Microscope Axio Zoom.V16

Cold-light source CL 9000 LED CAN (D) - up to 900 lm light flux at 9 mm fiber output dia., ca. 6500K color temperature - min. 50,000 operating hours until intensity drop to 70% - control of intensity and 6 memory cells by rotary push knob and LCD - 10 mm collet chuck with sensor for 1500 light guides - filter slider for 2 mounted filters 35 x 26 x 4 mm and with free aperture - max. 80W wide-range supply for flicker free light, silent fan - 2.5 mm jack bush for on/off via optional foot button S - BNC socket for trigger input - 2 CAN RJ45 sockets for light control by EMS 3 - CAN RJ45 cable 1 m, country-specific power cable - Dimensions D241xW192xH118 mm, weight 3.1 kg

Slit-ring illuminator 1500 ECO, d=66 mm, 9/1000 mm (D)

Halogen Light Filter CL LED (D)

Day Light Filter CL LED (D)

Mounting frame and specimen holder four samples (D) The mounting frame with integrated sample holder is accessory for technical cleanliness and applicable for four filter membranes with diameter 47 mm.

Camera Adapter 60N-C 2/3" 0.63x; rotating +/- 2° for fine adjustment of camera rotation. - adjustment range: +/- 2° - maximum weight load: 2 kg

Stage micrometer for reflected light 5 + 100/100y d=0mm - 5 lines with 1mm intervals in y-direction - 100 lines with 1/100mm intervals in y-direction

Eyepiece PL 10x/23 Br. foc.

Eyepiece eyecup

Microscope body Axio Zoom.V16 - apochromatic motor zoom 16:1 (0.7x ... 11.2x), with more than twice the aperture of stereo zoom, - motorized aperture diaphragm with scroll wheel and 3 variable control modes: 1. "Brightness" for maximum of image brightness with 10x eyepieces or maximum of resolution with 16x or 25x eyepieces, 2. "Camera" for constant brightness and resolution along zoom range fitted to high-resolution image sensor, 3. "Eyepiece" for large depth of field in lower zoom range and resolution fitted to 10x eyepieces; - objective support M55x1.5, removeable for nosepiece assembling, - position sensors for objective nosepiece Z, - 76mm support dia. Fitting 435402 carrier S or 435380-9000 nosepiece Z, - additionally required HIP for supply and operation (in Brightness mode only) or EMS 3 + SYCOP 3 for mode control and USB interface

Controller EMS 3 includes 150 W wide-range power supply, on/off button, on rear side mains switch and interfaces: - 5x CAN-RJ45 for CL 9000 LED CAN, SteREO or Axio Zoom components, - 1x Power/CAN, cable 435403-9030 to focus motor 3 or 435610-5003 to motor 2 additionally required, - 1x SYCOP 3, 2x KL 2500 LCD, 2x footswitch, - 2x CAN-HD15 for Axio accessories (motor stage, light sources), - 1x USB to work station, 1x RS232 (D-Sub DE9), incl. USB cable 2m and country-specific power cable

System Control Panel SYCOP 3 5.7" color touch screen with LED back illumination, joystick, 2 scroll wheels and 6 freely configurable keys; incl. 2 m cable to EMS 3

Binocular phototube Z 15° manual switching 100 vis : 100 doc

Objective PlanNeoFluar Z 1.0x/0.25 FWD 56mm with resolution of 100 ... 740 LP/mm or smallest detail 5.0 ... 0.7 µm; 133mm parfocal length; M62x0.75 thread at front

Mount S with diam. 76 mm support

Motor focus 3 with column S 490mm - Lifting range 340 mm, load capacity 18 kg - For stand bases 300/450

Stand base 450 WxDxH 449x346x30mm stand base with 3x M8 threaded holes, insert plate 450 and b/w plate D=120mm; compatible with column S 490/800 mm or T column 350mm and with transmitted light unit 450 or 450 mot or VisiLED transmitted light; stage d=84mm needs adapter 84/120 (435430-9010) additionally

Cable EMS3/CMD - focus motor 3

Measuring stage S 150x100 mot; CAN (D) - 3-plates mechanical stage (495...530)x(280...390)x(28...95)mm for max. load 3kg, - specimen bearing 340x207mm and 237x157mm support for insert plate S, - stepper motors, encoders and integrated controller, - variable speed up to 17mm/s, - speed up to 100mm/s in automatic find action, - reproducibility +/- 1µm - absolute accuracy +/- 2µm - interface d=120mm, BNC trigger and CAN-HD15, incl. CAN-HD15 cable, - useable with transillumination

Adapter frame S 160x116 epi-illumination (D) holding frames and plates 160x116 of Axio Imager are adapted to stages S 150x100, using together with transillumination top 450 mot (435500-9000) is not allowed

Polarization filter set S, d=66 mm (D) consisting of fixed analyzer and rotary, annular polarizer

Joystick XY; CAN (D) for mechanical stages mot; CAN, scanning stages; CAN and stage controller XY STEP SMC 2009 - 2 speed ranges selectable - direct cable connection to CAN stage or to stage controller XY STEP SMC 2009

Microscopy Workstation Premium (HP Z8 G5) (O) HP Z8 Fury G5 Workstation - Chipset: Intel W790 - Memory Expansion Slots: 16 x DDR5, RDIMM, ECC, 4800 MHz Modules - Memory: max. 1024 GB RAM - PCI Express Connectors: 2 x PCI Express Generation5 x16 1 x PCI Express Generation 5 x4 2 x PCI Express Generation 4 x16 2 x PCI Express Generation 4 x4 1 x PCI Express Generation 3 x8 - SSD: 1 x 2 TB M.2 NVMe - Hard Drives: 2 x 12 TB SATA 7200 rpm (configured as 10 TB RAID 1hard drive) - Network Controller: 2 x GbE LAN interface (RJ 45) - USB: 2 x USB 3.2 Gen2x2 type C (front), 8 x USB 3.1 Gen 1 type A (2 x front, 6 x rear) - Certifications <(>&<)> Declarations: CE, CSA, UL, CCC, KCC - Power Supply: 100 - 240 V / 50 - 60 Hz / 1450 W with country specific plug - Chassis Dimensions: (H x W x D): 445 x 216 x 555 mm - USB Mouse, Recovery kit

Processor Intel Xeon W5-3425 (HP Z8 G5) (O) Default processor configuration - Intel Xeon W5-3425 (3.2 GHz, 30 MB cache, 12 cores) - only for 410203-9914-000 Microscopy Workstation Premium HP Z8 Fury G5

Memory 32 GB (1x32) DDR5 4800 MHz ECC reg RAM (HP Z8 G5) (O) consisting of 1 x 32 GB module - DDR5 4800 MHz ECC registered DIMM - can be extended to 64 GB (2 x 410303-3206-000), 128 GB (4 x 410303- 3206-000) or 256 GB (8 x 410303-3206-000) - only for 410203-9914-000 Microscopy Workstation Premium HP Z8 Fury G5

Graphics Card NVIDIA RTX A2000 12 GB (O) Professional graphics card for Microscopy Workstation - 12 GB GDDR6 memory - PCI Express 4.0 Interface x16 - Hardware acceleration of DirectX 12.07, OpenGL 4.68 and CUDA - Support for up to four displays - Display outputs: 4 x MiniDisplayPort 1.4 (mDP) - DisplayPort support for 4 x 32" Display (3840 x 2160 Pixel)

System Configuration 2 for HP Z8 G5 (O) Installation of Windows 10 IoT Enterprise LTSC 2021 x64 incl. Windows 10 License - with ZEN software installation and system integration of respective system - for Celldiscoverer 7, LSM 900, LSM 980, Elyra 7, Lattice SIM, Axioscan 7, Lattice Lightsheet - only for 410203-9914-000 Microscopy Workstation Premium HP Z8 Fury G5

Language Pack English US (O) - Keyboard with English (US) layout

Monitor TFT 24" HP Z24n G3 (61 cm) (O) Visible diagonal: 61 cm, 16:10 Maximum resolution: 1920 x 1200 pixel @ 60 Hz Brightness: 350 nits Contrast: 1000:1 Viewing angle: horizontal 178 degree, vertical 178 degree Video input: DisplayPort 1.2, HDMI 1.4 Data connectors: 1 x USB Type B (upstream), 4 x SuperSpeed USB Type-A 5Gbps Power consumption: Maximum 63 W, Typical 28 W, Standby 0.5 W Dimensions incl. stand (H x W x D): 52.1 cm x 53.2 cm x 19.5 cm Weight incl. stand: 4.9 kg Ergonomic features:Tilt: -5°to +20°, Swivel: -45° to +45°, Height adjustable 15 cm, Pivot rotation: -90° to +90° Certification and compliance: CE, CB, GS, TÜV, FCC, TCO Operating voltage: 100-240 V, country-specific power cable

Microscopy Camera Axiocam 705 pol R2 (D) High Performance microscopy camera incl. driver software 64 bit USB 3.0 PCIe x1 interface, USB 3.0 cable 3 m and protection glass (wideband coated) Sensor Type: SONY IMX250 MZ Exmor CMOS, SONY Pregius Global Shutter Monochrome with 2x2 pixel specific polarization filter mask (90°, 45°, 135°, 0°) Number of Pixels: 2464 (H) x 2056 (V) = 5.07 Mega pixel Pixel Size: 3.45 µm x 3.45 µm Chip Size: 8.5 mm x 7.1 mm, equivalent to 2/3" (diagonal 11.1 mm) Spectral Range: With protection glass approx. 350 nm to 1000 nm Full Well Capacity: Approx. 11,000 e at gain 1x (typ.) Adjustable Signal Amplification: 1x, 2x, 4x, 8x, 16x Selectable Resolution: H x V Binning Factor (1x1) 2464 x 2056 1x1 Basic Resolution 816 x 684 3x3 480 x 410 5x5 (at 2x2 combined analog and digital binning for further increase of sensitivity) Live Frame Rates: (1 ms exposure, default setting, simplified color coding) H x V Frame Rate (fps) 2464 x 2056 25 (1ms exposure time, ROI 1920 x 1080, standard color coding) H x V Frame Rate (fps) 1920 x 1080 5,5 Read Noise Full Well Capacity Dynamic Range (at gain) 2.20 e (1x) 11,000 e 1:5000 1.74 e (2x) 5,000 e 1:3100 1.48 e (4x) 2,700 e 1:1800 1.29 e (8x) 1,300 e 1:1300 1.15 e (16x) 690 e 1: 600 Software Supported Camera Operation: Exposure time adjustment with continuous, automatic or manual mode, adjustable over or under-exposure mode, optional measurement on defined target area (spot- meter) Readout of Sensor Sub Regions ("ROI"): Adjustable Digitization: 8, 12, 14 bit / pixel (8 out of 14 bit or compression mode) Dark Current: <0.5 e/p/s @ 18 °C sensor temperature Cooling: Stabilized temperature to 18 °C (for ambient temperatures from 18 °C to 30 °C) Exposure Time Range: 0.1ms to 60 s Hardware Trigger: Trigger-In for exposure time control, Trigger-Out for exposure time, readout time and trigger ready status (further accessories required) Status-LED for Camera: Color coded operation status, dimmable Camera Interface: USB 3.0 (Optional operation withUSB 2.0 at reduced frame rate) Optical Interface: C-Mount Thread Depth for Objectives: max. 5 mm Size / Weight: Approx. 10.8 cm x 7.8 cm x 5,1 cm (2.3"x 3.2" x 2,0") / 580 g Housing: blue anodized aluminum, with cooling fins on top, 1/4" photo thread for tripod mount Registration: CE Power Supply: Provided by USB 3.0 und USB 2.0, max. 7 W consumption Environmental Conditions: 5 °C to 35 °C, max. 80% relative air humidity, not condensing, free air circulation required Supported Operating Systems: for ZEN: Windows 10 x64 Supported Application Software: ZEN 3.6 (blue edition, Hotfix 3.6.6) or higher, ZEN core 3.5 (Hotfix 3.5.4) or higher The required application software is available via the ZEISS Download Center.

ZEN core 3.9 Software suite for microscope image acquisition, processing, analysis, and data connectivity. - Configurable graphical user interface including workbench concept for customized routine jobs and workflows - Full integration of the ZEISS microscopes (incl. Visioner 1) that can be configured in MicroToolbox, Axiocam cameras and additional components - Interactive and automatic control of the individual motorized microscope components and transfer of information from encoded components into the software - Image acquisition with b/w, rgb, high-resolution and high-sensitivity cameras - HDR, Panorama, and manual Extended Depth of Focus (EDF) acquisitions - Basic image acquisition on electron microscopes - Image processing: standard operations for image optimization (contrast, brightness, gamma, colors, smoothing, sharpening, geometric corrections...) - Interactive/dimensional measurements, including online measurements on the live image - Input Form Designer with automated customer data import - Report creation and modification of Microsoft Word report templates using ZEN Word add-in - Data Archive for managing and maintaining documents: searching, filtering, tagging, viewing, re-opening, structuring, exporting, importing (any file format via drag n drop) - User Management for multi-user environment incl. full integration into the Windows multi-user functionality - Connectivity to ZEN Data Storage image database - Contains ZEN Connect basic functionality, 2D CAD overlay, topography viewer for 2.5D visualization of EDF Images incl. profile line measurement, QUAL Data Export - arivis Cloud connector enables the execution of customized solutions by integrating modules from the arivis Cloud platform Supported operating systems: Windows 10 (64-Bit)

ZEN Module Technical Cleanliness Analysis Software module for evaluation of particulate contamination on prepared specimen. The following standards for component cleanliness, oil cleanliness, air cleanliness and cleanliness of medical products are supported: VDA 19.1, ISO 16232, ISO 4406, ISO 4407, NAS 1638, SAE AS 4059,VDA 19.2, VDI 2083 (Sheet 21). The scope of delivery includes preconfigured automated workflows for technical cleanliness analysis and rating of single or multiple specimen for the user roles Supervisor and Operator. Provided job templates (I-V): Workflow Creator Wizard for application guided selection and auto-generation of the following job templates for technical cleanliness analysis: - The workflows I - III are operable with acquired or loaded images. - The correlative workflows IV - V require image acquisition. (I) automated workflow for acquisition of multichannel images with different polarization directions in combination with Axiocam 705 pol mainly for the evaluation of component cleanliness and the rating of technical cleanliness of medical products. The use of latest camera sensor technology with on-chip polarization enables a workflow with one single specimen acquisition instead of double image acquisition of the complete specimen area with different contrast methods. This leads to a significant time saving. (II) automated workflow for acquisition of single channel images mainly for oil analysis. (III) automated workflow for component cleanliness testing with AI-based object classification. Contains trainable, pre-trained models for AI- based analysis of multichannel images with different polarization directions. Training of the delivered machine learning models requires ZEN Toolkit AI . (IV) light microscope workflow for correlative technical cleanliness analysis (component and oil cleanliness) with automated and/or manual particle selection for subsequent SEM/EDS analysis in Free Mode. Requires in addition ZEN Toolkit Connect. (V) light microscope workflow for correlative component cleanliness analysis with AI-based object classification. Contains trainable, pre- trained models for AI-based analysis of multichannel images with different polarization directions. Supports automated and/or manual particle selection for subsequent SEM/EDS analysis in Free Mode. Training of the delivered machine learning models requires ZEN Toolkit AI. Requires in addition ZEN Toolkit Connect. The job templates cover image acquisition, image analysis and image processing, result presentation in interactive views for fast inspection and revision of detected particles, reports with results of characteristic values and standard specific methods per tested specimen. Automated storage of generated results and inspection views in the data archive. Inspection and export of archived data. (VI) post analysis workflow from archived data for calculation and interpretation of results to monitor air particles using particle traps supporting VDA 19.2 The job templates cover image acquisition, image analysis and image processing, result presentation in interactive views for fast inspection and revision of detected particles, reports with results of characteristic values and standard specific methods per tested specimen. Automated storage of generated results and inspection views in the data archive. Inspection and export of archived data.

ZEN Toolkit Motorized Acquisition Acquire images and control motorized components in multi-dimensional experiments. This package allows to acquire tiles, multi-position and z-stack images using motorized components. These can be combined with the included autofocus and direct processing. z-stack and enhanced depth of focus functionality: - definition of the Z-stack in "Start/Stop" and "Center" mode - size of the configurable Z-stack and number of possible Z-planes only limited by the travel range of the system and the minimum increment - "Optimal" button to automatically set the correct increment to satisfy the Nyquist criterion - integrated Z-drive backlash compensation for maximum precision and reproducibility - extraction of the sharp details from individual images at various focus positions and combination into an image with high depth of field Tiles <(>&<)> Positions - automatic scanning of predefined sample areas - acquisition of images according to a position list - support of focus correction maps, stitching and shading correction - compatible with software autofocus Software Autofocus: - determination of the optimal focus position in the sample - the autofocus works with transmitted, reflected and fluorescence light - definition of an autofocus region in the camera image

Dust protection Set M consisting of dust cover (L650xW200xH570 mm), eyepiece cover and protection cap for luminous-field diaphragm optics