Microscope Axio Imager.M2m complete system for NMI

Axio Imager.M2m microscope stand with Z-drive mot. and TFT monitor - Z-drive mot. basic, stepsize 25 nm, with fine drive knob and fine drive disk, flat with scala, changeable - reflected-light illumination mot. with shutter and diffusion disk - 6-position reflector turret mot. - Light Control mot. - main controller incl. USB-A, USB-C and TCP/IP interface - external 12V DC 100W power unit, stabilized, 100V...240V AC/ 50...60Hz/230VA - USB cable and country-specific line cable

7-position objective nosepiece, HD M27 mot.

Transmitted-light/reflected-light stage carrier, detachable for Axio Imager suitable for sample heights from 0 mm up to 45 mm

Double filter wheel, discrete, mot., 2x4 positions each to accept neutral-density filter set and dia. 25 mm color filters. For use in transmitted-light and reflected-light equipments mot.

Stop slider mot. with centerable luminous-field diaphragm

Stop slider mot. with centerable aperture stop

Reflector module bright field ACR P&C for reflected light

Illuminator microLED 3 for mounting on the lamp interface for transmitted-light or reflected-light illumination - colour temperature 5700 K - colour rendering index >90

Compensator mount 6x20 with DF stop

Mechanical Stage 75x50 mot; CAN (D) for Axio Imager and SteREO - hard coating, linear encoders - stepper motor drive with integrated controller - connection via CAN bus - travel range 75 mm x 50 mm - max speed 100 mm/s - resolution: 0.1 µm - reproducibility: +/- 1 µm - absolute accuracy: +/- 5 µm - max sample weight: 1 kg - incl. CAN cable

Specimen holder, reflected light, for mechanical stage 75x50 plate dimension 120x107

Electronic Coaxial Drive; CAN (D) for mechanical stages mot; CAN, scanning stages; CAN and stage controller XY STEP SMC 2009 - mounted to lower stage plate of stages CAN or docking station - ergonomic use - direct cable connection to CAN stage or stage controller XY STEP SMC 2009

Binocular phototube 15°/25 (100:0/0:100), upright image adjustable stop, camera port with interface 60N

Conversion filter 5700-3200 K, d=25 mm for adapting the LED light to the color impression of halogen light

Neutral-density filter set for reflected light, d=25 consisting of 7 neutral-density filters with 2x 50%, 25%, 3x 12% and 3% transmission, for integration in filter wheels, discrete, man. or mot., for the use at strongly reflective samples with HAL 100 illuminator, also suitable for HBO/XBO

Camera Adapter 60N-C 2/3" 0.63x; rotating +/- 2° for fine adjustment of camera rotation. - adjustment range: +/- 2° - maximum weight load: 2 kg

Stage micrometer for reflected light 5 + 100/100y d=0mm - 5 lines with 1mm intervals in y-direction - 100 lines with 1/100mm intervals in y-direction

Eyepiece PL 10x/23 Br. foc.

Eyepiece eyecup

Objective EC Epiplan-Neofluar 20x/0.50 BD DIC M27 (FWD=2.2mm)

Objective EC Epiplan-Neofluar 10x/0.25 BD DIC M27 (FWD=9.0mm)

Objective EC Epiplan-Neofluar 5x/0.13 BD DIC M27 (FWD=14.5mm)

Microscopy Workstation Premium (HP Z8 G5) (O) HP Z8 Fury G5 Workstation - Chipset: Intel W790 - Memory Expansion Slots: 16 x DDR5, RDIMM, ECC, 4800 MHz Modules - Memory: max. 1024 GB RAM - PCI Express Connectors: 2 x PCI Express Generation5 x16 1 x PCI Express Generation 5 x4 2 x PCI Express Generation 4 x16 2 x PCI Express Generation 4 x4 1 x PCI Express Generation 3 x8 - SSD: 1 x 2 TB M.2 NVMe - Hard Drives: 2 x 12 TB SATA 7200 rpm (configured as 10 TB RAID 1hard drive) - Network Controller: 2 x GbE LAN interface (RJ 45) - USB: 2 x USB 3.2 Gen2x2 type C (front), 8 x USB 3.1 Gen 1 type A (2 x front, 6 x rear) - Certifications <(>&<)> Declarations: CE, CSA, UL, CCC, KCC - Power Supply: 100 - 240 V / 50 - 60 Hz / 1450 W with country specific plug - Chassis Dimensions: (H x W x D): 445 x 216 x 555 mm - USB Mouse, Recovery kit

Processor Intel Xeon W5-3425 (HP Z8 G5) (O) Default processor configuration - Intel Xeon W5-3425 (3.2 GHz, 30 MB cache, 12 cores) - only for 410203-9914-000 Microscopy Workstation Premium HP Z8 Fury G5

Memory 32 GB (1x32) DDR5 4800 MHz ECC reg RAM (HP Z8 G5) (O) consisting of 1 x 32 GB module - DDR5 4800 MHz ECC registered DIMM - can be extended to 64 GB (2 x 410303-3206-000), 128 GB (4 x 410303- 3206-000) or 256 GB (8 x 410303-3206-000) - only for 410203-9914-000 Microscopy Workstation Premium HP Z8 Fury G5

Graphics Card NVIDIA RTX A2000 12 GB (O) Professional graphics card for Microscopy Workstation - 12 GB GDDR6 memory - PCI Express 4.0 Interface x16 - Hardware acceleration of DirectX 12.07, OpenGL 4.68 and CUDA - Support for up to four displays - Display outputs: 4 x MiniDisplayPort 1.4 (mDP) - DisplayPort support for 4 x 32" Display (3840 x 2160 Pixel)

System Configuration 2 for HP Z8 G5 (O) Installation of Windows 10 IoT Enterprise LTSC 2021 x64 incl. Windows 10 License - with ZEN software installation and system integration of respective system - for Celldiscoverer 7, LSM 900, LSM 980, Elyra 7, Lattice SIM, Axioscan 7, Lattice Lightsheet - only for 410203-9914-000 Microscopy Workstation Premium HP Z8 Fury G5

Language Pack English US (O) - Keyboard with English (US) layout

Monitor TFT 24" HP Z24n G3 (61 cm) (O) Visible diagonal: 61 cm, 16:10 Maximum resolution: 1920 x 1200 pixel @ 60 Hz Brightness: 350 nits Contrast: 1000:1 Viewing angle: horizontal 178 degree, vertical 178 degree Video input: DisplayPort 1.2, HDMI 1.4 Data connectors: 1 x USB Type B (upstream), 4 x SuperSpeed USB Type-A 5Gbps Power consumption: Maximum 63 W, Typical 28 W, Standby 0.5 W Dimensions incl. stand (H x W x D): 52.1 cm x 53.2 cm x 19.5 cm Weight incl. stand: 4.9 kg Ergonomic features:Tilt: -5°to +20°, Swivel: -45° to +45°, Height adjustable 15 cm, Pivot rotation: -90° to +90° Certification and compliance: CE, CB, GS, TÜV, FCC, TCO Operating voltage: 100-240 V, country-specific power cable

Microscopy Camera Axiocam 705 color R2 (D) High Performance microscopy camera incl. driver software 64 bit USB 3.0 PCIe x1 interface, USB 3.0 cable 3 m and IR barrier filter (coated) Sensor Type: SONY IMX250 Exmor CMOS, SONY Pregius Global Shutter Color filter array Number of Pixels: 2464 (H) x 2056 (V) = 5.07 Mega pixel Pixel Size: 3.45 µm x 3.45 µm Chip Size: 8.5 mm x 7.1 mm, equivalent to 2/3" (diagonal 11.1 mm) Spectral Range: With IR barrier filter approx. 400 nm to 720 nm Full Well Capacity: Approx. 11,000 e at gain 1x (typ.) Adjustable Signal Amplification: 1x, 2x, 4x, 8x, 16x Selectable Resolution (color and monochrome): H x V Binning Factor (1x1 to 5x5 digital) 2464 x 2056 1x1 Basic Resolution 1232 x 1028 2x2 816 x 684 3x3 608 x 514 4x4 480 x 410 5x5 (at 2x2 combined analog and digital binning for further increase of sensitivity) H x V Subsampling Factor 2464 x 2056 1x1 1232 x 1028 2x2 Live Frame Rate: (1 ms exposure, default setting Fast Mode) H x V Frame Rate (fps) 1232 x 1028 30 Frame Ratesfor TimeSeries Recording: (1 ms exposure, further depending on hardware and software configuration) H x V (ROI) Frame Rate (fps) 2464 x 2056 60 1920 x 1080 115 1232 x 1028 173 (2x2 subsampling, full field of view) 1024 x 1024 121 512 x 512 235 1920 x 256 436 Read Noise Full Well Capacity Dynamic Range (at gain) 2.20e (1x) 11,000 e 1:5000 1.74 e (2x) 5,000 e 1:3100 1.48 e (4x) 2,700 e 1:1800 1.29 e (8x) 1,300 e 1:1300 1.15 e (16x) 690 e 1: 600 HDR Mode Dynamic Range 1:25,000 Software Supported Camera Operation: Exposure time adjustment with continuous, automatic or manual mode, adjustable over or under-exposure mode, optional measurement on defined target area (spot-meter), adjustable color saturation, White balance adjustment interactive or automatic mode with selectable target white balance color (warmer / colder) Readout of Sensor Sub Regions ("ROI"): Adjustable Digitization: 8, 12, 14 bit / pixel (8 out of 14 bit or compression mode) Dark Current: <(><<)> 0.5 e/p/s @ 18 °C sensor temperature Cooling: Stabilized temperature to 18 °C (for ambient temperatures from 18 °C to 30 ° C) Exposure Time Range: 0.1 ms to 60 s Hardware Trigger: Trigger-In for exposure time control, Trigger-Out for exposure time, Readout time and trigger ready status (further accessories required) Status-LED for Camera: Color coded operation status, dimmable Camera Interface: USB 3.0 (Optional operation with USB 2.0 at reduced frame rate) Optical Interface: C-Mount Thread Depth for Objectives: max. 5 mm Size / Weight: Approx. 10.8 cm x 7.8 cm x 5,1 cm (2.3" x 3.2" x 2,0") / 580 g Housing: Blue anodized aluminum, with cooling fins on top, 1/4" photo thread for tripod mount Registration: CE Power Supply: Provided by USB 3.0 and USB 2.0, max. 7 W consumption Environmental Conditions: 5 °C to 35 °C, max. 80% relative air humidity, not condensing, free air circulation required Supported Operating Systems: for ZEN: Windows 10 x64 Supported Application Software: ZEN 3.6 (blue edition, Hotfix 3.6.6) or higher, ZEN core 3.5 (Hotfix 3.5.4) or higher The required application software is available via the ZEISS Download Center.

ZEN core 3.9 Software suite for microscope image acquisition, processing, analysis, and data connectivity. - Configurable graphical user interface including workbench concept for customized routine jobs and workflows - Full integration of the ZEISS microscopes (incl. Visioner 1) that can be configured in MicroToolbox, Axiocam cameras and additional components - Interactive and automatic control of the individual motorized microscope components and transfer of information from encoded components into the software - Image acquisition with b/w, rgb, high-resolution and high-sensitivity cameras - HDR, Panorama, and manual Extended Depth of Focus (EDF) acquisitions - Basic image acquisition on electron microscopes - Image processing: standard operations for image optimization (contrast, brightness, gamma, colors, smoothing, sharpening, geometric corrections...) - Interactive/dimensional measurements, including online measurements on the live image - Input Form Designer with automated customer data import - Report creation and modification of Microsoft Word report templates using ZEN Word add-in - Data Archive for managing and maintaining documents: searching, filtering, tagging, viewing, re-opening, structuring, exporting, importing (any file format via drag n drop) - User Management for multi-user environment incl. full integration into the Windows multi-user functionality - Connectivity to ZEN Data Storage image database - Contains ZEN Connect basic functionality, 2D CAD overlay, topography viewer for 2.5D visualization of EDF Images incl. profile line measurement, QUAL Data Export - arivis Cloud connector enables the execution of customized solutions by integrating modules from the arivis Cloud platform Supported operating systems: Windows 10 (64-Bit)

ZEN Module Non-Metallic Inclusion Analysis Software Module for evaluation and rating of non-metallic inclusions in steel (Oxides, Sulfides and Nitrides). The following standards are supported: ASTM E45a, ISO 4967, JIS G0555, GB/T 10561, EN 10247, SEP 1571 and DIN 50602. The scope of delivery includes preconfigured and automated workflows for steel cleanliness analysis and -rating. The provided job templates are available for the user roles Supervisor and Operator and cover image acquisition, result presentation in interactive views for fast inspection and revision of inclusions, single reports with results of characteristic values and standard specific methods per mounted specimen. Furthermore, an overview report with the summary of the characteristic values, statistics and method results per standard for a set of evaluated specimens. Automated storage of generated results and inspection views in the data archive. Archived data can be re-opened and viewed. Optimized workflows for mounted specimen with/without nitride inclusions. The workflows can be conducted with acquired or loaded images. - Adjustable image acquisition settings for microscope and camera for the supervisor. Selectable image acquisition settings for the operator - Standard-measuring-field-based definition of the tiles acquisition area - Standard Template Editor to customize existing standards by copy and edit. Adjustable parameter: minimum particle and inclusion size; particle distance (in direction/perpendicular to main deformation orientation; size ratio for differentiation of globular and elongated inclusions. - Automated parallel analysis of all selected standards - Efficient auto segmentation for oxide, sulfide and nitride detection - Region adaption algorithm for optimized detection of mixed type Oxide/sulfide inclusions (complex, heterogenous inclusions) - Artifact filter for automated filtering of scratches - Advanced artifact filter for automated filtering of sulfide halos - Automated detection of the main deformation orientation (horizontal, vertical) - Standard specific severity rating filter: automated exclusion of inclusions per defined size class - Global result view with inclusion gallery for inspection and revision of objects (oxides, sulfides, nitrides and artifacts), several filter- and sorting options and result statistics. Standard results selectable by one mouse click. - Field based result view with acquired specimen image, a grid of standard-measuring-fields, specimen navigation for live inspection and revision of detected objects (oxides, sulfides, nitrides and artifacts), several filter- and sorting options per standard specific inclusion types and result statistics with field rating. Interactive selection of multiple objects for artifact classification. - Field based result view with True Worst Field detection: identification of inclusion type specific worst fields that are completely independent of the grid fields. - Standard results selectable by one mouse click. - Joint result table with characteristic core values of all selected standards. - Reports in Microsoft Word (from Microsoft Word 2013) and export as PDF. Automated generation of reports and summary reports for all standards. - Excel export of result tables - Customer database in Excel table format for input forms.

ZEN Toolkit Motorized Acquisition Acquire images and control motorized components in multi-dimensional experiments. This package allows to acquire tiles, multi-position and z-stack images using motorized components. These can be combined with the included autofocus and direct processing. z-stack and enhanced depth of focus functionality: - definition of the Z-stack in "Start/Stop" and "Center" mode - size of the configurable Z-stack and number of possible Z-planes only limited by the travel range of the system and the minimum increment - "Optimal" button to automatically set the correct increment to satisfy the Nyquist criterion - integrated Z-drive backlash compensation for maximum precision and reproducibility - extraction of the sharp details from individual images at various focus positions and combination into an image with high depth of field Tiles <(>&<)> Positions - automatic scanning of predefined sample areas - acquisition of images according to a position list - support of focus correction maps, stitching and shading correction - compatible with software autofocus Software Autofocus: - determination of the optimal focus position in the sample - the autofocus works with transmitted, reflected and fluorescence light - definition of an autofocus region in the camera image

Dust protection Set L consisting of dust cover (L670xW460xH775 mm), eyepiece cover and protection cap for luminous-field diaphragm