWD=8.5mm at CG=1mm polystyrene)

Objective LD A-Plan 20x/0.35 Ph2 M27 (FWD=4.9mm at CG=1mm polystyrene)

Objective LD A-Plan 40x/0.55 Ph2 M27 (FWD=2.3mm at CG=1mm polystyrene)

LD condenser 0.4 for slider - for H, Ph, PlasDIC - rotatable, with click stops 0°, 90°, 180°, 270° - for objectives 5x - 63x (2.5x with diffusion disk 2.5x) - 10x46 mm slider mount - 53 mm working distance - aperture stop

Slider 10x46 mm Ph/PlasDIC, H, Ph/PlasDIC - 2 positions for Ph- or PlasDIC diaphragms - brightfield position with neutral-density filter 0.06

Phase stop Ph 2/0.4 for slider

Phase stop Ph 1/0.4 for slider

Microscopy Camera Axiocam 305 mono (D) Microscopy camera incl. driver software 64bit, USB 3.0 PCIe x1 interface, USB 3.0 cable 3 m and BK7 protection glass (coated) Number of Pixels: 2464 (H) x 2056 (V) = 5.07 Mega pixel color Sensor type: SONY IMX264 Exmor Pregius Global Shutter CMOS Pixel size: 3.45 µm x 3.45 µm Chip size: 8.5 mm x 7.1 mm, equivalent to diagonal 11.1 mm (2/3" form factor) Spectral range: app. 350 nm to 1000 nm Full Well Capacity: Approx. 10,500 e Selectable Resolution: H x V Binning Factor 2464 x 2056 1x1 Basic Resolution 1232 x 1028 2x2 816 x 684 3x3 608 x 514 4x4 480 x 410 5x5 H x V Subsampling Factor 2464 x 2056 1x1 Basic Resolution 1232 x 1028 2x2 Live frame rates (depending on hardware and software configuration): H x V Frame Rate@1ms 2464 x 2056 36 fps 1232 x 1028 88 fps (2x2 Subsampling) Frame rates for time series recording (depending on computer hardware and software configuration) H x V (ROI) Frame Rate 2464 x 2056 36 fps 2048 x 2048 36 fps 1920 x 1080 67 fps 1024 x 1024 70 fps 512 x 512 136 fps 256 x 256 255 fps 128 x 128 456 fps Readout of Sensor Sub-Regions ("ROI"): Adjustable Digitization: 8 and 12 Bit / Pixel Dynamic Range: Typical > 4800: 1 (>73 dB) at typ. 2.2 e readout noise Dark current: <(><<)> 1.0 e/p/s @ 25°C Integration Time: 0.1 ms to 4 s Cooling: Temperature stable @ 25°C for ambient temperatures between 18°C and 30°C Status-LED for Camera: color coded operation status, dimmable Interface (camera): USB 3.0 interface, Optional operation with USB 2.0 connection at reduced frame rate Optical Interface: C-Mount Thread depth for objectives: max. 5 mm Size / Weight: approx. App. 10.8 cm x 7.8 cm x 4.3 cm (2.3"x3.2"x1.7") / 580 g Housing: blue anodized aluminum, with cooling fins on top, 1/4" photo thread for tripod mount Registration: CE Power Supply: Max. 4W, power supply provided by USB 3.0 bus for camera electronics Environmental conditions: 5° C +35° Celsius, max 80% relative air humidity, not condensing, free air circulation required Supported Operating Systems: for ZEN: Windows 10 x64 for Labscope: Windows 10 x64 Supported Application Software: ZEN 2.6 (blue edition) or higher, ZEN core v2.7 or higher Labscope v3.3 (Windows) or higher The required application software is available via the ZEISS Download Center.

ZEN 3.14+ Software platform for image acquisition and processing on Windows 11 User interface configurable, control of the ZEISS microscope systems and components, extensive acquisition and analysis, CZI image format The following functions are included: - Basic 3D viewing - Colocalization - Connect basic - Direct processing - Manual EDF - Measurement - Panorama - Spectral unmixing - ZEN Data Storage Client - Basic acquisition functions (time series and multichannel) - ZEN API Gateway The license entitles to use the ZEN software in the specified version AD in the next ZEN version. Includes a Docker Desktop license required by selected ZEN toolkits.

ZEN Toolkit Motorized Focus Acquisition 3.14+ This toolkit allows to acquire a z-stack image and images with enhanced depth of field on systems with motorized z-drive. These acquisitions can be combined with the included software autofocus. Z-stack and enhanced depth of focus functionality: - definition of the z-stack in "Start/Stop" and "Center" mode - size of the configurable Z-stack and number of possible Z-planes only limited by the travel range of the system and the minimum increment - "Optimal" button to automatically set the correct increment to satisfy the Nyquist criterion - integrated Z-drive backlash compensation for maximum precision and reproducibility - extraction of the sharp details from individual images at various focus positions and combination into an image with high depth of field Software Autofocus: - determination of the optimal focus position in the sample - autofocus works with transmitted, reflected and fluorescence light - definition of an autofocus region in the camera image The license entitles to use the ZEN software in the specified version AND in the next ZEN version.

ZEN Toolkit Deconvolution Sharpen your images with mathematically exact deconvolution and make previously unknown structures visible by increasing your image contrast and resolution. Execute the underlying iterative 3D DCV algorithms by leveraging multi-GPU in just minutes. You have the choice between preset parameters and free parameter selection in expert mode.