Objective i LCI Plan-Neofluar 63x/1.3 Imm Corr DIC M27

Objective i LCI Plan-Neofluar 63x/1.3 Imm Corr DIC M27

Item no.: 420882-9970-720
(no longer available)
Description

Objective i LCI Plan-Neofluar 63x/1.3 Imm Corr DIC M27 with insulation ring for optimized temperature at the specimen, for water, silicone oil or glycerine immersion (CG=0.15-0.19mm) (FWD=0.17mm at CG=0.17mm) incl. Immersol W, bottle 20 ml and Cover glasses, high performance, CG=0.17mm, box with 100 pc.

Full Description

With insulation ring for optimized temperature at the specimen.

The rating of the option Definite Focus.2 is valid for use with glycerine immersion.

Magnification 63x
Numerical Aperture 1.3
Free Working Distance [mm] 0.17 at cover glass 0.17
Coverglass Thickness [mm] 0.15 - 0.19
Thread Type M27x0.75
Immersion Water, W 2010, Silicone oil and Glycerine
Field of View [mm] 20
Parfocal Length [mm] 45.06
Long Distance
Correction Ring Yes
Iris
Optical System Infinity Color Corrected System (ICS)
Color Correction semi-apochromatic
Biomedical Applications
Fluorescence Yes
- Multichannel
- Ultraviolet Transmission
- Infra Red Transmission
BrightField Yes
DIC [Differential Interference Contrast]
High Contrast DIC Yes
PlasDIC
Phase Contrast
VAREL Contrast
Hoffman Modulation Contrast
Polarization Contrast
Materials (Reflected Light) Applications
BrightField
BrightField/DarkField
Reflected Light DIC
High Contrast DIC Yes
DIC with circular polarized light
Total Interference Contrast
Polarization Contrast
Options
Definite Focus.2
Confocal Microscopy Yes
- Ultra Violet
- VIS (visible light)
- NLO-IR / 2 Photon
Total Internal Reflection Fluorescence
Apotome Yes
Microdissection
  • Objective heater 33/38 S1 (D)
    Objective heater 33/38 S1 (D)
    Item no.: 411860-9067-000
  • Objective ring ACR for objective sleeve conical long
    Objective ring ACR for objective sleeve conical long
    Item no.: 424510-0000-000
  • Adapter 30mm with optics and DIC slider mount
    Adapter 30mm with optics and DIC slider mount
    Item no.: 424516-9041-000
  • DIC slider LCI PN 63x/1.30 III
    DIC slider LCI PN 63x/1.30 III
    Item no.: 426959-0000-000
  • Immersion medium Immersol W 2010, oiler 20 ml
    Immersion medium Immersol W 2010, oiler 20 ml
    Item no.: 444969-0000-000
  • Immersion medium Immersol Sil 406, Oiler 20 ml
    Immersion medium Immersol Sil 406, Oiler 20 ml
    Item no.: 444971-9000-000
  • Immersion medium Immersol G, oiler 20 ml
    Immersion medium Immersol G, oiler 20 ml
    Item no.: 462959-9901-000
  • Box of lens cleaning paper (300 sheets)
    Box of lens cleaning paper (300 sheets)
    Item no.: 462975-0000-000
  • Cover glasses, high performance, D=0.17mm, box with 1000 pc.
    Cover glasses, high performance, D=0.17mm, box with 1000 pc.
    Item no.: 474030-9000-000
Mechanical Dimensions
Transmittance curve
LCI Plan-Neofluar

These objectives have been specifically designed for Life Cell Imaging techniques and are designed for the temperature interval between 23° and 37°C. The 25x/0.8 can be used with the immersion mediums water, glycerine and oil, while the 63x/1.3 can be used with water and glycerine. A single correction collar permits simple and convenient compensation for spherical aberration caused, for example, by different cover slip thicknesses, temperature drift or immersion media with a different refractive index, adjustable between water and glycerine. For the examination of living objects, water is particularly suitable as immersion medium. Furthermore, cleaning is extremely easy. For critical fluorescence examinations, cleaned glycerol is the ideal immersion medium, as it has very low autofluorescence. Therefore, glycerol and water should be preferred for the microscopy of living objects, since these are found in a medium with a similar refractive index. The "Refractive Index Mismatch" indicates the deviation of the actually available refractive index from the one that the objective has been calculated for. This deviation too can be compensated for by means of the correction collar. Used with inverted microscopes, multi-immersion objectives are ideal for the limited compensation of non-standard glass bottom thicknesses (D < 0,17) or the popular use of water as immersion medium with a slightly lower numerical aperture than oil-immersion objectives.
Special emphasis has been placed on good accessibility of the optical axis with micromanipulators.

Objective i LCI Plan-Neofluar 63x/1.3 Imm Corr DIC M27 with insulation ring for optimized temperature at the specimen, for water, silicone oil or glycerine immersion (CG=0.15-0.19mm) (FWD=0.17mm at CG=0.17mm) incl. Immersol W, bottle 20 ml and Cover glasses, high performance, CG=0.17mm, box with 100 pc.