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Fluorescence Dye and Filter Da...

Overview Dyes

Note: This overview shows dyes with the assigned filter sets. Please feel free to point out any important missing fluorochromes, or fluorochromes no longer used. Please be aware that shown maximum values of excitation and emission do not necessarily apply in every instance. For this reason recommended filter sets may not be optimal in every case. If the operator uses another salt of the compound, or the pH-value is different, excitation and emission values may vary greatly. In such cases we are happy to provide assistance.

Sample Fluorescent Dyes   Excitation   Emission  
1,8-ANS (1-Anilinonaphthalene-8-sulfonic acid) 375 nm 480 nm
1-Anilinonaphthalene-8-sulfonic acid (1,8-ANS) 375 nm 479 nm
5-(and-6)-Carboxy-2', 7'-dichlorofluorescein pH 9.0 504 nm 525 nm
5-FAM pH 9.0 492 nm 518 nm
5-ROX (5-Carboxy-X-rhodamine, triethylammonium salt) 578 nm 604 nm
5-ROX pH 7.0 578 nm 604 nm
5-TAMRA 549 nm 577 nm
5-TAMRA pH 7.0 553 nm 576 nm
5-TAMRA-MeOH 543 nm 567 nm
6 JOE 520 nm 548 nm
6,8-Difluoro-7-hydroxy-4-methylcoumarin pH 9.0 358 nm 450 nm
6-Carboxyrhodamine 6G pH 7.0 526 nm 547 nm
6-Carboxyrhodamine 6G, hydrochloride 525 nm 547 nm
6-HEX, SE pH 9.0 534 nm 559 nm
6-TET, SE pH 9.0 521 nm 542 nm
7-Amino-4-methylcoumarin pH 7.0 346 nm 442 nm
7-Hydroxy-4-methylcoumarin 360 nm 447 nm
7-Hydroxy-4-methylcoumarin pH 9.0 361 nm 448 nm
Acridine Orange 431 nm 520 nm
Alexa 350 343 nm 441 nm
Alexa 405 401 nm 421 nm
Alexa 430 431 nm 540 nm
Alexa 488 493 nm 520 nm
Alexa 532 528 nm 553 nm
Alexa 546 562 nm 573 nm
Alexa 555 553 nm 568 nm
Alexa 568 576 nm 603 nm
Alexa 594 590 nm 619 nm
Alexa 633 632 nm 648 nm
Alexa 647 653 nm 669 nm
Alexa 660 664 nm 691 nm
Alexa 680 679 nm 703 nm
Alexa 700 696 nm 720 nm
Alexa Fluor 430 antibody conjugate pH 7.2 431 nm 540 nm
Alexa Fluor 488 antibody conjugate pH 8.0 499 nm 520 nm
Alexa Fluor 488 hydrazide-water 493 nm 518 nm
Alexa Fluor 532 antibody conjugate pH 7.2 528 nm 553 nm
Alexa Fluor 555 antibody conjugate pH 7.2 553 nm 568 nm
Alexa Fluor 568 antibody conjugate pH 7.2 579 nm 603 nm
Alexa Fluor 610 R-phycoerythrin streptavidin pH 7.2 567 nm 627 nm
Alexa Fluor 633 antibody conjugate pH 7.2 631 nm 648 nm
Alexa Fluor 647 antibody conjugate pH 7.2 653 nm 668 nm
Alexa Fluor 647 R-phycoerythrin streptavidin pH 7.2 569 nm 666 nm
Alexa Fluor 660 antibody conjugate pH 7.2 663 nm 691 nm
Alexa Fluor 680 antibody conjugate pH 7.2 679 nm 702 nm
Alexa Fluor 700 antibody conjugate pH 7.2 696 nm 719 nm
Allophycocyanin pH 7.5 651 nm 660 nm
AMCA conjugate 347 nm 444 nm
Amino Coumarin 345 nm 442 nm
APC (allophycocyanin) 650 nm 660 nm
Atto 647 644 nm 670 nm
Auramine O 431 nm 501 nm
BCECF pH 5.5 485 nm 521 nm
BCECF pH 9.0 501 nm 527 nm
BFP (Blue Fluorescent Protein) 380 nm 439 nm
BO-PRO-1-DNA 462 nm 482 nm
BO-PRO-3-DNA 574 nm 604 nm
BOBO-1-DNA 461 nm 484 nm
BOBO-3-DNA 570 nm 605 nm
BODIPY 650/665-X, MeOH 646 nm 664 nm
BODIPY FL conjugate 503 nm 512 nm
BODIPY FL, MeOH 502 nm 511 nm
Bodipy R6G SE 528 nm 547 nm
BODIPY R6G, MeOH 528 nm 547 nm
BODIPY TMR-X antibody conjugate pH 7.2 544 nm 573 nm
Bodipy TMR-X conjugate 544 nm 573 nm
BODIPY TMR-X, MeOH 544 nm 570 nm
BODIPY TMR-X, SE 544 nm 570 nm
BODIPY TR-X phallacidin pH 7.0 590 nm 621 nm
BODIPY TR-X, MeOH 588 nm 621 nm
BODIPY TR-X, SE 588 nm 621 nm
BOPRO-1 462 nm 482 nm
BOPRO-3 574 nm 604 nm
Calcein 493 nm 514 nm
Calcein pH 9.0 494 nm 514 nm
Calcium Crimson 589 nm 608 nm
Calcium Crimson Ca2+ 590 nm 608 nm
Calcium Green 506 nm 530 nm
Calcium Green-1 Ca2+ 506 nm 529 nm
Calcium Orange 549 nm 574 nm
Calcium Orange Ca2+ 549 nm 573 nm
Carboxynaphthofluorescein pH 10.0 600 nm 674 nm
Cascade Blue 398 nm 420 nm
Cascade Blue BSA pH 7.0 401 nm 419 nm
Cascade Yellow 399 nm 549 nm
Cascade Yellow antibody conjugate pH 8.0 399 nm 549 nm
CFDA 495 nm 517 nm
CFP (Cyan Fluorescent Protein) 434 nm 474 nm
CI-NERF pH 2.5 504 nm 541 nm
CI-NERF pH 6.0 513 nm 538 nm
Citrine 515 nm 530 nm
Coumarin 360 nm 447 nm
Cy 2 489 nm 503 nm
Cy 3 549 nm 562 nm
Cy 3.5 578 nm 591 nm
Cy 5 646 nm 664 nm
Cy 5.5 673 nm 692 nm
CyQUANT GR-DNA 502 nm 523 nm
Dansyl Cadaverine 335 nm 524 nm
Dansyl Cadaverine, MeOH 335 nm 526 nm
DAPI 358 nm 463 nm
DAPI-DNA 359 nm 461 nm
Dapoxyl (2-aminoethyl) sulfonamide 372 nm 582 nm
DDAO pH 9.0 648 nm 657 nm
Di-8 ANEPPS 469 nm 630 nm
Di-8-ANEPPS-lipid 469 nm 631 nm
DiI 551 nm 565 nm
DiO 487 nm 501 nm
DM-NERF pH 4.0 493 nm 530 nm
DM-NERF pH 7.0 509 nm 537 nm
DsRed 563 nm 581 nm
DTAF 495 nm 517 nm
dTomato 554 nm 581 nm
DyLight 350 332 nm 435 nm
DyLight 405 399 nm 434 nm
DyLight 488 493 nm 518 nm
DyLight 549 555 nm 569 nm
DyLight 594 592 nm 616 nm
DyLight 633 624 nm 646 nm
DyLight 649 652 nm 668 nm
DyLight 680 678 nm 706 nm
eCFP (Enhanced Cyan Fluorescent Protein) 437 nm 476 nm
eGFP (Enhanced Green Fluorescent Protein) 488 nm 509 nm
Eosin 524 nm 546 nm
Eosin antibody conjugate pH 8.0 525 nm 546 nm
Erythrosin-5-isothiocyanate pH 9.0 533 nm 554 nm
Ethidium Bromide 524 nm 605 nm
Ethidium homodimer 528 nm 617 nm
Ethidium homodimer-1-DNA 528 nm 617 nm
evoglow-Bs1 448 nm 496 nm
evoglow-Bs2 448 nm 496 nm
evoglow-Pp1 448 nm 495 nm
eYFP (Enhanced Yellow Fluorescent Protein) 514 nm 526 nm
FDA 495 nm 517 nm
FITC 495 nm 517 nm
FITC antibody conjugate pH 8.0 495 nm 519 nm
FlAsH 509 nm 529 nm
Fluo-3 506 nm 527 nm
Fluo-3 Ca2+ 506 nm 527 nm
Fluo-4 494 nm 516 nm
Fluor-Ruby 554 nm 582 nm
Fluorescein 495 nm 517 nm
Fluorescein 0.1 M NaOH 493 nm 513 nm
Fluorescein antibody conjugate pH 8.0 493 nm 517 nm
Fluorescein dextran pH 8.0 501 nm 524 nm
Fluorescein pH 9.0 490 nm 514 nm
Fluoro-Emerald 495 nm 524 nm
FM 1-43 472 nm 578 nm
FM 1-43 lipid 473 nm 579 nm
FM 4-64 508 nm 751 nm
FM 4-64, 2% CHAPS 506 nm 751 nm
Fura Red Ca2+ 435 nm 670 nm
Fura Red, high Ca 434 nm 659 nm
Fura Red, low Ca 472 nm 673 nm
Fura-2 Ca2+ 336 nm 505 nm
Fura-2, high Ca 336 nm 504 nm
Fura-2, no Ca 367 nm 515 nm
GFP (S65T) 489 nm 509 nm
HcRed 590 nm 614 nm
Hoechst 33258 352 nm 455 nm
Hoechst 33258-DNA 352 nm 455 nm
Hoechst 33342 352 nm 455 nm
Indo-1 Ca2+ 346 nm 404 nm
Indo-1, Ca free 346 nm 479 nm
Indo-1, Ca saturated 331 nm 404 nm
JC-1 592 nm 595 nm
JC-1 pH 8.2 593 nm 595 nm
Lissamine rhodamine 572 nm 590 nm
LOLO-1-DNA 568 nm 580 nm
Lucifer Yellow, CH 428 nm 542 nm
LysoSensor Blue 374 nm 424 nm
LysoSensor Blue pH 5.0 374 nm 424 nm
LysoSensor Green 447 nm 504 nm
LysoSensor Green pH 5.0 447 nm 502 nm
LysoSensor Yellow pH 3.0 389 nm 542 nm
LysoSensor Yellow pH 9.0 335 nm 530 nm
LysoTracker Blue 373 nm 421 nm
LysoTracker Green 503 nm 509 nm
LysoTracker Red 578 nm 589 nm
Magnesium Green 507 nm 530 nm
Magnesium Green Mg2+ 507 nm 531 nm
Magnesium Orange 550 nm 575 nm
Marina Blue 362 nm 464 nm
mBanana 540 nm 553 nm
mCherry 587 nm 610 nm
mHoneydew 478 nm 562 nm
MitoTracker Green 490 nm 512 nm
MitoTracker Green FM, MeOH 490 nm 512 nm
MitoTracker Orange 551 nm 575 nm
MitoTracker Orange, MeOH 551 nm 575 nm
MitoTracker Red 578 nm 599 nm
MitoTracker Red, MeOH 578 nm 599 nm
mNeonGreen 506 nm 517 nm
mOrange 548 nm 562 nm
mPlum 587 nm 649 nm
mRFP 585 nm 608 nm
mStrawberry 575 nm 596 nm
mTangerine 568 nm 585 nm
NBD-X 466 nm 534 nm
NBD-X, MeOH 467 nm 538 nm
NeuroTrace 500/525, green fluorescent Nissl stain-RNA 497 nm 524 nm
Nile Blue, EtOH 631 nm 660 nm
Nile Red 559 nm 637 nm
Nile Red-lipid 553 nm 636 nm
Nissl 497 nm 524 nm
Oregon Green 488 498 nm 526 nm
Oregon Green 488 antibody conjugate pH 8.0 498 nm 526 nm
Oregon Green 514 512 nm 532 nm
Oregon Green 514 antibody conjugate pH 8.0 513 nm 533 nm
Pacific Blue 404 nm 455 nm
Pacific Blue antibody conjugate pH 8.0 404 nm 455 nm
Phycoerythrin 565 nm 575 nm
PicoGreen dsDNA quantitation reagent 502 nm 522 nm
PO-PRO-1 434 nm 457 nm
PO-PRO-1-DNA 435 nm 457 nm
PO-PRO-3 539 nm 571 nm
PO-PRO-3-DNA 539 nm 571 nm
POPO-1 433 nm 457 nm
POPO-1-DNA 433 nm 458 nm
POPO-3 533 nm 573 nm
Propidium Iodide 538 nm 617 nm
Propidium Iodide-DNA 538 nm 619 nm
Quasar 570 549 nm 562 nm
R-Phycoerythrin pH 7.5 565 nm 576 nm
ReAsH 597 nm 608 nm
Resorufin 571 nm 584 nm
Resorufin pH 9.0 571 nm 584 nm
Rhod-2 552 nm 577 nm
Rhod-2 Ca2+ 553 nm 578 nm
Rhodamine 551 nm 573 nm
Rhodamine 110 497 nm 520 nm
Rhodamine 110 pH 7.0 497 nm 520 nm
Rhodamine 123, MeOH 507 nm 529 nm
Rhodamine B 543 nm 565 nm
Rhodamine Green 497 nm 524 nm
Rhodamine phalloidin pH 7.0 558 nm 575 nm
Rhodamine Red-X antibody conjugate pH 8.0 573 nm 591 nm
Rhodaminen Green pH 7.0 497 nm 523 nm
Rhodol Green antibody conjugate pH 8.0 499 nm 524 nm
Sapphire 396 nm 511 nm
SBFI-Na+ 336 nm 527 nm
Sodium Green Na+ 507 nm 531 nm
Sulforhodamine 101, EtOH 578 nm 593 nm
SYBR Green I 498 nm 522 nm
SYPRO Ruby 467 nm 618 nm
SYTO 13-DNA 488 nm 506 nm
SYTO 17-DNA 619 nm 638 nm
SYTO 45-DNA 451 nm 486 nm
SYTOX Blue-DNA 445 nm 470 nm
Tetramethylrhodamine antibody conjugate pH 8.0 552 nm 578 nm
Tetramethylrhodamine dextran pH 7.0 555 nm 582 nm
Texas Red-X antibody conjugate pH 7.2 596 nm 613 nm
TO-PRO-1-DNA 515 nm 531 nm
TO-PRO-3-DNA 642 nm 657 nm
TOTO-1-DNA 514 nm 531 nm
TOTO-3-DNA 642 nm 661 nm
TRITC 550 nm 573 nm
X-Rhod-1 Ca2+ 580 nm 602 nm
YO-PRO-1-DNA 491 nm 507 nm
YO-PRO-3-DNA 613 nm 629 nm
YOYO-1-DNA 491 nm 509 nm
YOYO-3-DNA 612 nm 631 nm