Axio Observer live Advanced

Z-drive operation, flat; right Flat, ergonomic control knob on the right side, recommended for combinations with mechanical stage or object guides, right

Microscope stand Axio Observer 7 - motorized focus drive (min. step width 10 nm) - prepared for motorized beam path deflection - with optional support of laser based applications - TFT touchscreen - 1x tube lens to fit with optional available 1-position tube lens mount or 3-position optovar turret mot. - circular operation key unit right and left - keys for switching TL/RL illumination - light- and contrast manager - interfaces: 4x CAN, 2x USB-A, 1x USB-C and 1x TCP/IP - trigger socket (In/Out) for shutter - connecting socket for external UNIBLITZ shutter - external power supply 12V DC 100W, stabilized, 100V-240VAC / 50-60Hz / max. 190VA - USB cable and country-specific line cable

1-position tube lens mount, fixed

Sideport 60N L/R 100, 3 switching positions (100% vis : 0% LR / 0% vis : 100% L / 0% vis : 100% R)

Beam path switching mot between visual observation or frontport or baseport

Anti vibration set Axio Observer (D) Comprising: 2 supporting plates, fitted with 2 vibration dampers (each), for the fixed mounting at the bottom of the Axio Observer stand

Definite Focus 3 incl. 6-position nosepiece H DIC M27 mot. ACR for Axio Observer 7 Consisting of: - sensor module Definite Focus 3 - Focus Controller 3 - slider mount for beam combiner module - attenuation filter BG 39, tilted, for LD condenser 0.55 - connecting cable and CAN cable

Beam Combiner Module UV/VIS for Definite Focus or all applications that require a transmission range between 320nm and 800nm.

Dual filter wheel mot. for beam splitting and emission; CAN with mount for 6x beam splitter and 8x emission filter for use instead of the reflector turret on Axio Observer.Z1 and Axio Observer 7. - usage connected with with CAN distributor (available separately) - incl. Power supply 100-240V / 50-60 Hz / max. 1.4 A; 24V DC / max. 2.5 A with country specific plug Beam splitter filter wheel: - mount for up to 6 beam splitters 36 x 26 mm - switching time of <(><<)> 300 ms for adjacent filters Emission filter wheel: - mount for up to 8 filters with d=25 mm - filter thickness max. 4 mm - switching time of <(><<)> 60 ms for adjacent filters

Docking station for TFT-Display upgrading module for the TFT-Display of motorized stands Axio Imager and Axio Observer - free positioning on desktop - Control of z-drive and circular operation key unit - mechanical interface to attach Electronic Coaxial Drive - TFT-Display 30°-45° tiltable

Illuminator VIS-LED for mounting on the transmitted-light, reflected-light illuminator, included LED lamp module for VIS-LED - color temperature 5600 K - average lifetime > 10,000 hours - control signals: Trigger-input (BNC) for controlling, max. trigger frequency ca. 100 Hz - usable for objectives from 2.5x - incl. neutral-density filter 0.12 D=25x2 for use with digital microscopy cameras

Carrier transmitted-light illumination with Shutter - for Axio Observer 7/Z1 - manual luminous-field diaphragm - shutter, switching time <(><<)> 80ms - condenser mount - interface for LD condenser 0.55 mot. - 3-position filter changer, d=32 mm - carrier tiltable

Reflected-light illuminator FL mot. - slider mounts for motorized and manual sliders - slider mount for 3-position filter slider, dia. 25 mm - beam path apochromatic corrected - transmission from 375 to 825 nm >80%

Solid-State Light Source Colibri 7, Type FR-R[G/Y]BV-UV 7-channel fluorescence light source with integrated control unit for continuous brightness adjustment, quickly switchable and stable on the long term. With integrated monitor diode for repetitious brightness adjustment. Equipped with 7 excitation lines. Far Red (735nm) for excitation of Cy7 and similar dyes Red (630nm) for excitation of Cy5, Alexa 631, TOTO-3 and similar dyes Yellow (590nm) for excitation of mCherry, Alexa 568, mPlum and similar dyes Green (555nm) for excitation of Cy3, TRITC, DsRed and similar dyes Blue (475nm) for excitation of eGFP, Fluo4, FITC and similar dyes Violet (430nm) for excitation of eCFP, Lucifer Yellow, Alexa 430 and similar dyes UV (385nm) for excitation of DAPI, Alexa 405, Hoechst 33258 and similar dyes A country-specific power cable and a desktop power supply are included in delivery.

4-position CAN hub for microscope stands with CAN interface for the connection of up to 4 components with CAN connector to a CAN interface on microscope stands Axio Observer 3 / 5 / 7 / D1 / Z1 and Axio Imager M1 / Z1, M2 / Z2 and Z2 Vario

Scanning Stage 130x100 STEP (D) for Axio Observer and Axiovert 200 - stepper motor with 2 mm spindle pitch - connection to stepper controller - travel range 130 mm x 100 mm - stage surface 325 mm x 144 mm - max speed 50 mm/s - resolution: 0.1 µm - reproducibility: +/- 1 µm - absolute accuracy: +/- 5 µm - travel range adjustable - fixed motor cable with exit on stage back side; left - for mounting frames K 160 x 110 mm and Z PIEZO - compatible with objectives autocorr

Universal mounting frame K for: - Petri dishes dia. 24...68 mm - slides (max. length 120 mm) frame size 160 x 110 mm

Binocular tube 45°/23 with manual shutter

Filter Set 91 LED SBP CFP+YFP+AF594 for use with Colibri 5/7 and emission filter wheel Consisting of: BS 450 + 538 + 610 EM BP 467/24 EM BP 558/25 EM BP 649/65

Filter Set 112 LED SBP DAPI+GFP+DsRed+Cy5+Cy7 for use with Colibri 5/7 and emission filter wheel Consisting of: BS 405 + 493 + 575 + 654 + 761 EM BP 425/30 EM BP 514/30 EM BP 592/25 EM BP 681/45 EM BP 788/38

Camera Adapter 60N-C 1" 1.0x

Eyepiece PL 10x/23 Br. foc.

Eyepiece eyecup

Objective EC Plan-Neofluar 5x/0.16 M27 (FWD=18.5mm)

Objective LD LCI Plan-Apochromat 40x/1.2 Imm Corr DIC M27 for water, silicon oil or glycerine immersion (CG=0.15-0.19mm) (FWD=0.41mm at CG=0.17mm) incl. Immersol G, bottle 20 ml and Cover glasses, high performance, CG=0.17mm, box with 100 pc

Objective Plan-Apochromat 10x/0.45 M27 (FWD=2.1mm)

Objective Plan-Apochromat 20x/0.8 M27 (FWD=0.55mm), incl. Cover glasses, high performance, CG=0.17mm, box with 100 pc Lead-free

Objective ring ACR for objective sleeve cylindrical short

Objective ring ACR for objective sleeve conical short

Objective ring ACR for objective sleeve conical long

Objective ring ACR for objective sleeve cylindrical long

CAN Distributor for Motorized Objectives and Filter Wheels for up to 3 objectives autocorr and 1-2 filter wheels

Autoimmersion module for selected water immersion objectives on Axio Observer 7 controllable by ZEN blue software and TFT consisting of: Pump module with CAN connection cable Tube holder for nosepiece Transparent objective ring with feed pipette holder 3x Feed pipette with tube Adapter for feed pipette Water absorption rings (foam rings), 1x small, 1x large Liquid reservoir (threaded bottle) Syringe set with Luer lock connector Spare tubing 550 mm

LD condenser 0.55 mot.; AI Sample Finder - with motor operated aperture diaphragm and 7 turret disc positions for H, Ph1, Ph2, Ph3, 2x DIC as well as additional position for autofocus and coherence contrast - LED array for contrast enhancement in the overview image - Camera module for overview image - Deflecting mirror for LD condenser 0.55 mot. AI Sample Finder - Calibration plate 160x110 AI Sample Finder - WD=26 mm - for objective magnifications 4,0x-100x

Stage Controller XY STEP SMC 2009 Control unit for Maerzhaeuser XY-Stages with stepper motors. - bidirectional communication to microscopes with CAN - position display on microscopes with TFT display and option for objective depended travel speed - 2 CAN interfaces - alternative connection through USB or RS232 - power supply 100...240V AC/50...60Hz - incl. USB-cable, CAN-cable and plug-in power unit with country specific power cord

Joystick XY; CAN (D) for mechanical stages mot; CAN, scanning stages; CAN and stage controller XY STEP SMC 2009 - 2 speed ranges selectable - direct cable connection to CAN stage or to stage controller XY STEP SMC 2009

Extension cable for stage control 1.6 m (D) for usage with joystick, trackball or coaxial drive. Recommended for adaptation of cable length when a docking station is used.

Gas Mixer System CO2, ibidi® (D) For CO2 control and humidification - Includes controller, humidifying column and humidity sensor to be mounted at Glass Lid CO2/O2 heated/unheated - Includes 2 x 5m gas tubing (PUN-H) - The Gas Mixer System requires pressured air (1 bar ) Software: - ibidi® IncuControl - CO2 regulation up to 15% - CO2: resolution 0.1%, precision +/- 0.35% at 5% CO2 - Humidity regulation: 20-99%, resolution 1%, precision +/- 5% - no manual calibration required Connections: - Input channel for humidity sensor for feedback regulation - USB- and CAN connection - External power supply 100-240V / 50-60Hz / max. 1.4 A; Output: 24V DC / max. A, 120W; with country specific plug - Dimensions controller: 170 mm x 230 mm x 90mm (W x D x H) - Dimensions humidifying column: 70 mm x 110 mm x 300 mm (W x D x H) ibidi® is a registered trademark of the ibidi GmbH

Temperature Controller, ibidi® (D) - For temperature control of up to 4 electrically heated components: Glass Lid CO2/O2 heated, ibidi® Heated Plate K with 3 inserts, ibidi® Heating Unit XL (PeCon) Objective heater (PeCon) -includes Temperature Adjustment Set: -Type K thermocouple and 3 dishes for temperature adjustment (µ-Dish 35 and 50 mm, µ-Slide 8 well) Connections: - Input channel for thermocouple to control sample temperature - USB and CAN connection Software: - ibidi® IncuControl Resolution. 0.1°C Precision: +/- 0.5°C Temperature Control Range: Channel 1 (Heated Lid): Ambient temperature (min. 18°C) to +46°C Channel 2 (Heated Plate): Ambient temperature (min. 18°C) to +45°C - incl. power supply 100-240V / 50 - 60Hz; Output: max. 2A; 24V DC / max. 6.67A 160W - Dimension: 230 mm x 170 mm x 90 mm (W x D x H) ibidi® is a registered trademark of the ibidi GmbH

Glass Lid CO2/O2 heated, ibidi® (D) To be used with Temperature Controller, ibidi® - Suitable for ibidi® Heated Plate K with 3 inserts and ibidi® Mounting frame K for multiwell plates - Temperature range: ambient to 45°C - Compatible with DIC contrast - 4 openings for perfusion tubing (width 3 mm, height 7 mm) - Opening for humidity sensor (part of ibidi® Gas Mixer System) - Luer Lock Connector for heated gas tubing (ibidi® Gas Mixer System) - The Gasmixer System requires pressured air (1 bar) - Observation area: 142 mm x 70 mm - Outer dimensions: 160 mm x 110 mm x 19 mm (W x D x H) ibidi® is a registered trademark of the ibidi GmbH

Heated plate K with 3 inserts, ibidi® (D) To be used with Temperature Controller, ibidi® - Suitable for mechanical and scanning stages with opening 160 x 110 mm - Including inserts for: - Slides and Chamber Slides (62...78 mm length to 26.5 mm width or 57...70 mm length to 27.5 mm width) - 35 mm Petri dishes (32 - 44 mm diameter, 9 - 14 mm height) - 60 mm Petri dishes (48 - 68 mm diameter, 9 - 14 mm height) - Temperature range: ambient to 45°C - Outer dimensions: 160 mm x 130 mm x 12 mm (W x D x H) ibidi® is a registered trademark of the ibidi GmbH

Signal distribution box Rev.3 for Trigger-Board

Microscopy Workstation Premium (HP Z8 G5) (O) HP Z8 Fury G5 Workstation - Chipset: Intel W790 - Memory Expansion Slots: 16 x DDR5, RDIMM, ECC, 4800 MHz Modules - Memory: max. 1024 GB RAM - PCI Express Connectors: 2 x PCI Express Generation5 x16 1 x PCI Express Generation 5 x4 2 x PCI Express Generation 4 x16 2 x PCI Express Generation 4 x4 1 x PCI Express Generation 3 x8 - SSD: 1 x 2 TB M.2 NVMe - Hard Drives: 2 x 12 TB SATA 7200 rpm (configured as 10 TB RAID 1hard drive) - Network Controller: 2 x GbE LAN interface (RJ 45) - USB: 2 x USB 3.2 Gen2x2 type C (front), 8 x USB 3.1 Gen 1 type A (2 x front, 6 x rear) - Certifications <(>&<)> Declarations: CE, CSA, UL, CCC, KCC - Power Supply: 100 - 240 V / 50 - 60 Hz / 1450 W with country specific plug - Chassis Dimensions: (H x W x D): 445 x 216 x 555 mm - USB Mouse, Recovery kit

System Configuration 2 for HP Z8 G5 (O) Installation of Windows 10 IoT Enterprise LTSC 2021 x64 incl. Windows 10 License - with ZEN software installation and system integration of respective system - for Celldiscoverer 7, LSM 900, LSM 980, Elyra 7, Lattice SIM, Axioscan 7, Lattice Lightsheet - only for 410203-9914-000 Microscopy Workstation Premium HP Z8 Fury G5

Processor Intel Xeon W7-3455 (HP Z8 G5) (O) High performance processor configuration - Intel Xeon W7-3455 (2.5 GHz, 67.5 MB cache, 24 cores) - only for 410203-9914-000 Microscopy Workstation Premium HP Z8 Fury G5

Memory 32 GB (1x32) DDR5 4800 MHz ECC reg RAM (HP Z8 G5) (O) consisting of 1 x 32 GB module - DDR5 4800 MHz ECC registered DIMM - can be extended to 64 GB (2 x 410303-3206-000), 128 GB (4 x 410303- 3206-000) or 256 GB (8 x 410303-3206-000) - only for 410203-9914-000 Microscopy Workstation Premium HP Z8 Fury G5

Graphics Card NVIDIA RTX A5000 24 GB DP (O) Professional graphics card for Microscopy Workstation - 24 GB GDDR6 memory - PCI Express 4.0 Interface x16 - Hardware acceleration of DirectX 12.07, OpenGL 4.68 and CUDA - Support for up to four displays - Display outputs: 4 x DisplayPort 1.4a (DP) - DisplayPort support for 4 x 32" Display (3840 x 2160 Pixel)

Language Pack English US (O) - Keyboard with English (US) layout

Microscopy Camera Axiocam 820 mono High performance microscopy monochrome camera incl. driver software 64bit, Interface card USB 3.1 Gen2 PCIe x4, dual USB 3.0 cable 3 m Sensor Type: SONY IMX541 CMOS, monochrome, Global Shutter "Backside Illuminated" Number of Pixels: 4512 (H) x 4512 (V) = 20.3 Mega Pixels Pixel Size: 2.74 µm x 2.74 µm Chip Size: 12.4 mm x 12.4 mm, (square field of view), equivalent to 1.1" (17,4 mm diagonal) Spectral Range: With protection glass. 350 nm to 1000 nm Peak QE: 86% @ 520 nm (typ.), "backside illuminated" design Full Well Capacity: Approx. 10,000 e at gain 1x (typ.) Adjustable Signal Amplification: 1x, 2x, 4x, 8x, 16x Selectable Resolution: H x V Binning Factor (digital) 4512 x 4512 1x1 basic pixelcount 2256 x 2256 2x2 1504 x 1504 3x3 1128 x 1128 4x4 902 x 902 5x5 (at 2x2 combined analog and digital binning for further increase of sensitivity) H x V Subsampling Factor(full field of view) 4512 x 4512 1x1 2256 x 2256 2x2 Live frame rates (depending on hardware and software configuration): Exp. Time 1ms H x V Frame Rate 4512 x 4512 >20 fps (High Res mode, high CPU load) 2256 x 2256 >30 fps (default: Fast Mode, reduced CPU load) Frame rates for time series recording (1 ms exposure, further depending on Sensor ROI, hardware and software configuration) H x V (ROI) FrameRate (fps) 4512 x 4512 28 2256 x 2256 75 (2x2 subsampling; full field of view) 2248 x 1504 82 1920 x 1080 110 1024 x 1024 115 512x x 512 207 1920 x 256 346 1920 x 128 447 Read Noise Full Well Capacity Dynamic Range (at gain) 2.3 e (1x) 10,000 e 1:4440 1.8 e (2x) 5,500 e 1:2720 1.6 e (4x) 2,500 e 1:1540 1.5 e (8x) 1,250 e 1: 850 1.3 e (16x) 625 e 1: 470 HDR Mode: 1,6 e 40,000 e 1:25,000 Software Supported Camera Operation: Exposure time adjustment with continuous, automatic or manual mode, Adjustable over or under-exposure mode, Optional measurement on defined target area (spot-meter), Image sharpening, Dark current compensation, Single pixel noise correction Readout of Sensor Sub Regions ("ROI"): Adjustable Digitization: 8, 12, 14 bit / pixel (8 out of 14 bit or compression mode) Dark Current: <(><<)> 0.1 e/p/s @ 25 °C sensor temperature Cooling: Stabilized temperature to 25 °C (mounted on microscope, free air flow, ambient temp. 22 °C) Exposure Time Range: 0.1 ms to 60 s Hardware Trigger: Trigger-In for exposure timecontrol Trigger-Out for exposure time Readout time and trigger ready status (further accessories required) Status-LED for Camera: Color coded operation status, dimmable Camera Interface: Dual USB 3.0 SuperSpeed, 2 x 5 Gbit/s for data and power supply (optional single USB 3.0 at lower framerates and without cooling) OpticalInterface: C-Mount Thread Depth for Objectives: Max. 5 mm Size / Weight: Approx. 10.8 cm x 7.8 cm x 6.1 cm (4.2" x 3.1" x 2.4") / 620 g Housing: Blue anodized aluminum, with cooling fins on top 1/4" photo thread for tripod mount Registration: CE Power Supply: Provided by Dual USB 3.0, 5V, max. 7 W power consumption Environmental Conditions: 5 °C to 35 °C, max. 80% relative air humidity, not condensing, free air circulation required Supported Operating Systems: for ZEN: Windows 10 x64 Enterprise Supported Application Software: ZEN 3.6 (blue edition) or higher, ZEN 3.5 core or higher The required application software is provided via ZEISS Microscopy Installer or ZEISS Download Center.

Trigger Cable Set Axiocam 5xx/7xx/8xx (D) for direct shutter synchronization using trigger board with signal distribution box, Colibri or HXP 120 V

ZEN 3.9 Image acquisition and processing under Windows 10 x64 User interface configurable, control of the ZEISS microscope systems and components, extensive acquisition and analysis, CZI image format The following functions are included: - arivis cloud advanced - Basic 3D viewing - Colocalization - Connect basic - Direct processing - Manual EDF - Measurement - Panorama - Spectral unmixing - ZEN Data Storage Client - Acquisition Base (time series and multichannel)

ZEN Toolkit Motorized Acquisition Acquire images and control motorized components in multi-dimensional experiments. This package allows to acquire tiles, multi-position and z-stack images using motorized components. These can be combined with the included autofocus and direct processing. z-stack and enhanced depth of focus functionality: - definition of the Z-stack in "Start/Stop" and "Center" mode - size of the configurable Z-stack and number of possible Z-planes only limited by the travel range of the system and the minimum increment - "Optimal" button to automatically set the correct increment to satisfy the Nyquist criterion - integrated Z-drive backlash compensation for maximum precision and reproducibility - extraction of the sharp details from individual images at various focus positions and combination into an image with high depth of field Tiles <(>&<)> Positions - automatic scanning of predefined sample areas - acquisition of images according to a position list - support of focus correction maps, stitching and shading correction - compatible with software autofocus Software Autofocus: - determination of the optimal focus position in the sample - the autofocus works with transmitted, reflected and fluorescence light - definition of an autofocus region in the camera image

ZEN Toolkit 2D Create flexible automatic measurement programs for the analysis of your 2D images. Set up an automatic measurement program for analyzing your data sets (e.g., time series, tile images and multiscene images) in just a few steps. A user-friendly wizard guides you through all the necessary settings, starting with the definition of the classes and the segmentation of objects, to the selection of measurement parameters. Create simple measurement programs consisting of a single class or set up powerful measurement programs with multiple classes, which you can also arrange hierarchically (objects within objects) or use the predefined zone-of-influence* measurement program if you want to detect objects in the periphery of other objects. Flexibly choose between different classical and AI-based segmentation methods for each class. You can improve your results using AI models (semantic or instance segmentation) especially for datasets which are particularly difficult to segment. This toolkit is "AI-ready", which means you can use any trained AI model in the analysis program without requiring another license. Simply load a model previously trained with the ZEN Toolkit AI in ZEN or ZEN core into the Image Analysis Wizard, or use DNNs trained on arivis Cloud. Subsequently, you can further refine the segmentation results, e.g., by using algorithms for object separation or by applying region filters, if necessary. To quantify the detected objects, a variety of measurement features are available, such as different geometrical (size, shape,...) or intensity features as well as user-defined measurement parameters*. After the analysis, the segmented images are displayed along with result tables and diagrams (e.g., scatterplot or heatmaps). Tables, diagrams, and images are linked so that you can interactively explore your results. You can also use the created image analysis programs for batch processing of multiple data sets. In addition, this toolkit also includes the Bio App Cell Counting*. (*not available in ZEN core) For preprocessing of your image data, the toolkit additionally provides a number of special image processing functions belonging to the Edges (e.g. Highpass, Sobel, Laplace,…), Arithmetic (e.g. Addition, Multiply Constant,…), Morphology (e.g. Erode, Dilate, TopHat,…), Segmentation (e.g. Canny, Marr…) and Binary (e.g. Fill Holes, And, Or,…) Operations groups. Requirements: - The use of AI semantic segmentation requires an installation of Zeiss Python - The use of AI instance segmentation requires an installation of Docker Desktop For the use of AI models the recommended hardware configuration is 8GB GPU and 64GB RAM. Only the CPU and Nvidia GPUs are supported.

ZEN Toolkit Deconvolution Sharpen your images with mathematically exact deconvolution and make previously unknown structures visible by increasing your image contrast and resolution. Execute the underlying iterative 3D DCV algorithms by leveraging multi-GPU in just minutes. You have the choice between preset parameters and free parameter selection in expert mode.

ZEN Toolkit Molecular Quantification Includes functions for the for investigation of diverse dynamic and molecular interaction phenomena of diverse specimens Physiology Tool for the analysis of physiological time series data, with Ratiometric measurements, including mean ROI measurement. Includes specialised charting and viewing functions FRAP Efficiency Analysis Analysis of FRAP/FLIP or similar time series acquisitions with bleach events to determine the half time of recovery / decrease of fluorescent signals using mono or bi-exponential fit algorithms including options for background correction, correction of imaging induced photo bleaching and the possibility to evaluate grouped ROIs. FRET Analysis of FRET data which have been created with either "Sensitized Emission" or "Acceptor Photobleaching" approaches. Includes a special FRET View with calculation of control parameters, a color-coded display of the resulting images as well as the intensity changes of selected image regions. The most common methods are supported: Gordon, Xia, Youvan. Automated Photomanipulation For LSM 900/980 and Celldiscoverer 7 with LSM 900 For automated photoactivation and bleaching at multiple positions. The ROIs for photomanipulation are automatically determined and applied in the experiment. Allows the automated acquisition of an image at every position, the identification of the ROIs via image analysis followed by the photomanipulation experiment. The photomanipulation is executed either a tall tile regions per time point (photoactivation) or as full time series per tile region (bleaching).

Dust cover for Axio Observer / Axiovert 200/200M (L600xB420xH620 mm)

PC Interface Microscope and Trigger for Celldiscoverer 7 (D) Trigger board for TTL trigger output and analysis. with precise trigger control of Celldiscoverer 7, LSM and up to two triggerable cameras.